Detailed information NTG0444367

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: HMI21_RS21630
• Genome accession: NZ_CP053358
• Coordinates: 4375592..4376392 (+)
• Length: 266 a.a.
• NCBI ID: WP_004145898.1
• Uniprot ID: -
• Organism: Klebsiella pneumoniae strain EGKP6
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4370592..4381392

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HMI21_RS21595 (HMI21_21915)	gspH	4370748..4371263 (+)	516	WP_004191951.1	type II secretion system minor pseudopilin GspH	-
HMI21_RS21600 (HMI21_21920)	gspI	4371260..4371625 (+)	366	WP_002889025.1	type II secretion system minor pseudopilin GspI	-
HMI21_RS21605 (HMI21_21925)	gspJ	4371622..4372218 (+)	597	WP_023302298.1	type II secretion system minor pseudopilin GspJ	-
HMI21_RS21610 (HMI21_21930)	gspK	4372215..4373201 (+)	987	WP_004177385.1	type II secretion system minor pseudopilin GspK	-
HMI21_RS21615 (HMI21_21935)	gspL	4373188..4374381 (+)	1194	WP_029498035.1	type II secretion system protein GspL	-
HMI21_RS21620 (HMI21_21940)	-	4374374..4374859 (+)	486	WP_004145896.1	type II secretion system protein M	-
HMI21_RS21625 (HMI21_21945)	-	4374849..4375595 (+)	747	WP_004145897.1	type II secretion system protein N	-
HMI21_RS21630 (HMI21_21950)	pilD	4375592..4376392 (+)	801	WP_004145898.1	prepilin peptidase	Machinery gene
HMI21_RS21635 (HMI21_21955)	hrpB	4376440..4378869 (-)	2430	WP_023302300.1	ATP-dependent helicase HrpB	-
HMI21_RS21640 (HMI21_21960)	thpR	4378942..4379478 (+)	537	WP_004177389.1	RNA 2',3'-cyclic phosphodiesterase	-
HMI21_RS21645 (HMI21_21965)	sfsA	4379478..4380194 (+)	717	WP_002888848.1	DNA/RNA nuclease SfsA	-
HMI21_RS21650 (HMI21_21970)	dksA	4380357..4380812 (+)	456	WP_002888845.1	RNA polymerase-binding protein DksA	-

Sequence

Protein

• Length: 266 a.a.
• Molecular weight: 29389.01 Da
• Isoelectric Point: 8.8894

>NTDB_id=444367 HMI21_RS21630 WP_004145898.1 4375592..4376392(+) (pilD) [Klebsiella pneumoniae strain EGKP6]
MTTLAALSLHFPFVWYGFLLLFGLALGSFYNVVIYRLPRMLTQTADDERITLSTPGSSCPQCRQPIAWRDNIPLLSFLWL
GRRARCCQAPIAWSYPLTELATGLLFILAGALLAPGLPLAGGLVLLSFLLILARIDARTQLLPDRLTLPLLWAGLLFNLN
EVYIALPDAVAGAMAGYLALWSVYWLFRLLTGKEALGYGDFKLLAALGAWCGWQVLPQVLLLASASGLVWTLLQRLWTRQ
SLQQPLAFGPWLALAGGGIFLWQQMV

Nucleotide

• Length: 801 bp

>NTDB_id=444367 HMI21_RS21630 WP_004145898.1 4375592..4376392(+) (pilD) [Klebsiella pneumoniae strain EGKP6]
ATGACGACACTAGCCGCGTTATCGCTGCACTTTCCCTTTGTCTGGTATGGCTTTCTACTGCTGTTCGGCCTGGCGCTGGG
CAGCTTTTACAATGTGGTGATCTATCGTCTGCCGCGCATGTTGACCCAGACCGCGGACGACGAGCGGATAACCCTCAGCA
CCCCGGGCTCTTCCTGCCCGCAGTGCCGCCAGCCGATCGCCTGGCGGGACAATATCCCGCTGCTCAGTTTCCTCTGGCTG
GGCCGTCGCGCCCGCTGCTGTCAGGCGCCCATCGCGTGGAGCTATCCGCTGACCGAGCTGGCCACCGGCCTGCTGTTTAT
CCTCGCCGGAGCTTTGCTGGCGCCGGGCCTGCCGCTGGCGGGCGGCCTGGTGCTGCTCTCGTTCCTGCTGATACTGGCGC
GGATTGACGCCCGGACCCAGCTGCTGCCCGATCGCCTGACTCTGCCCCTGCTGTGGGCCGGGCTGCTGTTCAATCTTAAC
GAGGTGTACATTGCCCTGCCGGATGCGGTCGCCGGGGCGATGGCCGGTTATCTGGCGCTGTGGTCGGTCTACTGGCTGTT
CCGCCTGCTGACCGGGAAAGAGGCGCTGGGCTATGGCGATTTTAAGCTGCTGGCGGCGCTGGGCGCCTGGTGCGGCTGGC
AGGTTCTGCCGCAGGTGCTGTTGCTCGCTTCGGCCAGCGGGCTGGTGTGGACCCTGCTGCAACGCCTGTGGACCCGGCAG
TCGCTGCAGCAGCCGCTGGCCTTCGGCCCCTGGCTGGCGCTGGCCGGCGGGGGTATTTTTCTCTGGCAGCAGATGGTCTA
G

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-109)

• Type IV leader peptidase family (Peptidase_A24) (123-231)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	49.434	99.624	0.492
pilD	Vibrio campbellii strain DS40M4	46.182	100	0.477
pilD	Acinetobacter nosocomialis M2	46.008	98.872	0.455
pilD	Acinetobacter baumannii D1279779	45.247	98.872	0.447
pilD	Neisseria gonorrhoeae MS11	43.295	98.12	0.425