Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   HUZ72_RS22575 Genome accession   NZ_CP055037
Coordinates   4662404..4663924 (+) Length   506 a.a.
NCBI ID   WP_000058966.1    Uniprot ID   A0A061KPU8
Organism   Shigella flexneri strain SWHEFF_60     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4657404..4668924
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUZ72_RS22550 (HUZ72_22360) ilvE 4658958..4659887 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  HUZ72_RS22555 (HUZ72_22365) ilvM 4659907..4660170 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  HUZ72_RS22560 (HUZ72_22370) ilvG 4660167..4661813 (-) 1647 WP_001012620.1 acetolactate synthase 2 catalytic subunit -
  HUZ72_RS22565 (HUZ72_22375) ilvX 4661816..4661866 (-) 51 WP_001387183.1 peptide IlvX -
  HUZ72_RS22570 (HUZ72_22380) ilvL 4661953..4662051 (-) 99 WP_001311244.1 ilv operon leader peptide -
  HUZ72_RS22575 (HUZ72_22385) comM 4662404..4663924 (+) 1521 WP_000058966.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  HUZ72_RS22580 (HUZ72_22390) maoP 4663949..4664287 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  HUZ72_RS22585 (HUZ72_22395) hdfR 4664406..4665245 (+) 840 WP_000379245.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55213.40 Da        Isoelectric Point: 7.4105

>NTDB_id=455906 HUZ72_RS22575 WP_000058966.1 4662404..4663924(+) (comM) [Shigella flexneri strain SWHEFF_60]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEHGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=455906 HUZ72_RS22575 WP_000058966.1 4662404..4663924(+) (comM) [Shigella flexneri strain SWHEFF_60]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTGAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATCAACCTTGCTCCAGCCGATCTACCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGCGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAACATGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGGCCTCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGTGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCATCGTTAACTGCGATGGTAGGCGGTGGCGCAATTCCAGGGCCCGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGTTACCTGAATTTGAAAGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACGCGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACACCAGAGCAAACGCTGCGTTATCTCAACCGACTCTCTGGTC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCGCCTCCCGGTATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCGCCACCGTTAAACAACGCGTAATGGCCGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAACTGAACGCCTG
GCTGGATAGTCCGGAAATACGCCAATTCTGCAAACTTGAGAGCGAAGATGCGATGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAACATTTGCAGGAGGCAGTTAGCTATCGAGCGATTGACCGTTTGCTCATCCATCTGCAGAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A061KPU8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.904

100

0.595
  comM Glaesserella parasuis strain SC1401

58.777

100

0.589
  comM Vibrio cholerae strain A1552

59.163

99.209

0.587
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Legionella pneumophila str. Paris

48.394

98.419

0.476
  comM Legionella pneumophila strain ERS1305867

48.394

98.419

0.476
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443