Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   IRJ26_RS14835 Genome accession   NZ_CP064094
Coordinates   2849956..2850960 (+) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain N1108-5at     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2844956..2855960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ26_RS14805 - 2845185..2845307 (+) 123 WP_003229707.1 hypothetical protein -
  IRJ26_RS14810 yrzF 2845353..2846000 (-) 648 WP_003246159.1 phosphotransferase -
  IRJ26_RS14815 iolG 2846238..2847263 (+) 1026 WP_004398990.1 inositol 2-dehydrogenase -
  IRJ26_RS14820 csbX 2847334..2848641 (+) 1308 WP_003246197.1 MFS transporter -
  IRJ26_RS14825 bofC 2848689..2849201 (+) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  IRJ26_RS14830 ruvA 2849340..2849945 (+) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  IRJ26_RS14835 ruvB 2849956..2850960 (+) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  IRJ26_RS14840 yrzS 2850953..2851153 (+) 201 WP_003222669.1 DUF2905 domain-containing protein -
  IRJ26_RS14845 queA 2851183..2852211 (+) 1029 WP_003229723.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  IRJ26_RS14850 tgt 2852238..2853383 (+) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  IRJ26_RS14855 yajC 2853420..2853686 (+) 267 WP_004398708.1 preprotein translocase subunit YajC -
  IRJ26_RS14860 - 2853743..2854132 (-) 390 WP_004398667.1 TIGR04086 family membrane protein -
  IRJ26_RS14865 yrbG 2854330..2854986 (+) 657 WP_004398801.1 DUF421 domain-containing protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=441926 IRJ26_RS14835 WP_003229718.1 2849956..2850960(+) (ruvB) [Bacillus subtilis strain N1108-5at]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=441926 IRJ26_RS14835 WP_003229718.1 2849956..2850960(+) (ruvB) [Bacillus subtilis strain N1108-5at]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGCCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTAATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518