NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	ITG66_RS12710	Genome accession	NZ_CP064393
Coordinates	2713381..2714052 (+)	Length	223 a.a.
NCBI ID	WP_003090494.1	Uniprot ID	A0A0H2ZC88
Organism	Pseudomonas aeruginosa strain SRRSH1101
Function	repress competence development (predicted from homology) Competence regulation

Genomic Context

Location: 2708381..2719052

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ITG66_RS12675 (ITG66_12670)	ppyR	2708854..2709111 (+)	258	WP_003090502.1	psl/pyoverdine operon transcriptional regulator PpyR	-
ITG66_RS12680 (ITG66_12675)	-	2709098..2710291 (+)	1194	WP_003122598.1	NnrS family protein	-
ITG66_RS12685 (ITG66_12680)	-	2710292..2710558 (-)	267	WP_003115326.1	DUF5329 domain-containing protein	-
ITG66_RS12690 (ITG66_12685)	-	2710542..2711435 (-)	894	WP_003115327.1	hypothetical protein	-
ITG66_RS12695 (ITG66_12690)	-	2711462..2712544 (-)	1083	WP_003113385.1	hypothetical protein	-
ITG66_RS12700 (ITG66_12695)	-	2712759..2713067 (+)	309	WP_003090497.1	PepSY domain-containing protein	-
ITG66_RS12705 (ITG66_12700)	-	2713067..2713381 (+)	315	WP_003097704.1	PepSY domain-containing protein	-
ITG66_RS12710 (ITG66_12705)	ciaR	2713381..2714052 (+)	672	WP_003090494.1	two-component system response regulator CarR	Regulator
ITG66_RS12715 (ITG66_12710)	carS	2714049..2715386 (+)	1338	WP_191519433.1	two-component system sensor histidine kinase CarS	-
ITG66_RS12720 (ITG66_12715)	-	2715477..2715785 (+)	309	WP_003116713.1	hypothetical protein	-
ITG66_RS12725 (ITG66_12720)	-	2715790..2717934 (-)	2145	WP_031755630.1	methyl-accepting chemotaxis protein	-

Sequence

Protein

Download Length: 223 a.a. Molecular weight: 24729.20 Da Isoelectric Point: 5.8445

                                >NTDB_id=443860 ITG66_RS12710 WP_003090494.1 2713381..2714052(+) (ciaR) [Pseudomonas aeruginosa strain SRRSH1101]

MRLLLVEDHVPLADELMASLTRQGYAVDWLADGRDAAVQGASEPYDLIILDLGLPGRPGLEILQEWRGLGLATPVLILTA

RGSWAERIDGLKAGADDYLTKPFHPEELALRIQALLRRAHGLANQSQLEAAGLRLDEQRQCVCLNGADVDLTAAEFRLLR

YFMLHPGQVLSKGHLAEHLYDGETERDSNVIEVHVNHLRRKLGREVIETRRGQGYRYAGVAAG

Nucleotide

Download Length: 672 bp

                                >NTDB_id=443860 ITG66_RS12710 WP_003090494.1 2713381..2714052(+) (ciaR) [Pseudomonas aeruginosa strain SRRSH1101]

ATGCGGTTGCTGCTGGTTGAGGATCACGTTCCCCTGGCCGATGAACTGATGGCCAGCCTGACCCGCCAGGGCTACGCGGT

GGACTGGCTGGCCGACGGCCGCGACGCGGCGGTGCAGGGCGCCAGCGAGCCCTACGATCTGATCATCCTCGACCTCGGCC

TGCCCGGGCGTCCCGGCCTGGAGATACTCCAGGAGTGGCGTGGGCTGGGACTCGCGACTCCGGTGCTGATCCTCACCGCG

CGCGGTTCCTGGGCCGAACGCATCGATGGGCTCAAGGCCGGTGCCGACGACTACCTGACCAAGCCGTTCCATCCGGAAGA

GCTGGCCTTGCGCATCCAGGCCCTGTTGCGCCGCGCCCATGGGCTGGCCAACCAGAGCCAGCTGGAGGCCGCCGGGCTGC

GCCTGGACGAGCAGCGCCAGTGCGTGTGCCTGAACGGCGCCGACGTCGACCTGACCGCCGCCGAATTCCGCCTGCTGCGC

TATTTCATGCTGCACCCCGGCCAGGTCCTGTCCAAGGGGCACCTGGCCGAGCATCTGTACGACGGCGAGACCGAACGCGA

TTCCAACGTCATCGAGGTCCACGTCAACCACCTGCGGCGCAAGCTCGGCCGCGAGGTGATCGAGACCCGCCGTGGCCAGG

GCTACCGCTACGCCGGGGTGGCCGCCGGATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(147-216)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H2ZC88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus mutans UA159	37.727	98.655	0.372
ciaR	Streptococcus pneumoniae Rx1	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae D39	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae R6	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae TIGR4	37.273	98.655	0.368
micA	Streptococcus pneumoniae Cp1015	35.683	100	0.363
vicR	Streptococcus mutans UA159	35.526	100	0.363

Detailed information NTG0443860