Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   HUB32_RS14940 Genome accession   NZ_CP054468
Coordinates   3323378..3324883 (-) Length   501 a.a.
NCBI ID   WP_046577596.1    Uniprot ID   -
Organism   Burkholderia gladioli strain CGB10     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3318378..3329883
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HUB32_RS14915 - 3319370..3320005 (-) 636 WP_013699412.1 LON peptidase substrate-binding domain-containing protein -
  HUB32_RS14920 rapZ 3320038..3320931 (-) 894 WP_013699411.1 RNase adapter RapZ -
  HUB32_RS14925 hprK 3321042..3322010 (-) 969 WP_013699410.1 HPr(Ser) kinase/phosphatase -
  HUB32_RS14930 - 3322136..3322648 (-) 513 WP_025097001.1 PTS sugar transporter subunit IIA -
  HUB32_RS14935 hpf 3322862..3323227 (-) 366 WP_013699408.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  HUB32_RS14940 rpoN/rpoN1 3323378..3324883 (-) 1506 WP_046577596.1 RNA polymerase factor sigma-54 Machinery gene
  HUB32_RS14945 lptB 3325073..3325849 (-) 777 WP_013699406.1 LPS export ABC transporter ATP-binding protein -
  HUB32_RS14950 lptA 3325846..3326514 (-) 669 WP_036049142.1 lipopolysaccharide transport periplasmic protein LptA -
  HUB32_RS14955 lptC 3326533..3327147 (-) 615 WP_017918294.1 LPS export ABC transporter periplasmic protein LptC -
  HUB32_RS14960 - 3327151..3327690 (-) 540 WP_013699403.1 HAD family hydrolase -
  HUB32_RS14965 kdsD 3327690..3328673 (-) 984 WP_025096995.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 55103.08 Da        Isoelectric Point: 4.9483

>NTDB_id=452666 HUB32_RS14940 WP_046577596.1 3323378..3324883(-) (rpoN/rpoN1) [Burkholderia gladioli strain CGB10]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDEWIASPLRVAADGSLIAQSPPSSTPEPMHG
GGSNGSEPSGDRAERDDSRNGDDYDYNADSGDSGQWNLDDYGRSSGASDDDDLPPLQVHEASTSLREHLSAQLRVTQAGP
RDRALVMFLIESLDDDGYLGAGLDEVLTDLPEELEVDLDELGAALALLHSFDPAGVGARSASECLRLQLLRLDPSPTRTL
SLDIVSQHLELLAARDFTRLRKQLKANDDELREAHALIRSLEPFPGAAYGKTEADYVVPDIMVRKSGQNWLAELNPEVVP
KLRINHLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDQKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=452666 HUB32_RS14940 WP_046577596.1 3323378..3324883(-) (rpoN/rpoN1) [Burkholderia gladioli strain CGB10]
ATGAAAGCCAGCCTCCAACTTCGCCTGTCGCAGCACCTCGCGCTCACGCCCCAGCTCCAGCAGTCGATCCGGCTGCTGCA
GCTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCCCAGAATCCGCTGCTCGAGAACGACGAATGGATCG
CTAGCCCGCTGCGGGTGGCCGCCGACGGCTCGCTGATCGCGCAGAGCCCGCCCTCCTCGACGCCCGAGCCGATGCACGGT
GGCGGCTCGAACGGCAGCGAACCCTCGGGCGATCGCGCCGAACGCGACGACTCGCGCAATGGCGACGACTACGACTACAA
CGCCGACAGCGGCGATTCGGGTCAGTGGAATCTCGACGACTACGGTCGCTCCTCGGGCGCCTCCGACGACGACGACCTCC
CGCCGCTGCAGGTGCACGAGGCCTCGACCTCGCTGCGCGAGCATCTGTCCGCGCAATTGCGGGTGACCCAGGCGGGTCCG
CGCGACCGCGCGCTGGTGATGTTCCTGATCGAATCGCTCGACGACGACGGTTATCTCGGCGCGGGGCTCGACGAGGTGTT
GACCGACCTGCCCGAGGAACTGGAAGTCGATCTCGACGAACTGGGCGCCGCGCTGGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTGGGGGCGCGCTCCGCATCGGAATGCTTGCGCCTGCAATTGTTGCGACTCGATCCGTCCCCCACGCGCACGCTG
TCGCTCGACATCGTCTCGCAGCATCTCGAATTGCTGGCCGCGCGCGACTTCACGCGGCTGCGCAAGCAGTTGAAGGCCAA
CGACGACGAGCTGCGCGAGGCCCACGCACTGATCCGCTCGCTCGAGCCCTTCCCGGGCGCCGCCTACGGCAAGACCGAGG
CGGACTACGTGGTGCCCGACATCATGGTCAGGAAGAGCGGCCAGAACTGGCTCGCGGAGCTCAATCCCGAGGTGGTGCCG
AAGCTGCGCATCAACCACCTGTACGCCAATATCCTGCGCAACAGCCGGGGCGATCCGAGCGCCGGTTCGCTCAAGCAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGAGACCATCCTGCGTGTCGCGCAGGCCATCGTGG
AGCGTCAGAAGAACTTTTTTGCGCATGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCTCCCGTGTGACGACCGGCAAGTACATGCTGACCCCGTTCGGCACTCTTGAATTTAAGTACTT
CTTCGGATCGCACGTGTCGACGGATACCGGGGGCGCTGCCTCTTCCACGGCAATCCGCGCCCTCATCAAGCAACTGATAG
GAGCCGAAGACCAGAAATCTCCTCTTTCGGACAGCCGAATAGCCGAGCTGCTGGCGGAACAAGGCTTCGTGGTCGCGCGC
CGCACCGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCCGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689