Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HPY05_RS01300 Genome accession   NZ_CP053810
Coordinates   278115..279131 (+) Length   338 a.a.
NCBI ID   WP_175246731.1    Uniprot ID   -
Organism   Vibrio cholerae strain W6G     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 273115..284131
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HPY05_RS01285 (HPY05_01285) - 273656..274387 (-) 732 WP_000495223.1 glutathione peroxidase -
  HPY05_RS01290 (HPY05_01290) oxyR 274527..275420 (+) 894 WP_001025909.1 DNA-binding transcriptional regulator OxyR -
  HPY05_RS01295 (HPY05_01295) - 275495..277972 (-) 2478 WP_000668735.1 penicillin-binding protein 1A -
  HPY05_RS01300 (HPY05_01300) pilM 278115..279131 (+) 1017 WP_175246731.1 type IV pilus assembly protein PilM Machinery gene
  HPY05_RS01305 (HPY05_01305) pilN 279121..279705 (+) 585 WP_057555158.1 PilN domain-containing protein Machinery gene
  HPY05_RS01310 (HPY05_01310) pilO 279698..280288 (+) 591 WP_000157688.1 type 4a pilus biogenesis protein PilO Machinery gene
  HPY05_RS01315 (HPY05_01315) pilP 280278..280796 (+) 519 WP_000792032.1 pilus assembly protein PilP Machinery gene
  HPY05_RS01320 (HPY05_01320) pilQ 280822..282537 (+) 1716 WP_175246732.1 type IV pilus secretin PilQ Machinery gene
  HPY05_RS01325 (HPY05_01325) aroK 282724..283248 (+) 525 WP_000818616.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37765.47 Da        Isoelectric Point: 7.1128

>NTDB_id=447685 HPY05_RS01300 WP_175246731.1 278115..279131(+) (pilM) [Vibrio cholerae strain W6G]
MGKSLVTGIDIGHHSIKAAVLKPMGDTYALVGYEELLVTADIFTDNHTLDYQKIVKKLKELKKGLPLFSHKVAIAIPDSA
VISKVLQIDSDLEQRELEFAVYQAFSHQSPFPVEELSLDFVKVTEKSLARSSTTTFQVYATKKDVVDSRLHAVKKAGFEP
VLMDVQVHSLLHLWQLASRAYRRPDWMLVDIGYTQSSLCLDFPEKMPFYKDVPLGTRQLESEPSAVNSLNHPLLHDPTQR
FIQELVDKIARQIQLFTSVHGTQSLGGLWLSGGGATLSGLEDELYQRLSLPCEVLNPFSLFKNNVAKRKRPLVDGQRFST
AAGLALRGLAWLESEHVA

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=447685 HPY05_RS01300 WP_175246731.1 278115..279131(+) (pilM) [Vibrio cholerae strain W6G]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCACCACAGCATTAAAGCTGCGGTGCTCAAACCTATGGGCGACAC
TTATGCGCTTGTGGGGTATGAGGAGTTGCTCGTTACCGCTGATATTTTCACCGATAATCACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTAAAAGAACTAAAAAAGGGTTTGCCTCTATTTAGTCATAAAGTCGCGATAGCAATCCCGGATAGCGCC
GTGATTAGCAAAGTATTACAAATAGATAGCGATCTTGAGCAGCGCGAGCTGGAATTTGCTGTCTATCAAGCCTTCTCGCA
TCAATCTCCATTCCCCGTTGAAGAGCTGAGCCTCGATTTCGTCAAAGTGACGGAAAAAAGCCTCGCCCGCAGCAGCACGA
CGACCTTCCAAGTGTATGCCACCAAAAAAGACGTGGTCGACAGTCGTTTGCATGCCGTTAAAAAAGCGGGTTTTGAACCT
GTGTTGATGGATGTGCAAGTACATAGTTTGTTGCATTTATGGCAACTCGCTAGCCGTGCGTATCGACGTCCAGACTGGAT
GTTGGTGGATATTGGCTACACCCAAAGTTCATTGTGCCTCGATTTTCCGGAAAAAATGCCCTTTTACAAAGACGTGCCAT
TAGGAACTCGTCAATTGGAATCAGAACCCAGTGCTGTGAACTCATTGAATCATCCACTGTTACACGATCCCACACAGCGG
TTTATTCAAGAATTGGTCGACAAAATTGCGCGGCAAATCCAGCTCTTTACTTCGGTGCACGGGACTCAAAGTCTTGGCGG
TTTATGGCTATCGGGTGGCGGTGCCACGCTTTCTGGTTTAGAGGACGAGCTTTATCAACGCTTGTCACTGCCTTGCGAAG
TGCTCAATCCGTTTTCACTGTTCAAAAACAATGTGGCAAAACGTAAACGCCCACTGGTTGATGGCCAGCGTTTTAGTACC
GCCGCCGGTCTTGCATTGCGAGGCTTAGCTTGGTTGGAGAGTGAGCATGTTGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(241-328)

Type IV pilus assembly protein PilM; (PilM_2)

(8-221)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

99.704

100

0.997
  pilM Vibrio campbellii strain DS40M4

48.817

100

0.488