Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HRJ47_RS01535 Genome accession   NZ_CP054137
Coordinates   323410..324162 (+) Length   250 a.a.
NCBI ID   WP_060464993.1    Uniprot ID   A0A1E2YH36
Organism   Acinetobacter pittii strain JXA13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 318410..329162
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HRJ47_RS01520 (HRJ47_01520) ponA 318991..321552 (-) 2562 WP_032052651.1 penicillin-binding protein PBP1a -
  HRJ47_RS01525 (HRJ47_01525) pilM 321714..322772 (+) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  HRJ47_RS01530 (HRJ47_01530) pilN 322772..323413 (+) 642 WP_086399992.1 PilN domain-containing protein Machinery gene
  HRJ47_RS01535 (HRJ47_01535) pilO 323410..324162 (+) 753 WP_060464993.1 type 4a pilus biogenesis protein PilO Machinery gene
  HRJ47_RS01540 (HRJ47_01540) pilP 324173..324700 (+) 528 WP_086399364.1 pilus assembly protein PilP Machinery gene
  HRJ47_RS01545 (HRJ47_01545) pilQ 324763..326928 (+) 2166 WP_203080013.1 type IV pilus secretin PilQ Machinery gene
  HRJ47_RS01550 (HRJ47_01550) aroK 326940..327509 (+) 570 WP_002116987.1 shikimate kinase AroK -
  HRJ47_RS01555 (HRJ47_01555) aroB 327538..328620 (+) 1083 WP_005067096.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28241.10 Da        Isoelectric Point: 4.8782

>NTDB_id=451025 HRJ47_RS01535 WP_060464993.1 323410..324162(+) (pilO) [Acinetobacter pittii strain JXA13]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAVENPEHQEKNASASSVAP
DSSTANTQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=451025 HRJ47_RS01535 WP_060464993.1 323410..324162(+) (pilO) [Acinetobacter pittii strain JXA13]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGATATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTATTGGCTTTGGGGTATTTTGTTGCTATTCAGCCAAAGCTACAAGCAATAGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAGGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGTGCTGTAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACACACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E2YH36

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

69.583

96

0.668