Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HOO69_RS12780 Genome accession   NZ_CP053541
Coordinates   2771538..2772125 (-) Length   195 a.a.
NCBI ID   WP_171802178.1    Uniprot ID   A0AAE7DXD5
Organism   Vibrio europaeus strain NPI-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2766538..2777125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HOO69_RS12760 (HOO69_12800) aroB 2767525..2768613 (-) 1089 WP_171383619.1 3-dehydroquinate synthase -
  HOO69_RS12765 (HOO69_12805) aroK 2768642..2769160 (-) 519 WP_069667329.1 shikimate kinase AroK -
  HOO69_RS12770 (HOO69_12810) pilQ 2769337..2771013 (-) 1677 WP_171802406.1 type IV pilus secretin PilQ Machinery gene
  HOO69_RS12775 (HOO69_12815) pilP 2771045..2771548 (-) 504 WP_171802177.1 pilus assembly protein PilP Machinery gene
  HOO69_RS12780 (HOO69_12820) pilO 2771538..2772125 (-) 588 WP_171802178.1 type 4a pilus biogenesis protein PilO Machinery gene
  HOO69_RS12785 (HOO69_12825) pilN 2772112..2772675 (-) 564 WP_171802179.1 PilN domain-containing protein Machinery gene
  HOO69_RS12790 (HOO69_12830) pilM 2772665..2773660 (-) 996 WP_069667325.1 type IV pilus assembly protein PilM Machinery gene
  HOO69_RS12795 (HOO69_12835) - 2773801..2776320 (+) 2520 WP_171383617.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 195 a.a.        Molecular weight: 22555.02 Da        Isoelectric Point: 9.0481

>NTDB_id=445063 HOO69_RS12780 WP_171802178.1 2771538..2772125(-) (pilO) [Vibrio europaeus strain NPI-1]
MYRFNLDIEEIGEWPLTYQLVVMAVLMAILQGGGYWLFAQPKLGQLDLLKQQEQQHKTSIALKTKKVVLLPRIKAQLDKL
SQRYDYLSRQLPAQKELASMLASVNQVGLQNSLTFTRIDWGHKHNQAFLYKLPLNIELTGDYHDIGDFSAAIASLPRIIN
FDDVYWHRVSLESSTLHFRVRAYTYQLKGEVKDES

Nucleotide


Download         Length: 588 bp        

>NTDB_id=445063 HOO69_RS12780 WP_171802178.1 2771538..2772125(-) (pilO) [Vibrio europaeus strain NPI-1]
GTGTATCGCTTTAACCTTGATATTGAAGAGATAGGCGAGTGGCCACTTACTTATCAGCTAGTCGTGATGGCCGTGTTGAT
GGCGATTTTACAGGGAGGCGGTTATTGGTTATTTGCTCAGCCTAAGCTTGGACAACTCGATCTTCTTAAACAGCAAGAGC
AGCAGCACAAAACCTCAATAGCATTGAAAACGAAAAAAGTGGTGTTGTTACCTAGAATAAAGGCCCAGCTTGATAAACTG
TCACAGCGTTATGATTACCTATCGCGTCAATTGCCCGCACAAAAAGAGCTTGCTTCTATGTTGGCGTCGGTTAATCAGGT
CGGCTTACAGAATTCCCTGACCTTTACCCGAATTGATTGGGGACACAAACACAACCAAGCGTTTCTATACAAGCTGCCAC
TCAACATTGAATTGACTGGCGATTATCACGATATCGGTGATTTTTCTGCAGCAATTGCCTCGCTACCGCGAATTATAAAT
TTTGATGATGTCTATTGGCATCGTGTCAGCTTAGAAAGCAGCACACTGCATTTTCGGGTCCGAGCTTACACCTATCAGCT
TAAAGGTGAGGTCAAAGATGAAAGTTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

66.842

97.436

0.651
  pilO Vibrio campbellii strain DS40M4

58.696

94.359

0.554