Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   I5U72_RS12705 Genome accession   NZ_CP065417
Coordinates   2713380..2714051 (+) Length   223 a.a.
NCBI ID   WP_003090494.1    Uniprot ID   A0A0H2ZC88
Organism   Pseudomonas aeruginosa isolate P23     
Function   repress competence development (predicted from homology)   
Competence regulation

Genomic Context

Location: 2708380..2719051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I5U72_RS12670 (I5U72_12670) ppyR 2708853..2709110 (+) 258 WP_003090502.1 psl/pyoverdine operon transcriptional regulator PpyR -
  I5U72_RS12675 (I5U72_12675) - 2709097..2710290 (+) 1194 WP_003122598.1 NnrS family protein -
  I5U72_RS12680 (I5U72_12680) - 2710291..2710557 (-) 267 WP_003115326.1 DUF5329 domain-containing protein -
  I5U72_RS12685 (I5U72_12685) - 2710541..2711434 (-) 894 WP_003115327.1 hypothetical protein -
  I5U72_RS12690 (I5U72_12690) - 2711461..2712543 (-) 1083 WP_003113385.1 hypothetical protein -
  I5U72_RS12695 (I5U72_12695) - 2712758..2713066 (+) 309 WP_003090497.1 PepSY domain-containing protein -
  I5U72_RS12700 (I5U72_12700) - 2713066..2713380 (+) 315 WP_003097704.1 PepSY domain-containing protein -
  I5U72_RS12705 (I5U72_12705) ciaR 2713380..2714051 (+) 672 WP_003090494.1 two-component system response regulator CarR Regulator
  I5U72_RS12710 (I5U72_12710) carS 2714048..2715385 (+) 1338 WP_191519433.1 two-component system sensor histidine kinase CarS -
  I5U72_RS12715 (I5U72_12715) - 2715476..2715784 (+) 309 WP_003116713.1 hypothetical protein -
  I5U72_RS12720 (I5U72_12720) - 2715789..2717933 (-) 2145 WP_031755630.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 223 a.a.        Molecular weight: 24729.20 Da        Isoelectric Point: 5.8445

>NTDB_id=446894 I5U72_RS12705 WP_003090494.1 2713380..2714051(+) (ciaR) [Pseudomonas aeruginosa isolate P23]
MRLLLVEDHVPLADELMASLTRQGYAVDWLADGRDAAVQGASEPYDLIILDLGLPGRPGLEILQEWRGLGLATPVLILTA
RGSWAERIDGLKAGADDYLTKPFHPEELALRIQALLRRAHGLANQSQLEAAGLRLDEQRQCVCLNGADVDLTAAEFRLLR
YFMLHPGQVLSKGHLAEHLYDGETERDSNVIEVHVNHLRRKLGREVIETRRGQGYRYAGVAAG

Nucleotide


Download         Length: 672 bp        

>NTDB_id=446894 I5U72_RS12705 WP_003090494.1 2713380..2714051(+) (ciaR) [Pseudomonas aeruginosa isolate P23]
ATGCGGTTGCTGCTGGTTGAGGATCACGTTCCCCTGGCCGATGAACTGATGGCCAGCCTGACCCGCCAGGGCTACGCGGT
GGACTGGCTGGCCGACGGCCGCGACGCGGCGGTGCAGGGCGCCAGCGAGCCCTACGATCTGATCATCCTCGACCTCGGCC
TGCCCGGGCGTCCCGGCCTGGAGATACTCCAGGAGTGGCGTGGGCTGGGACTCGCGACTCCGGTGCTGATCCTCACCGCG
CGCGGTTCCTGGGCCGAACGCATCGATGGGCTCAAGGCCGGTGCCGACGACTACCTGACCAAGCCGTTCCATCCGGAAGA
GCTGGCCTTGCGCATCCAGGCCCTGTTGCGCCGCGCCCATGGGCTGGCCAACCAGAGCCAGCTGGAGGCCGCCGGGCTGC
GCCTGGACGAGCAGCGCCAGTGCGTGTGCCTGAACGGCGCCGACGTCGACCTGACCGCCGCCGAATTCCGCCTGCTGCGC
TATTTCATGCTGCACCCCGGCCAGGTCCTGTCCAAGGGGCACCTGGCCGAGCATCTGTACGACGGCGAGACCGAACGCGA
TTCCAACGTCATCGAGGTCCACGTCAACCACCTGCGGCGCAAGCTCGGCCGCGAGGTGATCGAGACCCGCCGTGGCCAGG
GCTACCGCTACGCCGGGGTGGCCGCCGGATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(147-216)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZC88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus mutans UA159

37.727

98.655

0.372
  ciaR Streptococcus pneumoniae Rx1

37.273

98.655

0.368
  ciaR Streptococcus pneumoniae D39

37.273

98.655

0.368
  ciaR Streptococcus pneumoniae R6

37.273

98.655

0.368
  ciaR Streptococcus pneumoniae TIGR4

37.273

98.655

0.368
  micA Streptococcus pneumoniae Cp1015

35.683

100

0.363
  vicR Streptococcus mutans UA159

35.526

100

0.363