Detailed information NTG0452483

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: I6H98_RS18830
• Genome accession: NZ_CP065966
• Coordinates: 4035974..4037122 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain FDAARGOS_1041
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4030974..4042122

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I6H98_RS18805 (I6H98_18805)	-	4032125..4032421 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
I6H98_RS18810 (I6H98_18810)	-	4032418..4033011 (-)	594	WP_003084544.1	YggT family protein	-
I6H98_RS18815 (I6H98_18815)	proC	4033022..4033843 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
I6H98_RS18820 (I6H98_18820)	-	4033855..4034547 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
I6H98_RS18825 (I6H98_18825)	pilT	4034762..4035796 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
I6H98_RS18830 (I6H98_18830)	pilU	4035974..4037122 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
I6H98_RS18835 (I6H98_18835)	czcD	4037130..4038029 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
I6H98_RS18840 (I6H98_18840)	-	4038184..4038588 (+)	405	WP_003084560.1	NINE protein	-
I6H98_RS18845 (I6H98_18845)	-	4038831..4040204 (+)	1374	WP_003459266.1	pyridoxal-phosphate dependent enzyme	-
I6H98_RS18850 (I6H98_18850)	-	4040201..4041385 (+)	1185	WP_016852373.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=452483 I6H98_RS18830 WP_003100959.1 4035974..4037122(+) (pilU) [Pseudomonas aeruginosa strain FDAARGOS_1041]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=452483 I6H98_RS18830 WP_003100959.1 4035974..4037122(+) (pilU) [Pseudomonas aeruginosa strain FDAARGOS_1041]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGCCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382