Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   JG532_RS02475 Genome accession   NZ_CP066748
Coordinates   507017..508141 (+) Length   374 a.a.
NCBI ID   WP_000228544.1    Uniprot ID   Q8X8F4
Organism   Escherichia coli O157:H7 strain ECP19-2498     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 502017..513141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JG532_RS02450 (JG532_02450) mscL 502143..502553 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  JG532_RS02455 (JG532_02455) trkA 502683..504059 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  JG532_RS02460 (JG532_02460) rsmB 504081..505370 (-) 1290 WP_001301795.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  JG532_RS02465 (JG532_02465) fmt 505416..506363 (-) 948 WP_000004459.1 methionyl-tRNA formyltransferase -
  JG532_RS02470 (JG532_02470) def 506378..506887 (-) 510 WP_000114984.1 peptide deformylase -
  JG532_RS02475 (JG532_02475) dprA 507017..508141 (+) 1125 WP_000228544.1 DNA-protecting protein DprA Machinery gene
  JG532_RS02480 (JG532_02480) smg 508113..508586 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  JG532_RS02485 (JG532_02485) yrdD 508615..509157 (+) 543 WP_001129722.1 type I DNA topoisomerase -
  JG532_RS02490 (JG532_02490) tsaC 509162..509734 (+) 573 WP_001301437.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  JG532_RS02495 (JG532_02495) aroE 509739..510557 (+) 819 WP_000451254.1 shikimate dehydrogenase -
  JG532_RS02500 (JG532_02500) yrdB 510554..510811 (+) 258 WP_001070590.1 DUF1488 domain-containing protein -
  JG532_RS02505 (JG532_02505) yrdA 510787..511341 (-) 555 WP_001286216.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 41005.96 Da        Isoelectric Point: 6.2655

>NTDB_id=456047 JG532_RS02475 WP_000228544.1 507017..508141(+) (dprA) [Escherichia coli O157:H7 strain ECP19-2498]
MVDTEIWLRLMSISSLYGDDMVRIAHWLARQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLAKHGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLAASLLEQGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=456047 JG532_RS02475 WP_000228544.1 507017..508141(+) (dprA) [Escherichia coli O157:H7 strain ECP19-2498]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGTATCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAGACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGGAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTGGCGACGACAGATTACCCCGGTGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGAAGCATGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTAGCGCATAAAGCAGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTGGGGAATGGACTTAATACCATTCATCCCCGCCGTCATGCCCGACTGGCTGCCAGTCTGCT
TGAACAGGGGGGCGCTCTCGTCTCGGAATTTCCCCTCGATGTTCCACCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGTAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGAGAAGTTTTTGCCTTGCCAGGTCCAATAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTTACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X8F4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.78

98.663

0.481
  dprA Glaesserella parasuis strain SC1401

45.401

90.107

0.409
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

49.355

82.888

0.409
  dprA Vibrio cholerae strain A1552

49.355

82.888

0.409
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

44.545

88.235

0.393