Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   I2T83_RS02245 Genome accession   NZ_CP065136
Coordinates   483177..484301 (+) Length   374 a.a.
NCBI ID   WP_001521436.1    Uniprot ID   -
Organism   Escherichia coli strain EE72d     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 478177..489301
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  I2T83_RS02220 (I2T83_02220) mscL 478303..478713 (-) 411 WP_000022442.1 large-conductance mechanosensitive channel protein MscL -
  I2T83_RS02225 (I2T83_02225) trkA 478843..480219 (-) 1377 WP_000691382.1 Trk system potassium transporter TrkA -
  I2T83_RS02230 (I2T83_02230) rsmB 480241..481530 (-) 1290 WP_001308993.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  I2T83_RS02235 (I2T83_02235) fmt 481576..482523 (-) 948 WP_000004456.1 methionyl-tRNA formyltransferase -
  I2T83_RS02240 (I2T83_02240) def 482538..483047 (-) 510 WP_000114984.1 peptide deformylase -
  I2T83_RS02245 (I2T83_02245) dprA 483177..484301 (+) 1125 WP_001521436.1 DNA-protecting protein DprA Machinery gene
  I2T83_RS02250 (I2T83_02250) smg 484273..484746 (+) 474 WP_000460672.1 DUF494 family protein Smg -
  I2T83_RS02255 (I2T83_02255) yrdD 484775..485317 (+) 543 WP_001129723.1 type I DNA topoisomerase -
  I2T83_RS02260 (I2T83_02260) tsaC 485322..485894 (+) 573 WP_001297709.1 L-threonylcarbamoyladenylate synthase type 1 TsaC -
  I2T83_RS02265 (I2T83_02265) aroE 485899..486717 (+) 819 WP_001521434.1 shikimate dehydrogenase -
  I2T83_RS02270 (I2T83_02270) yrdB 486714..486971 (+) 258 WP_001521433.1 DUF1488 domain-containing protein -
  I2T83_RS02275 (I2T83_02275) yrdA 486947..487501 (-) 555 WP_001286217.1 gamma carbonic anhydrase family protein -

Sequence

Protein


Download         Length: 374 a.a.        Molecular weight: 40994.94 Da        Isoelectric Point: 6.2655

>NTDB_id=445566 I2T83_RS02245 WP_001521436.1 483177..484301(+) (dprA) [Escherichia coli strain EE72d]
MVDTEIWLRLMSVSSLYGDDMVRIAHWLAKQSHIDAVVLQQTGLTLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSE
FYPPQLLATTDYPGALFVEGELHALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGLSKGVLVVEAALRSGSLVTARCA
LEQGREVFALPGPLGNPGSEGPHWLIKQGAILVTEPEEILENLQFGLHWLPDAPENSFYSPDQEDVALPFPELLANVGDE
VTPVDVVAERAGQPVPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV

Nucleotide


Download         Length: 1125 bp        

>NTDB_id=445566 I2T83_RS02245 WP_001521436.1 483177..484301(+) (dprA) [Escherichia coli strain EE72d]
ATGGTCGATACAGAAATTTGGCTGCGTTTAATGAGCGTCAGCAGCTTGTACGGCGATGATATGGTCCGTATAGCTCACTG
GCTGGCAAAACAGTCGCATATTGATGCGGTTGTATTGCAGCAAACAGGGCTTACATTGCGGCAGGCACAACGCTTTCTTT
CATTTCCGCGAAAGAGTATCGAAAGCTCACTTTGTTGGTTGGAGCAACCCAACCATCATTTAATCCCTGCGGACAGCGAA
TTTTATCCTCCTCAACTTCTTGCGACGACAGATTACCCCGGCGCACTGTTTGTTGAAGGAGAACTGCACGCGCTGCATTC
ATTTCAGCTTGCCGTAGTGGGGAGTCGGGCGCATTCATGGTATGGCGAGCGATGGGGACGATTATTTTGCGAAACTCTGG
CGACGCGTGGAGTGACAATTACGAGTGGACTGGCGCGTGGAATCGATGGTGTGGCGCATAAAGCGGCCTTACAGGTAAAT
GGCGTCAGCATTGCTGTATTAGGGAATGGACTTAATACCATTCATCCGCGTCGCCATGCCCGACTGGCTACCAGTTTGCT
TGAACATGGTGGGGCTCTTGTCTCGGAATTTCCCCTCGATGTTCCTCCCCTTGCTTACAATTTCCCACGAAGAAATCGCA
TTATCAGTGGTCTAAGTAAAGGTGTACTGGTGGTGGAAGCGGCTTTGCGCAGTGGTTCGCTGGTGACAGCACGTTGTGCG
CTTGAGCAGGGGCGTGAAGTTTTTGCCTTGCCGGGTCCATTAGGGAATCCGGGAAGCGAAGGGCCTCACTGGTTAATAAA
ACAAGGTGCGATTCTTGTGACGGAACCGGAAGAAATTCTGGAAAACTTGCAATTTGGATTGCACTGGTTGCCAGACGCCC
CTGAAAATTCATTTTATTCACCAGATCAGGAAGACGTGGCATTGCCATTTCCTGAGCTCCTGGCTAACGTAGGAGATGAG
GTAACACCTGTTGACGTCGTCGCTGAACGTGCCGGCCAACCTGTGCCAGAGGTAGTAACTCAACTACTCGAACTGGAGTT
AGCAGGATGGATCGCAGCTGTACCCGGCGGCTATGTCCGATTGAGGAGGGCATGCCATGTTCGACGTACTAATGTATTTG
TTTGA

Domains

Predicted by InterProScan.

DprA winged helix domain (WHD_DprA)

(312-357)

DNA recombination-mediator protein A (DNA_processg_A)

(67-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio campbellii strain DS40M4

48.509

98.663

0.479
  dprA Vibrio cholerae O1 biovar El Tor strain E7946

48.71

82.888

0.404
  dprA Vibrio cholerae strain A1552

48.71

82.888

0.404
  dprA Glaesserella parasuis strain SC1401

44.807

90.107

0.404
  dprA Haemophilus influenzae Rd KW20

44.214

90.107

0.398
  dprA Legionella pneumophila strain ERS1305867

43.939

88.235

0.388