Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HPY10_RS01335 Genome accession   NZ_CP053822
Coordinates   282286..283302 (+) Length   338 a.a.
NCBI ID   WP_071171299.1    Uniprot ID   -
Organism   Vibrio cholerae strain E7G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 277286..288302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY10_RS01320 (HPY10_01320) - 277827..278558 (-) 732 WP_000495223.1 glutathione peroxidase -
  HPY10_RS01325 (HPY10_01325) oxyR 278698..279591 (+) 894 WP_046121319.1 DNA-binding transcriptional regulator OxyR -
  HPY10_RS01330 (HPY10_01330) - 279666..282143 (-) 2478 WP_000668739.1 penicillin-binding protein 1A -
  HPY10_RS01335 (HPY10_01335) pilM 282286..283302 (+) 1017 WP_071171299.1 type IV pilus assembly protein PilM Machinery gene
  HPY10_RS01340 (HPY10_01340) pilN 283292..283876 (+) 585 WP_000902746.1 PilN domain-containing protein Machinery gene
  HPY10_RS01345 (HPY10_01345) pilO 283869..284459 (+) 591 WP_000157690.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY10_RS01350 (HPY10_01350) pilP 284449..284967 (+) 519 WP_032472751.1 pilus assembly protein PilP Machinery gene
  HPY10_RS01355 (HPY10_01355) pilQ 284993..286708 (+) 1716 WP_175245885.1 type IV pilus secretin PilQ Machinery gene
  HPY10_RS01360 (HPY10_01360) aroK 286895..287419 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37885.60 Da        Isoelectric Point: 7.3329

>NTDB_id=447873 HPY10_RS01335 WP_071171299.1 282286..283302(+) (pilM) [Vibrio cholerae strain E7G]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVQVTEKSLVRSSTTTFQVYATKKDVVDSRWHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHRMLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDALYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=447873 HPY10_RS01335 WP_071171299.1 282286..283302(+) (pilM) [Vibrio cholerae strain E7G]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCCAAGTGACGGAAAAAAGCCTCGTCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTGGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCGAATGTTACACGATCCCACGCAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGCGCTTTATCAACGCTTGTCATTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)

Type IV pilus assembly protein PilM; (PilM_2)

(240-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

98.521

100

0.985
  pilM Vibrio campbellii strain DS40M4

47.181

99.704

0.47