Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   I6G22_RS06735 Genome accession   NZ_CP065737
Coordinates   1258371..1259372 (+) Length   333 a.a.
NCBI ID   WP_003131994.1    Uniprot ID   Q9CDL3
Organism   Lactococcus lactis strain FDAARGOS_865     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1253371..1264372
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I6G22_RS06720 (I6G22_06725) - 1254744..1255439 (+) 696 WP_025017068.1 hypothetical protein -
  I6G22_RS06725 (I6G22_06730) hexB 1255568..1257538 (+) 1971 WP_044009590.1 DNA mismatch repair endonuclease MutL Machinery gene
  I6G22_RS06730 (I6G22_06735) ruvA 1257651..1258244 (+) 594 WP_003131995.1 Holliday junction branch migration protein RuvA Machinery gene
  I6G22_RS06735 (I6G22_06740) ruvB 1258371..1259372 (+) 1002 WP_003131994.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  I6G22_RS06740 (I6G22_06745) - 1259509..1260393 (+) 885 WP_023190208.1 XRE/MutR family transcriptional regulator -
  I6G22_RS06745 (I6G22_06750) - 1260613..1260903 (+) 291 WP_023190207.1 hypothetical protein -
  I6G22_RS06750 (I6G22_06755) - 1260970..1262154 (+) 1185 WP_014570851.1 ABC transporter permease -
  I6G22_RS06755 (I6G22_06760) - 1262151..1262696 (+) 546 WP_023190206.1 AAA family ATPase -
  I6G22_RS06760 (I6G22_06765) - 1262873..1263037 (+) 165 WP_003131988.1 hypothetical protein -
  I6G22_RS06765 (I6G22_06770) - 1263181..1263618 (+) 438 WP_010906378.1 low molecular weight protein-tyrosine-phosphatase -
  I6G22_RS06770 (I6G22_06775) - 1263719..1264084 (+) 366 WP_003131985.1 MerR family transcriptional regulator -

Sequence

Protein


Download         Length: 333 a.a.        Molecular weight: 37633.17 Da        Isoelectric Point: 4.5825

>NTDB_id=451023 I6G22_RS06735 WP_003131994.1 1258371..1259372(+) (ruvB) [Lactococcus lactis strain FDAARGOS_865]
MNDILNKEPMEEDYGIEKSLRPQFFNQYIGQDKVKEQLEIFIKAAKMREEVLDHVLLFGPPGLGKTTMAFVIANELGVNI
KQTAGPAIEKPGDLVAILNELEPGDVLFIDEIHRMPMQVEEVLYSAMEDFYIDIMLGSGDGSRSVHLDLPPFTLVGATTR
AGMLSNPLRARFGISSHMEYYQERDLEEIVKRTADIFEVEVIDNAALEIALRSRGTPRIANRLLKRVRDFAQIMGDGRVD
KAITDKALSILDVDAAGLDYIDQKILRTMIEMYHGGPVGIGTLAVNIAEDRETVEDMYEPYLIQKGFLMRTKQGRKVTQR
AYEHLGYVYNEED

Nucleotide


Download         Length: 1002 bp        

>NTDB_id=451023 I6G22_RS06735 WP_003131994.1 1258371..1259372(+) (ruvB) [Lactococcus lactis strain FDAARGOS_865]
ATGAACGATATATTAAACAAAGAGCCAATGGAAGAAGATTACGGTATTGAAAAATCTCTACGTCCGCAATTTTTTAATCA
ATATATTGGTCAAGATAAAGTCAAAGAACAATTAGAAATCTTTATTAAAGCAGCAAAAATGCGTGAAGAAGTTTTGGACC
ACGTGTTACTTTTTGGCCCTCCGGGACTTGGTAAGACAACAATGGCATTTGTCATTGCCAATGAGTTGGGGGTTAATATT
AAACAAACAGCGGGTCCTGCCATTGAAAAGCCAGGAGATTTAGTTGCGATTTTGAATGAATTGGAACCTGGCGATGTGCT
ATTTATTGATGAAATTCATCGGATGCCAATGCAAGTTGAAGAAGTTCTTTATAGCGCTATGGAAGATTTCTACATTGATA
TTATGCTTGGTTCTGGTGATGGTTCACGCTCGGTTCATTTAGATTTGCCGCCATTTACTCTAGTTGGGGCAACAACAAGA
GCTGGAATGCTTTCGAATCCATTGCGTGCCCGTTTTGGAATTTCAAGTCATATGGAGTATTATCAAGAGCGTGATTTGGA
AGAAATTGTTAAAAGAACAGCTGATATTTTTGAAGTCGAAGTTATTGATAATGCCGCTTTAGAGATTGCTTTACGTAGTC
GAGGGACTCCGCGGATTGCCAATCGTTTACTTAAACGAGTGCGTGATTTTGCTCAAATAATGGGGGATGGCCGAGTGGAT
AAAGCAATTACCGATAAGGCCTTGTCTATTCTTGATGTTGATGCCGCTGGTTTGGACTATATTGACCAAAAAATTCTCCG
CACAATGATTGAAATGTACCACGGTGGTCCTGTTGGGATTGGAACTTTGGCGGTAAACATTGCCGAAGACCGTGAAACGG
TCGAGGATATGTATGAACCTTATTTGATTCAAAAAGGATTTTTGATGAGAACTAAACAGGGACGTAAGGTGACCCAAAGA
GCCTATGAACACCTAGGTTATGTTTACAATGAAGAAGATTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(256-325)

RuvB AAA lid domain (AAA_lid_4)

(181-254)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(20-178)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q9CDL3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Streptococcus pneumoniae R6

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae D39

78.614

99.7

0.784
  ruvB Streptococcus pneumoniae TIGR4

78.313

99.7

0.781
  ruvB Bacillus subtilis subsp. subtilis str. 168

61.491

96.697

0.595
  ruvB Helicobacter pylori 26695

51.242

96.697

0.495
  ruvB Synechocystis sp. PCC 6803

51.613

93.093

0.48