Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   ITP52_RS19305 Genome accession   NZ_CP064818
Coordinates   3786893..3787510 (+) Length   205 a.a.
NCBI ID   WP_041850029.1    Uniprot ID   -
Organism   Bacillus subtilis strain SH1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3781893..3792510
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ITP52_RS19270 (ITP52_19270) aroE 3782551..3783393 (+) 843 WP_041850031.1 shikimate dehydrogenase -
  ITP52_RS19275 (ITP52_19275) yhbY 3783387..3783677 (+) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  ITP52_RS19280 (ITP52_19280) nadD 3783689..3784258 (+) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  ITP52_RS19285 (ITP52_19285) yqeK 3784248..3784808 (+) 561 WP_014480316.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  ITP52_RS19290 (ITP52_19290) rsfS 3784826..3785182 (+) 357 WP_003237112.1 ribosome silencing factor -
  ITP52_RS19295 (ITP52_19295) yqeM 3785179..3785922 (+) 744 WP_032726197.1 class I SAM-dependent methyltransferase -
  ITP52_RS19300 (ITP52_19300) comER 3785988..3786809 (-) 822 WP_041850030.1 late competence protein ComER -
  ITP52_RS19305 (ITP52_19305) comEA 3786893..3787510 (+) 618 WP_041850029.1 competence protein ComEA Machinery gene
  ITP52_RS19310 (ITP52_19310) comEB 3787577..3788146 (+) 570 WP_003229978.1 ComE operon protein 2 -
  ITP52_RS19315 (ITP52_19315) comEC 3788150..3790480 (+) 2331 WP_041850028.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  ITP52_RS19320 (ITP52_19320) yqzM 3790520..3790654 (-) 135 WP_003229983.1 YqzM family protein -
  ITP52_RS19325 (ITP52_19325) - 3790695..3790844 (+) 150 WP_120363468.1 hypothetical protein -
  ITP52_RS19330 (ITP52_19330) holA 3790884..3791927 (+) 1044 WP_029317888.1 DNA polymerase III subunit delta -
  ITP52_RS19335 (ITP52_19335) rpsT 3791942..3792208 (-) 267 WP_003229989.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21821.62 Da        Isoelectric Point: 4.7220

>NTDB_id=445059 ITP52_RS19305 WP_041850029.1 3786893..3787510(+) (comEA) [Bacillus subtilis strain SH1]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEVQVNLAEILQDGIVVYIPKKGEETAVQQGGGVSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=445059 ITP52_RS19305 WP_041850029.1 3786893..3787510(+) (comEA) [Bacillus subtilis strain SH1]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCAATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGTGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGATAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGTGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

SLBB domain (SLBB)

(63-117)

Helix-hairpin-helix motif (HHH_3)

(142-203)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.049

100

0.98
  comEA Staphylococcus aureus MW2

36.818

100

0.395
  comEA Staphylococcus aureus N315

35.455

100

0.38