Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   HOO69_RS12785 Genome accession   NZ_CP053541
Coordinates   2772112..2772675 (-) Length   187 a.a.
NCBI ID   WP_171802179.1    Uniprot ID   -
Organism   Vibrio europaeus strain NPI-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2767112..2777675
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HOO69_RS12760 (HOO69_12800) aroB 2767525..2768613 (-) 1089 WP_171383619.1 3-dehydroquinate synthase -
  HOO69_RS12765 (HOO69_12805) aroK 2768642..2769160 (-) 519 WP_069667329.1 shikimate kinase AroK -
  HOO69_RS12770 (HOO69_12810) pilQ 2769337..2771013 (-) 1677 WP_171802406.1 type IV pilus secretin PilQ Machinery gene
  HOO69_RS12775 (HOO69_12815) pilP 2771045..2771548 (-) 504 WP_171802177.1 pilus assembly protein PilP Machinery gene
  HOO69_RS12780 (HOO69_12820) pilO 2771538..2772125 (-) 588 WP_171802178.1 type 4a pilus biogenesis protein PilO Machinery gene
  HOO69_RS12785 (HOO69_12825) pilN 2772112..2772675 (-) 564 WP_171802179.1 PilN domain-containing protein Machinery gene
  HOO69_RS12790 (HOO69_12830) pilM 2772665..2773660 (-) 996 WP_069667325.1 type IV pilus assembly protein PilM Machinery gene
  HOO69_RS12795 (HOO69_12835) - 2773801..2776320 (+) 2520 WP_171383617.1 penicillin-binding protein 1A -
  HOO69_RS12800 (HOO69_12840) oxyR 2776383..2777270 (-) 888 WP_230300123.1 DNA-binding transcriptional regulator OxyR -
  HOO69_RS25190 - 2777226..2777413 (+) 188 Protein_2467 hypothetical protein -

Sequence

Protein


Download         Length: 187 a.a.        Molecular weight: 21537.81 Da        Isoelectric Point: 7.4627

>NTDB_id=445064 HOO69_RS12785 WP_171802179.1 2772112..2772675(-) (pilN) [Vibrio europaeus strain NPI-1]
MLHNINLLPWREELRVYRQKRFGVLMMVTLIIAVVIQWGIGSYIEQQQERQSERNNYLVRYDTQLDQQLNQLKIVEQEHQ
ALLTRLSVIEDLQKERNKTTDFMNLLPELIPPGVYIDKLKINGLQVELAGISDSTSHLASMLDNLEKSPAITNVQMHSIV
HGTARFASKYQSYRVSFSLLGGQSVSL

Nucleotide


Download         Length: 564 bp        

>NTDB_id=445064 HOO69_RS12785 WP_171802179.1 2772112..2772675(-) (pilN) [Vibrio europaeus strain NPI-1]
ATGCTGCATAACATCAATTTGTTGCCGTGGCGTGAAGAGCTGCGTGTTTATCGCCAAAAGCGCTTTGGAGTGCTGATGAT
GGTTACGTTAATCATTGCTGTGGTTATCCAGTGGGGCATTGGCAGTTATATAGAGCAGCAACAGGAACGGCAATCTGAAC
GTAATAACTATCTTGTGCGTTATGACACACAGTTGGATCAACAGCTTAACCAACTGAAAATCGTCGAACAAGAACATCAA
GCCCTATTGACCCGCTTGTCGGTTATTGAGGATTTGCAGAAAGAGCGCAATAAAACCACAGACTTTATGAATTTATTGCC
TGAGCTGATTCCGCCTGGTGTTTACATCGACAAGTTAAAGATCAATGGCCTACAGGTTGAGCTTGCTGGGATCAGCGATT
CAACTTCTCATCTTGCCTCTATGCTAGATAACCTCGAAAAATCACCCGCTATAACTAACGTCCAGATGCATTCCATTGTT
CATGGTACGGCTCGATTTGCCAGTAAGTACCAATCATATCGCGTCTCTTTTTCTCTTCTAGGAGGTCAGAGTGTATCGCT
TTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio cholerae strain A1552

53.889

96.257

0.519
  pilN Vibrio campbellii strain DS40M4

51.064

100

0.513