Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   I3J23_RS03265 Genome accession   NZ_CP065159
Coordinates   621178..622182 (-) Length   334 a.a.
NCBI ID   WP_003152685.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain GXU-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 616178..627182
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I3J23_RS03235 (I3J23_03235) - 617206..617862 (-) 657 WP_003152699.1 DUF421 domain-containing protein -
  I3J23_RS03240 (I3J23_03240) - 618029..618415 (+) 387 WP_003152697.1 TIGR04086 family membrane protein -
  I3J23_RS03245 (I3J23_03245) yajC 618467..618727 (-) 261 WP_003152695.1 preprotein translocase subunit YajC -
  I3J23_RS03250 (I3J23_03250) tgt 618758..619903 (-) 1146 WP_014305500.1 tRNA guanosine(34) transglycosylase Tgt -
  I3J23_RS03255 (I3J23_03255) queA 619931..620959 (-) 1029 WP_003152692.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  I3J23_RS03260 (I3J23_03260) - 620985..621185 (-) 201 WP_003152687.1 DUF2905 domain-containing protein -
  I3J23_RS03265 (I3J23_03265) ruvB 621178..622182 (-) 1005 WP_003152685.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  I3J23_RS03270 (I3J23_03270) ruvA 622193..622798 (-) 606 WP_003152683.1 Holliday junction branch migration protein RuvA Machinery gene
  I3J23_RS03275 (I3J23_03275) - 622933..623442 (-) 510 WP_046559922.1 intercompartmental signaling factor BofC -
  I3J23_RS03285 (I3J23_03285) - 623826..624419 (-) 594 WP_003152677.1 YhcN/YlaJ family sporulation lipoprotein -
  I3J23_RS03290 (I3J23_03290) safA 624567..625772 (-) 1206 WP_014305504.1 SafA/ExsA family spore coat assembly protein -
  I3J23_RS03295 (I3J23_03295) nadA 625899..627002 (-) 1104 WP_046559924.1 quinolinate synthase NadA -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37225.52 Da        Isoelectric Point: 4.7767

>NTDB_id=445754 I3J23_RS03265 WP_003152685.1 621178..622182(-) (ruvB) [Bacillus amyloliquefaciens strain GXU-1]
MDERLVSSEADSHESIIEQSLRPQNLAQYIGQKKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
MRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLNRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVLSRLEYYTQEELTDIVSRTADVFEVDIEEAPALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEEIAHDALERLQVDALGLDHIDHKLLMGMIEKFSGGPVGLDTISATIGEEPHTIEDVYEPYLLQIGFLQRTPRGRVVTP
AVYEHFRLEAPARD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=445754 I3J23_RS03265 WP_003152685.1 621178..622182(-) (ruvB) [Bacillus amyloliquefaciens strain GXU-1]
ATGGATGAACGGCTCGTTTCAAGTGAAGCAGACAGCCATGAATCAATAATCGAGCAAAGCCTCAGGCCGCAGAATCTCGC
CCAGTATATCGGCCAAAAGAAGGTGAAAGAAAATCTGCGCGTGTTTATCGATGCGGCAAAAATGAGGCAGGAAACGCTGG
ACCACGTTCTTTTATACGGGCCTCCGGGTCTCGGGAAAACAACGCTTGCGTCTATCGTGGCCAATGAGATGGGGGTGGAG
ATGCGGACGACGTCAGGTCCCGCCATCGAAAGACCGGGAGATTTGGCAGCCATCTTGACGGCGCTCGAACCAGGGGATGT
GCTGTTTATAGACGAAATTCACAGGCTGAACCGCTCGATTGAGGAAGTGCTGTACCCGGCGATGGAGGATTTCTGCCTTG
ATATCGTCATTGGAAAAGGCCCGTCCGCCCGCTCCGTCCGTCTCGACCTTCCCCCTTTTACGCTTGTAGGAGCGACGACA
AGGGTCGGATTATTGACCGCACCGCTCAGAGACCGGTTCGGGGTGCTGTCGCGCCTTGAATACTATACGCAGGAAGAGCT
GACAGATATCGTGTCAAGAACGGCTGACGTTTTTGAAGTGGATATCGAAGAAGCTCCGGCTCTGGAGATCGCCAGACGGT
CAAGGGGAACGCCGCGGGTTGCAAACCGGCTTCTCAGAAGGGTGAGAGATTTTGCCCAGGTGCTCGGCGACAGCCGCATC
ACGGAAGAAATCGCTCACGACGCGCTCGAACGTCTTCAAGTCGATGCGCTCGGCCTTGACCATATCGACCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAGCGGAGGTCCCGTCGGCCTTGATACGATTTCCGCGACAATCGGCGAAGAACCGCATA
CAATAGAGGATGTGTACGAACCGTATCTGCTGCAGATCGGATTTCTCCAGAGGACGCCGAGGGGACGGGTCGTGACTCCG
GCTGTGTATGAACATTTTCGATTGGAGGCGCCCGCCCGTGACTGA

Domains

Predicted by InterProScan.

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB AAA lid domain (AAA_lid_4)

(182-255)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

91.916

100

0.919
  ruvB Streptococcus pneumoniae TIGR4

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae R6

60.736

97.605

0.593
  ruvB Streptococcus pneumoniae D39

60.736

97.605

0.593
  ruvB Synechocystis sp. PCC 6803

58.359

98.503

0.575
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518