Detailed information NTG0451107

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: HQN60_RS05095
• Genome accession: NZ_CP054143
• Coordinates: 1057480..1058664 (+)
• Length: 394 a.a.
• NCBI ID: WP_173532644.1
• Uniprot ID: A0A6M8SRM8
• Organism: Deefgea piscis strain D17
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1052480..1063664

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HQN60_RS05080 (HQN60_05080)	-	1052942..1053631 (-)	690	WP_173532641.1	metallophosphoesterase	-
HQN60_RS05085 (HQN60_05085)	-	1053864..1055117 (+)	1254	WP_173532642.1	Hsp70 family protein	-
HQN60_RS05090 (HQN60_05090)	-	1055373..1057241 (+)	1869	WP_173532643.1	DEAD/DEAH box helicase	-
HQN60_RS05095 (HQN60_05095)	pilU	1057480..1058664 (+)	1185	WP_173532644.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
HQN60_RS05100 (HQN60_05100)	-	1058661..1059158 (-)	498	WP_173532645.1	GNAT family N-acetyltransferase	-
HQN60_RS05105 (HQN60_05105)	-	1059329..1059868 (-)	540	WP_173532646.1	DUF1523 family protein	-
HQN60_RS05110 (HQN60_05110)	-	1060202..1060531 (+)	330	WP_173532647.1	zinc ribbon domain-containing protein YjdM	-
HQN60_RS05115 (HQN60_05115)	gnd	1060654..1061565 (-)	912	WP_173532648.1	phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating)	-
HQN60_RS05120 (HQN60_05120)	-	1061748..1062761 (+)	1014	WP_173532649.1	peptidase U32 family protein	-

Sequence

Protein

• Length: 394 a.a.
• Molecular weight: 44009.66 Da
• Isoelectric Point: 6.5063

>NTDB_id=451107 HQN60_RS05095 WP_173532644.1 1057480..1058664(+) (pilU) [Deefgea piscis strain D17]
MSPEQAKKTMHDLLRMMIQHKASDLFIADDFPPAMKINGRMTPIGAQKLNAAATRALALAIMRPDQQTEFAQELECNFAI
NPEGIGRFRVNVFMQQEKVGMVLRTITSKIPNFDELGLPKVLKEVMMSQRGLVLLVGGTGSGKSTTMAAMIDYRNENSHG
HIITIEDPIEYVHKNKNCLVTHREVGRDTKNWFAALKNTLRQAPDVILIGEIRDRETMEYAMAFAETGHLCMGTLHANSS
NQALERIVNFFPEERHPQLYMDLSLNMRGIISQRLVATPDGKGRSAAIEILLNTPLVADMIFKGDVGGVKTAMAKSKELG
MQTFDMALFALYEAGKISMEEALKNADSLNELRLNIKLNSLHHRKEEHHSGLEHLTLEAQQVPEEEAETSESSN

Nucleotide

• Length: 1185 bp

>NTDB_id=451107 HQN60_RS05095 WP_173532644.1 1057480..1058664(+) (pilU) [Deefgea piscis strain D17]
ATGAGCCCCGAACAAGCTAAAAAAACCATGCATGACTTACTGCGGATGATGATTCAACACAAAGCATCCGATTTGTTTAT
TGCCGATGATTTTCCGCCAGCCATGAAGATTAACGGCAGAATGACGCCGATTGGCGCGCAAAAGCTCAATGCGGCGGCCA
CGCGAGCGCTGGCTTTGGCCATTATGCGTCCTGATCAACAAACCGAATTTGCACAAGAATTAGAATGCAATTTTGCGATT
AACCCCGAAGGCATCGGTCGCTTTCGGGTGAATGTCTTTATGCAGCAAGAAAAGGTCGGCATGGTGCTGCGAACCATCAC
CAGCAAGATTCCTAATTTTGATGAATTAGGGCTGCCCAAAGTATTAAAAGAAGTGATGATGAGTCAGCGTGGTTTGGTGC
TATTGGTTGGCGGTACCGGCTCGGGTAAGTCGACGACGATGGCGGCGATGATTGATTACCGCAATGAAAATAGCCACGGC
CACATCATTACCATCGAAGACCCGATTGAGTATGTGCATAAAAATAAAAATTGCTTGGTCACGCACCGCGAAGTGGGCCG
TGATACCAAAAATTGGTTTGCTGCGCTCAAAAATACGCTGCGCCAAGCGCCTGACGTGATTTTAATTGGCGAAATTCGCG
ACCGAGAAACCATGGAATACGCCATGGCGTTCGCCGAAACCGGCCATCTTTGTATGGGCACTTTGCACGCCAATAGCTCT
AATCAAGCGTTGGAGCGCATCGTGAATTTCTTCCCCGAAGAGCGCCACCCGCAGCTGTATATGGATTTATCGCTCAATAT
GCGCGGGATTATTTCGCAGCGTTTGGTGGCAACACCCGATGGCAAAGGACGTAGCGCCGCAATTGAAATCTTGCTCAATA
CGCCGCTGGTTGCCGATATGATTTTTAAAGGCGATGTGGGCGGCGTTAAAACCGCGATGGCCAAGTCTAAAGAATTGGGC
ATGCAAACCTTTGATATGGCGCTGTTTGCGCTGTATGAAGCCGGCAAAATCAGTATGGAAGAGGCATTAAAGAATGCCGA
TTCACTCAATGAGCTGCGCTTAAATATTAAACTCAACAGCTTGCATCATCGTAAAGAAGAGCATCATAGCGGACTTGAGC
ATCTCACCCTTGAAGCGCAGCAAGTGCCCGAAGAAGAGGCCGAGACCAGCGAAAGCAGCAACTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (87-276)

3D structure

• AlphaFold DB: A0A6M8SRM8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.621	88.325	0.518
pilU	Acinetobacter baylyi ADP1	56.125	89.086	0.5
pilU	Vibrio cholerae strain A1552	53.521	90.102	0.482
pilT	Pseudomonas aeruginosa PAK	43.582	85.025	0.371
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.433	85.533	0.363