Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   I5P57_RS03805 Genome accession   NZ_CP066740
Coordinates   788451..789944 (+) Length   497 a.a.
NCBI ID   WP_000249335.1    Uniprot ID   -
Organism   Escherichia coli strain R12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 783451..794944
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5P57_RS03785 (I5P57_00748) pilD 784099..784908 (+) 810 WP_000895875.1 prepilin peptidase PppA Machinery gene
  I5P57_RS03790 (I5P57_00749) gspS2 784974..785384 (+) 411 WP_001298257.1 type II secretion system pilot lipoprotein GspS-beta -
  I5P57_RS03795 (I5P57_00750) gspC 785402..786361 (+) 960 WP_001764943.1 type II secretion system protein GspC -
  I5P57_RS03800 (I5P57_00751) gspD 786391..788451 (+) 2061 WP_000498844.1 type II secretion system secretin GspD -
  I5P57_RS03805 (I5P57_00752) pilB/pilB1 788451..789944 (+) 1494 WP_000249335.1 type II secretion system ATPase GspE Machinery gene
  I5P57_RS03810 (I5P57_00753) gspF 789944..791167 (+) 1224 WP_001173412.1 type II secretion system inner membrane protein GspF -
  I5P57_RS03815 (I5P57_00754) gspG 791184..791639 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  I5P57_RS03820 (I5P57_00755) gspH 791643..792206 (+) 564 WP_001764944.1 type II secretion system minor pseudopilin GspH -
  I5P57_RS03825 (I5P57_00756) gspI 792203..792574 (+) 372 WP_000820086.1 type II secretion system minor pseudopilin GspI -
  I5P57_RS03830 (I5P57_00757) gspJ 792571..793176 (+) 606 WP_001250460.1 type II secretion system minor pseudopilin GspJ -
  I5P57_RS03835 (I5P57_00758) gspK 793173..794150 (+) 978 WP_000633220.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55129.20 Da        Isoelectric Point: 5.9800

>NTDB_id=455790 I5P57_RS03805 WP_000249335.1 788451..789944(+) (pilB/pilB1) [Escherichia coli strain R12]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSHELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCSDCRQAAPA
TDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=455790 I5P57_RS03805 WP_000249335.1 788451..789944(+) (pilB/pilB1) [Escherichia coli strain R12]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTACAACGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAGGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAAGCTCGCCAGCTGATGGAAGATCTCGGTTCCGCCGAGGACTTTTTTACCCTCGCTGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCAATCAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCAACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTGAGTCACGAACTGACC
GCGCAGTTGCGTCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACCCTGCATACCAACACAGCGGTGGGGGCGATCACGCGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTTTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGCTCCGACTGCCGTCAGGCCGCGCCTGCC
ACTGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATCGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.741

100

0.433
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

47.859

79.879

0.382
  pilB Acinetobacter baumannii D1279779

47.118

80.282

0.378
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

46.97

79.678

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilB Acinetobacter baylyi ADP1

46.667

78.471

0.366
  pilF Thermus thermophilus HB27

45

80.483

0.362