Detailed information NTG0044323

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: FRAAU_RS12455
• Genome accession: NC_017033
• Coordinates: 2686495..2687655 (-)
• Length: 386 a.a.
• NCBI ID: WP_014403878.1
• Uniprot ID: H8L6Q9
• Organism: Frateuria aurantia DSM 6220
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2681495..2692655

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
FRAAU_RS12440 (Fraau_2513)	-	2681553..2682005 (+)	453	WP_014403875.1	DUF4442 domain-containing protein	-
FRAAU_RS12445 (Fraau_2514)	-	2682166..2684013 (+)	1848	WP_014403876.1	M14 family metallopeptidase	-
FRAAU_RS12450 (Fraau_2515)	-	2684118..2686271 (+)	2154	WP_014403877.1	M13-type metalloendopeptidase	-
FRAAU_RS12455 (Fraau_2516)	pilU	2686495..2687655 (-)	1161	WP_014403878.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
FRAAU_RS12460 (Fraau_2517)	pilT	2687690..2688727 (-)	1038	WP_014403879.1	type IV pilus twitching motility protein PilT	Machinery gene
FRAAU_RS12465 (Fraau_2518)	proC	2688898..2689725 (+)	828	WP_014403880.1	pyrroline-5-carboxylate reductase	-
FRAAU_RS12470 (Fraau_2519)	-	2689722..2690294 (+)	573	WP_014403881.1	YggT family protein	-
FRAAU_RS12475 (Fraau_2520)	rlmB	2690509..2691255 (-)	747	WP_014403882.1	23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB	-

Sequence

Protein

• Length: 386 a.a.
• Molecular weight: 42412.51 Da
• Isoelectric Point: 5.8490

>NTDB_id=44323 FRAAU_RS12455 WP_014403878.1 2686495..2687655(-) (pilU) [Frateuria aurantia DSM 6220]
MSEMIDGWLTAMVERGASDLFVTAGAPPALKINGEVKSIDAEPLSDVQTRDIVLGMMSPAQIQEFEQTRECQFALSRRDV
GRFRVSAFFQRSSMGAVLRRIETRIPTVDELSLPAVIPSLAMMKRGLVIVAGGTGTGKSTTLAAMLGYRNSHSTGHIITV
EDPIEFVHRHQGCIVSQRELGIDTESWGSALKNTLRQAPDVILIGEVRTREGMEYAINFAETGHLCLCTLHANNANQAIE
RIISFFPADQHAHLLLDLSLNLKGIVAQQLVSRPDGQSRQAILEVLLGTPLVQDLIRTGDIVRIKEVMRNSNEAGMCTFD
QSLIHAYQQGKITYEDALRYADSANEVRLQIKLATPGSANALLAETSKISLQQMDEGDSLFDTRRP

Nucleotide

• Length: 1161 bp

>NTDB_id=44323 FRAAU_RS12455 WP_014403878.1 2686495..2687655(-) (pilU) [Frateuria aurantia DSM 6220]
ATGAGCGAGATGATCGATGGCTGGCTGACCGCCATGGTCGAGAGGGGTGCTTCGGACCTGTTCGTCACTGCCGGGGCGCC
GCCGGCCTTGAAGATCAATGGCGAGGTCAAGTCCATCGATGCGGAGCCGCTCAGTGATGTCCAGACCCGCGACATCGTGC
TGGGCATGATGAGTCCGGCCCAGATCCAGGAATTCGAGCAGACCAGGGAATGCCAGTTCGCCCTCAGCCGTCGTGACGTG
GGCCGTTTCCGCGTGTCGGCGTTCTTTCAGCGCAGCAGCATGGGCGCGGTGCTGCGTCGGATCGAGACGCGGATTCCGAC
GGTGGACGAACTCAGTCTGCCGGCGGTGATTCCTTCGCTGGCCATGATGAAGCGCGGTCTGGTGATCGTGGCCGGCGGGA
CCGGCACCGGCAAGTCGACCACGCTGGCAGCGATGCTGGGCTATCGCAATAGCCATTCGACCGGGCATATCATCACCGTC
GAGGATCCGATCGAATTCGTGCACCGGCATCAAGGCTGCATCGTCAGTCAGCGCGAGCTCGGCATCGATACCGAGAGCTG
GGGATCGGCACTGAAGAATACCTTGCGACAGGCGCCCGACGTGATCCTGATCGGGGAGGTCCGTACGCGCGAAGGCATGG
AATATGCCATCAACTTCGCCGAGACCGGGCATCTATGCCTGTGCACCCTGCATGCCAACAACGCCAACCAGGCGATCGAG
CGCATCATCAGCTTCTTCCCGGCCGATCAGCATGCCCATCTGCTGCTGGATCTGTCGCTGAATCTGAAAGGGATCGTGGC
CCAGCAACTGGTAAGCCGTCCAGACGGCCAGTCCAGGCAGGCGATACTCGAAGTACTGTTGGGGACGCCGCTGGTGCAGG
ACCTGATCCGTACCGGTGACATCGTCAGAATCAAGGAGGTGATGCGCAACTCCAACGAGGCCGGCATGTGCACCTTCGAC
CAGAGCCTGATCCATGCCTATCAGCAGGGCAAGATCACCTACGAAGACGCCTTGCGCTATGCCGACAGTGCCAACGAGGT
GCGGCTGCAGATCAAGCTGGCGACCCCGGGCAGTGCCAACGCACTGTTGGCCGAGACTTCCAAGATCAGTCTGCAGCAGA
TGGACGAGGGCGACAGCTTGTTCGATACCCGTCGGCCCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (119-272)

3D structure

• AlphaFold DB: H8L6Q9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	59.511	95.337	0.567
pilU	Acinetobacter baylyi ADP1	55.858	95.078	0.531
pilU	Vibrio cholerae strain A1552	57.759	90.155	0.521
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.544	86.269	0.376