Detailed information NTG0445065

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HOO69_RS12790
• Genome accession: NZ_CP053541
• Coordinates: 2772665..2773660 (-)
• Length: 331 a.a.
• NCBI ID: WP_069667325.1
• Uniprot ID: A0A178JAY1
• Organism: Vibrio europaeus strain NPI-1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2767665..2778660

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HOO69_RS12765 (HOO69_12805)	aroK	2768642..2769160 (-)	519	WP_069667329.1	shikimate kinase AroK	-
HOO69_RS12770 (HOO69_12810)	pilQ	2769337..2771013 (-)	1677	WP_171802406.1	type IV pilus secretin PilQ	Machinery gene
HOO69_RS12775 (HOO69_12815)	pilP	2771045..2771548 (-)	504	WP_171802177.1	pilus assembly protein PilP	Machinery gene
HOO69_RS12780 (HOO69_12820)	pilO	2771538..2772125 (-)	588	WP_171802178.1	type 4a pilus biogenesis protein PilO	Machinery gene
HOO69_RS12785 (HOO69_12825)	pilN	2772112..2772675 (-)	564	WP_171802179.1	PilN domain-containing protein	Machinery gene
HOO69_RS12790 (HOO69_12830)	pilM	2772665..2773660 (-)	996	WP_069667325.1	type IV pilus assembly protein PilM	Machinery gene
HOO69_RS12795 (HOO69_12835)	-	2773801..2776320 (+)	2520	WP_171383617.1	penicillin-binding protein 1A	-
HOO69_RS12800 (HOO69_12840)	oxyR	2776383..2777270 (-)	888	WP_230300123.1	DNA-binding transcriptional regulator OxyR	-
HOO69_RS25190	-	2777226..2777413 (+)	188	Protein_2467	hypothetical protein	-
HOO69_RS12805 (HOO69_12845)	-	2777449..2778180 (+)	732	WP_069667322.1	glutathione peroxidase	-
HOO69_RS12810 (HOO69_12850)	-	2778252..2778599 (-)	348	WP_004743292.1	RidA family protein	-

Sequence

Protein

• Length: 331 a.a.
• Molecular weight: 36405.82 Da
• Isoelectric Point: 7.2852

>NTDB_id=445065 HOO69_RS12790 WP_069667325.1 2772665..2773660(-) (pilM) [Vibrio europaeus strain NPI-1]
MGKSLVTGIEIGHHSIKAVVLKPNKGTVALVDYQELPIEAGIFSDNRMSNYQKIVKKLKELRKSLPRFSTKVALAIPDNA
VISKVLQIDSGLSDNEIEYAISDAFSHQTPFPIDDLYLDYVPLTSNGQSSPTHYQVYATKKELVAPRVEVFRSASFQPVV
VDLRAHALVHIWQLAARQQVKTDWLLIDVGFDHASLCMDLADKAPFCKEIVLATHEKEADSNHRLSLAQSREGFSAELLD
KIQRNLQLIVSMHGKSVNGIWLSGVGAAMPVLANEISTRLAIECELLNPLTLFSTTSQQPSANLLASGHRFTSAAGIALR
ALTWLESRNAA

Nucleotide

• Length: 996 bp

>NTDB_id=445065 HOO69_RS12790 WP_069667325.1 2772665..2773660(-) (pilM) [Vibrio europaeus strain NPI-1]
ATGGGTAAATCATTAGTTACAGGCATTGAAATTGGACACCACAGTATTAAAGCGGTGGTTCTCAAGCCAAACAAGGGCAC
AGTTGCTCTTGTCGACTATCAAGAATTGCCAATCGAAGCGGGCATTTTCTCTGATAACCGTATGTCAAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGGAAGAGTTTGCCACGATTTAGTACGAAAGTTGCATTAGCGATACCAGATAATGCG
GTAATAAGTAAGGTATTACAGATAGATAGCGGGCTTAGCGACAATGAAATTGAGTACGCTATTTCCGATGCATTTTCCCA
TCAAACTCCGTTTCCAATTGACGATCTCTACTTAGATTATGTTCCCCTGACCAGTAATGGGCAGTCATCGCCAACTCACT
ACCAAGTATACGCGACGAAAAAAGAGCTAGTTGCCCCTAGAGTTGAAGTATTCAGAAGCGCTTCCTTTCAACCCGTAGTG
GTTGATTTACGTGCTCATGCTTTAGTGCATATTTGGCAGCTTGCTGCACGACAGCAGGTGAAAACCGATTGGCTTCTGAT
AGATGTTGGTTTTGACCACGCTTCGTTGTGTATGGACCTTGCTGATAAAGCGCCTTTTTGCAAAGAGATCGTTTTGGCAA
CCCATGAAAAAGAGGCGGATTCAAACCATCGCTTAAGCCTGGCGCAAAGCCGCGAAGGGTTCAGCGCCGAGTTACTAGAT
AAAATCCAACGCAATCTACAGCTTATTGTTTCTATGCACGGTAAGAGTGTTAACGGGATTTGGCTGTCAGGTGTTGGGGC
TGCCATGCCTGTTCTGGCGAATGAAATCAGCACGCGCCTTGCCATTGAATGTGAGCTTCTCAACCCGCTTACTCTTTTTT
CAACAACGAGCCAACAGCCATCCGCAAATCTGTTGGCTAGTGGGCATAGGTTCACATCGGCGGCTGGGATTGCTCTGCGC
GCGCTCACTTGGTTGGAGAGTCGCAATGCTGCATAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-195)

• Type IV pilus assembly protein PilM; (PilM_2) (234-321)

3D structure

• AlphaFold DB: A0A178JAY1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio cholerae strain A1552	51.765	100	0.532
pilM	Vibrio campbellii strain DS40M4	41.964	100	0.426