Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   HO403_RS03500 Genome accession   NZ_CP053592
Coordinates   744448..744933 (-) Length   161 a.a.
NCBI ID   WP_000157061.1    Uniprot ID   A0AAU7IP99
Organism   Escherichia coli strain NEBExpress_Iq     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 736177..753646 744448..744933 within 0


Gene organization within MGE regions


Location: 736177..753646
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HO403_RS03455 (HO403_03455) - 736288..736533 (-) 246 Protein_668 transposase -
  HO403_RS21840 - 736512..736706 (+) 195 Protein_669 hypothetical protein -
  HO403_RS21845 - 737074..737187 (-) 114 Protein_670 alkaline phosphatase -
  HO403_RS03465 (HO403_03465) - 737186..737391 (+) 206 Protein_671 hypothetical protein -
  HO403_RS03470 (HO403_03470) - 737566..741063 (-) 3498 WP_001038839.1 autotransporter outer membrane beta-barrel domain-containing protein -
  HO403_RS03475 (HO403_03475) - 741342..741722 (-) 381 Protein_673 integrase core domain-containing protein -
  HO403_RS03480 (HO403_03480) - 742342..742497 (+) 156 WP_000074142.1 hypothetical protein -
  HO403_RS03485 (HO403_03485) - 742546..743001 (+) 456 Protein_675 hypothetical protein -
  HO403_RS03490 (HO403_03490) - 743037..743383 (+) 347 Protein_676 transposase -
  HO403_RS03500 (HO403_03500) ssb 744448..744933 (-) 486 WP_000157061.1 single-stranded DNA-binding protein Machinery gene
  HO403_RS22075 ykgR 745299..745400 (-) 102 WP_001313396.1 small membrane protein YkgR -
  HO403_RS03505 (HO403_03505) - 745581..747962 (-) 2382 WP_001313395.1 glycoside hydrolase family 31 protein -
  HO403_RS03510 (HO403_03510) - 747977..749278 (-) 1302 WP_001329026.1 MFS transporter -
  HO403_RS03515 (HO403_03515) - 749540..750610 (+) 1071 WP_000412404.1 LacI family DNA-binding transcriptional regulator -
  HO403_RS03520 (HO403_03520) - 751008..752051 (-) 1044 WP_000999851.1 hypothetical protein -
  HO403_RS03525 (HO403_03525) - 752462..753646 (-) 1185 WP_001218910.1 integrase arm-type DNA-binding domain-containing protein -

Sequence


Protein


Download         Length: 161 a.a.        Molecular weight: 17685.62 Da        Isoelectric Point: 7.2017

>NTDB_id=445302 HO403_RS03500 WP_000157061.1 744448..744933(-) (ssb) [Escherichia coli strain NEBExpress_Iq]
MASKGINKVILIGYLGQDPDIRYMPNGGAVASIALATSETWRDKQTGEMREQTEWHRVVLFGKLAEVASEYLRKGAQVYI
DGQLRTRKWTDQTGQERYTTEVVVHTGGTMQMLGSRQNSAQGGRGQPQQPQQGRQFSGNSPSPGNTAMSGSFSDDDDSIP
F

Nucleotide


Download         Length: 486 bp        

>NTDB_id=445302 HO403_RS03500 WP_000157061.1 744448..744933(-) (ssb) [Escherichia coli strain NEBExpress_Iq]
ATGGCCTCTAAAGGTATTAACAAAGTTATTCTGATAGGGTATCTGGGTCAGGACCCGGATATTCGCTATATGCCAAACGG
CGGCGCTGTGGCCAGCATTGCGCTGGCCACCTCCGAAACCTGGCGGGACAAACAGACAGGGGAGATGCGGGAACAAACCG
AATGGCACCGCGTGGTGCTGTTCGGCAAACTGGCGGAAGTAGCCAGTGAATACCTGCGCAAAGGAGCTCAGGTGTACATC
GATGGTCAGCTGCGTACCCGAAAATGGACCGACCAGACCGGCCAGGAACGCTACACGACTGAAGTGGTGGTGCATACCGG
CGGTACCATGCAGATGCTGGGCTCCCGGCAGAATAGTGCACAGGGTGGCCGGGGACAGCCTCAACAACCACAACAGGGCA
GACAGTTTTCAGGTAATTCGCCATCTCCGGGCAATACCGCCATGTCCGGCAGTTTCAGCGACGATGACGACAGTATCCCG
TTCTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

58.757

100

0.646

  ssb Glaesserella parasuis strain SC1401

52.632

94.41

0.497

  ssb Neisseria meningitidis MC58

41.477

100

0.453

  ssb Neisseria gonorrhoeae MS11

41.477

100

0.453

  ssb Latilactobacillus sakei subsp. sakei 23K

32.402

100

0.36