Detailed information NTG0044629

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: BANAU_RS13360
• Genome accession: NC_017061
• Coordinates: 2748220..2748834 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis YAU B9601-Y2
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2743220..2753834

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BANAU_RS13335 (BANAU_2536)	rpsT	2743523..2743789 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
BANAU_RS13340 (BANAU_2537)	holA	2743806..2744847 (-)	1042	Protein_2644	DNA polymerase III subunit delta	-
BANAU_RS21765	-	2744887..2745038 (-)	152	Protein_2645	hypothetical protein	-
BANAU_RS21275 (BANAU_2538)	-	2745079..2745213 (+)	135	WP_003152870.1	YqzM family protein	-
BANAU_RS13350 (BANAU_2539)	comEC	2745232..2747583 (-)	2352	WP_014418418.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
BANAU_RS13355 (BANAU_2540)	-	2747584..2748153 (-)	570	WP_003152868.1	ComE operon protein 2	-
BANAU_RS13360 (BANAU_2541)	comEA	2748220..2748834 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
BANAU_RS13365 (BANAU_2542)	comER	2748893..2749714 (+)	822	WP_014418420.1	late competence protein ComER	-
BANAU_RS13370 (BANAU_2543)	-	2749783..2750520 (-)	738	WP_014418421.1	class I SAM-dependent DNA methyltransferase	-
BANAU_RS13375 (BANAU_2544)	rsfS	2750517..2750873 (-)	357	WP_007408260.1	ribosome silencing factor	-
BANAU_RS13380 (BANAU_2545)	yqeK	2750891..2751451 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
BANAU_RS13385 (BANAU_2546)	-	2751441..2752010 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
BANAU_RS13390 (BANAU_2547)	yhbY	2752021..2752311 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
BANAU_RS13395 (BANAU_2548)	aroE	2752305..2753147 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=44629 BANAU_RS13360 WP_014418419.1 2748220..2748834(-) (comEA) [Bacillus velezensis YAU B9601-Y2]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=44629 BANAU_RS13360 WP_014418419.1 2748220..2748834(-) (comEA) [Bacillus velezensis YAU B9601-Y2]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363