Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   DBV21_RS01985 Genome accession   NZ_CP053029
Coordinates   433820..434884 (+) Length   354 a.a.
NCBI ID   WP_003414236.1    Uniprot ID   A0A0P9RSF6
Organism   Pseudomonas congelans strain TLP2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 428820..439884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DBV21_RS01975 (DBV21_02125) - 429683..430951 (+) 1269 WP_003365988.1 malic enzyme-like NAD(P)-binding protein -
  DBV21_RS01980 (DBV21_02135) - 431169..433610 (-) 2442 WP_420851502.1 penicillin-binding protein 1A -
  DBV21_RS01985 (DBV21_02140) pilM 433820..434884 (+) 1065 WP_003414236.1 pilus assembly protein PilM Machinery gene
  DBV21_RS01990 (DBV21_02145) - 434884..435459 (+) 576 WP_214348702.1 PilN domain-containing protein -
  DBV21_RS01995 (DBV21_02150) pilO 435456..436079 (+) 624 WP_010438765.1 type 4a pilus biogenesis protein PilO -
  DBV21_RS02000 (DBV21_02155) pilP 436076..436603 (+) 528 WP_003350151.1 type 4a pilus biogenesis lipoprotein PilP -
  DBV21_RS02005 (DBV21_02160) pilQ 436651..438756 (+) 2106 WP_010438770.1 type IV pilus secretin PilQ Machinery gene
  DBV21_RS02010 (DBV21_02165) aroK 438761..439279 (+) 519 WP_214348705.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38120.65 Da        Isoelectric Point: 4.7049

>NTDB_id=442253 DBV21_RS01985 WP_003414236.1 433820..434884(+) (pilM) [Pseudomonas congelans strain TLP2]
MFELFSKKANTLLGIDISSTSVKLLELSRSGTRYKVESYAVEPLPANAVVEKNIAELEGVGQALSRVLVKAKTSVKIVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKLEADQYIPYPLEEVAIDFEVQGYSVRNPERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLAAQLGNGHDELTVAVVDIGATMTTLSVLHHGRIIYTREQLFGGRQLTDEIQRRYGLSM
EEAGLAKKQGGLPDDYVSEVLDPFKEALVQQVSRSLQFFFAAGQYNSVDHIMLAGGTASISGLEHLIQRRIGTPTMVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=442253 DBV21_RS01985 WP_003414236.1 433820..434884(+) (pilM) [Pseudomonas congelans strain TLP2]
GTGTTCGAACTCTTCAGTAAGAAGGCCAACACCCTTCTAGGGATCGATATTAGCTCCACCTCGGTAAAACTCCTGGAATT
GAGTCGTTCCGGCACCCGTTACAAGGTCGAGTCTTACGCAGTTGAGCCATTGCCGGCCAACGCTGTCGTCGAAAAGAATA
TTGCCGAACTCGAAGGGGTCGGTCAGGCGTTGTCACGCGTACTCGTCAAGGCCAAGACCAGCGTCAAGATCGTGGCGGTT
GCCGTGGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGACATGGAAAACCAGCT
CAAGCTTGAAGCTGACCAGTACATCCCCTATCCGCTGGAAGAAGTTGCCATCGATTTCGAAGTGCAGGGCTACTCGGTGC
GCAATCCCGAGCGCGTCGAGGTGTTGCTGGCGGCCTGCCGCAAAGAGAACGTCGAGGTGCGCGAAGCCGCGCTGGCGCTG
GCCGGACTGACTGCGCGCGTCGTCGACGTCGAGGCCTACGCCCTTGAGCGTTCGTTCGGGCTGCTGGCCGCGCAACTGGG
CAACGGTCATGACGAGCTGACGGTGGCCGTGGTCGATATCGGCGCCACCATGACCACCCTCAGTGTGCTGCATCATGGCC
GGATCATTTACACCCGCGAGCAGCTGTTTGGCGGGCGCCAGCTGACTGACGAAATCCAGCGCCGCTACGGGCTTTCCATG
GAGGAAGCCGGGCTTGCCAAGAAGCAGGGCGGCTTGCCTGACGACTACGTCAGCGAGGTGCTCGATCCCTTCAAGGAAGC
GCTGGTGCAGCAGGTTTCCCGATCGCTGCAGTTCTTCTTTGCAGCCGGTCAATACAACTCCGTGGATCACATCATGCTGG
CAGGCGGCACGGCTTCGATTTCAGGGCTGGAGCATCTGATCCAGCGCCGCATCGGCACTCCGACCATGGTCGCCAACCCG
TTTGCCGATATGGCCCTGAGCTCCAAGGTCAACGCCGGCGCCCTGGCCAGTGATGCGCCGGCGCTGATGATCGCCTGTGG
CCTGGCGTTGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0P9RSF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

53.955

100

0.54
  pilM Legionella pneumophila strain ERS1305867

46.479

100

0.466