Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   HO396_RS17990 Genome accession   NZ_CP053602
Coordinates   3714104..3714589 (+) Length   161 a.a.
NCBI ID   WP_000157061.1    Uniprot ID   A0AAU7IP99
Organism   Escherichia coli BL21(DE3)     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 3705391..3723941 3714104..3714589 within 0


Gene organization within MGE regions


Location: 3705391..3723941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HO396_RS17965 (HO396_17970) - 3705391..3706575 (+) 1185 WP_001218910.1 integrase arm-type DNA-binding domain-containing protein -
  HO396_RS17970 (HO396_17975) - 3706986..3708029 (+) 1044 WP_000999851.1 hypothetical protein -
  HO396_RS17975 (HO396_17980) - 3708427..3709497 (-) 1071 WP_000412404.1 LacI family DNA-binding transcriptional regulator -
  HO396_RS17980 (HO396_17985) - 3709759..3711060 (+) 1302 WP_001329026.1 MFS transporter -
  HO396_RS17985 (HO396_17990) - 3711075..3713456 (+) 2382 WP_001313395.1 glycoside hydrolase family 31 protein -
  HO396_RS22445 ykgR 3713637..3713738 (+) 102 WP_001313396.1 small membrane protein YkgR -
  HO396_RS17990 (HO396_17995) ssb 3714104..3714589 (+) 486 WP_000157061.1 single-stranded DNA-binding protein Machinery gene
  HO396_RS18000 (HO396_18005) - 3715654..3716000 (-) 347 Protein_3557 transposase -
  HO396_RS18005 (HO396_18010) - 3716036..3716491 (-) 456 Protein_3558 hypothetical protein -
  HO396_RS18010 (HO396_18015) - 3716540..3716695 (-) 156 WP_000074142.1 hypothetical protein -
  HO396_RS18015 (HO396_18020) - 3717315..3717695 (+) 381 Protein_3560 integrase core domain-containing protein -
  HO396_RS18020 (HO396_18025) - 3717974..3721471 (+) 3498 WP_001038839.1 autotransporter outer membrane beta-barrel domain-containing protein -
  HO396_RS18025 (HO396_18030) - 3721646..3721851 (-) 206 Protein_3562 hypothetical protein -
  HO396_RS22285 - 3721850..3721963 (+) 114 Protein_3563 alkaline phosphatase -
  HO396_RS22290 - 3722331..3722525 (-) 195 Protein_3564 hypothetical protein -
  HO396_RS18035 (HO396_18040) - 3722504..3722749 (+) 246 Protein_3565 transposase -
  HO396_RS18040 (HO396_18045) - 3722817..3723863 (+) 1047 Protein_3566 IS30-like element IS30 family transposase -

Sequence


Protein


Download         Length: 161 a.a.        Molecular weight: 17685.62 Da        Isoelectric Point: 7.2017

>NTDB_id=445443 HO396_RS17990 WP_000157061.1 3714104..3714589(+) (ssb) [Escherichia coli BL21(DE3)]
MASKGINKVILIGYLGQDPDIRYMPNGGAVASIALATSETWRDKQTGEMREQTEWHRVVLFGKLAEVASEYLRKGAQVYI
DGQLRTRKWTDQTGQERYTTEVVVHTGGTMQMLGSRQNSAQGGRGQPQQPQQGRQFSGNSPSPGNTAMSGSFSDDDDSIP
F

Nucleotide


Download         Length: 486 bp        

>NTDB_id=445443 HO396_RS17990 WP_000157061.1 3714104..3714589(+) (ssb) [Escherichia coli BL21(DE3)]
ATGGCCTCTAAAGGTATTAACAAAGTTATTCTGATAGGGTATCTGGGTCAGGACCCGGATATTCGCTATATGCCAAACGG
CGGCGCTGTGGCCAGCATTGCGCTGGCCACCTCCGAAACCTGGCGGGACAAACAGACAGGGGAGATGCGGGAACAAACCG
AATGGCACCGCGTGGTGCTGTTCGGCAAACTGGCGGAAGTAGCCAGTGAATACCTGCGCAAAGGAGCTCAGGTGTACATC
GATGGTCAGCTGCGTACCCGAAAATGGACCGACCAGACCGGCCAGGAACGCTACACGACTGAAGTGGTGGTGCATACCGG
CGGTACCATGCAGATGCTGGGCTCCCGGCAGAATAGTGCACAGGGTGGCCGGGGACAGCCTCAACAACCACAACAGGGCA
GACAGTTTTCAGGTAATTCGCCATCTCCGGGCAATACCGCCATGTCCGGCAGTTTCAGCGACGATGACGACAGTATCCCG
TTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

58.757

100

0.646

  ssb Glaesserella parasuis strain SC1401

52.632

94.41

0.497

  ssb Neisseria meningitidis MC58

41.477

100

0.453

  ssb Neisseria gonorrhoeae MS11

41.477

100

0.453

  ssb Latilactobacillus sakei subsp. sakei 23K

32.402

100

0.36