NTDB

Overview

Name	ruvA	Type	Machinery gene
Locus tag	I3J23_RS03270	Genome accession	NZ_CP065159
Coordinates	622193..622798 (-)	Length	201 a.a.
NCBI ID	WP_003152683.1	Uniprot ID	A7Z769
Organism	Bacillus amyloliquefaciens strain GXU-1
Function	homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 617193..627798

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
I3J23_RS03235 (I3J23_03235)	-	617206..617862 (-)	657	WP_003152699.1	DUF421 domain-containing protein	-
I3J23_RS03240 (I3J23_03240)	-	618029..618415 (+)	387	WP_003152697.1	TIGR04086 family membrane protein	-
I3J23_RS03245 (I3J23_03245)	yajC	618467..618727 (-)	261	WP_003152695.1	preprotein translocase subunit YajC	-
I3J23_RS03250 (I3J23_03250)	tgt	618758..619903 (-)	1146	WP_014305500.1	tRNA guanosine(34) transglycosylase Tgt	-
I3J23_RS03255 (I3J23_03255)	queA	619931..620959 (-)	1029	WP_003152692.1	tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA	-
I3J23_RS03260 (I3J23_03260)	-	620985..621185 (-)	201	WP_003152687.1	DUF2905 domain-containing protein	-
I3J23_RS03265 (I3J23_03265)	ruvB	621178..622182 (-)	1005	WP_003152685.1	Holliday junction branch migration DNA helicase RuvB	Machinery gene
I3J23_RS03270 (I3J23_03270)	ruvA	622193..622798 (-)	606	WP_003152683.1	Holliday junction branch migration protein RuvA	Machinery gene
I3J23_RS03275 (I3J23_03275)	-	622933..623442 (-)	510	WP_046559922.1	intercompartmental signaling factor BofC	-
I3J23_RS03285 (I3J23_03285)	-	623826..624419 (-)	594	WP_003152677.1	YhcN/YlaJ family sporulation lipoprotein	-
I3J23_RS03290 (I3J23_03290)	safA	624567..625772 (-)	1206	WP_014305504.1	SafA/ExsA family spore coat assembly protein	-
I3J23_RS03295 (I3J23_03295)	nadA	625899..627002 (-)	1104	WP_046559924.1	quinolinate synthase NadA	-

Sequence

Protein

Download Length: 201 a.a. Molecular weight: 22414.85 Da Isoelectric Point: 5.1597

                                >NTDB_id=445755 I3J23_RS03270 WP_003152683.1 622193..622798(-) (ruvA) [Bacillus amyloliquefaciens strain GXU-1]

MIEFVKGTIDYVSPQYIVIENGGIGYQVFTPNPFIYKVSNQETIFTYHHIKEDAFSLYGFSTREEKALFTKLLNVTGIGP

KGALAILGSGDPGAVIEAIEQEDEAFLVKFPGVGKKTARQIILDLKGKLADVVPEMIDNLFNHEARIEKQEAETALDEAL

EALRVLGYAEKEIKKVLPHLKEETALSTDQYVKKALQKLLK

Nucleotide

Download Length: 606 bp

                                >NTDB_id=445755 I3J23_RS03270 WP_003152683.1 622193..622798(-) (ruvA) [Bacillus amyloliquefaciens strain GXU-1]

GTGATTGAATTCGTGAAGGGGACGATTGATTATGTATCTCCCCAATACATCGTGATTGAAAACGGCGGAATCGGCTATCA

GGTCTTTACTCCGAATCCGTTTATTTATAAGGTAAGCAATCAAGAAACCATTTTTACGTATCATCATATAAAAGAAGATG

CCTTTTCTTTGTACGGTTTTTCAACCCGGGAAGAAAAAGCGCTTTTCACAAAGCTGCTGAACGTCACCGGCATCGGGCCG

AAAGGGGCGCTCGCCATCCTCGGTTCCGGTGACCCCGGAGCCGTCATCGAAGCGATCGAACAGGAAGATGAAGCCTTTTT

GGTGAAATTTCCGGGTGTGGGCAAAAAAACGGCGCGGCAGATTATTCTTGATTTGAAAGGCAAACTGGCCGACGTCGTGC

CTGAAATGATTGACAACCTGTTCAATCATGAAGCGCGCATTGAAAAACAAGAGGCTGAAACGGCGCTTGACGAGGCGCTT

GAAGCTCTTAGGGTTCTCGGCTACGCCGAAAAAGAAATTAAAAAAGTGCTTCCTCACTTAAAAGAGGAAACGGCGCTTTC

AACAGATCAATATGTGAAAAAAGCATTGCAAAAATTATTAAAGTAA

Domains

Predicted by InterProScan.

RuvA, C-terminal domain (RuvA_C)

(155-199)

RuvA N terminal domain (RuvA_N)

(1-59)

Helix-hairpin-helix domain (HHH_5)

(69-126)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A7Z769

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ruvA	Bacillus subtilis subsp. subtilis str. 168	92.537	100	0.925
ruvA	Streptococcus pneumoniae R6	39.806	100	0.408
ruvA	Streptococcus pneumoniae D39	39.806	100	0.408
ruvA	Streptococcus pneumoniae TIGR4	39.806	100	0.408

Detailed information NTG0445755