Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HKJ31_RS10255 Genome accession   NZ_CP052855
Coordinates   2264336..2266069 (+) Length   577 a.a.
NCBI ID   WP_004084598.1    Uniprot ID   A0A9Q4QTQ0
Organism   Xylella fastidiosa subsp. multiplex strain Fillmore     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2259336..2271069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HKJ31_RS10240 (HKJ31_10440) pilC 2259665..2260867 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  HKJ31_RS10245 (HKJ31_10445) - 2261308..2261754 (+) 447 WP_154128445.1 pilin -
  HKJ31_RS10250 (HKJ31_10455) - 2263062..2263496 (+) 435 WP_004084597.1 pilin -
  HKJ31_RS10255 (HKJ31_10460) pilB 2264336..2266069 (+) 1734 WP_004084598.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HKJ31_RS10260 (HKJ31_10465) pilR 2266163..2267575 (-) 1413 WP_020851962.1 sigma-54 dependent transcriptional regulator Regulator
  HKJ31_RS10265 (HKJ31_10470) - 2267762..2269372 (-) 1611 WP_004084601.1 ATP-binding protein -
  HKJ31_RS10270 (HKJ31_10475) sucC 2269627..2270790 (+) 1164 WP_004084603.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63030.16 Da        Isoelectric Point: 6.0946

>NTDB_id=441751 HKJ31_RS10255 WP_004084598.1 2264336..2266069(+) (pilB) [Xylella fastidiosa subsp. multiplex strain Fillmore]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSAAKLAAANALEFGMPLLDVSAFDHN
HNAMNLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALE
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFINKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKAVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPK
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=441751 HKJ31_RS10255 WP_004084598.1 2264336..2266069(+) (pilB) [Xylella fastidiosa subsp. multiplex strain Fillmore]
ATGAATACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TGAGTGCAGCAAAATTGGCCGCGGCCAATGCACTTGAGTTCGGGATGCCGCTGCTGGATGTGTCCGCATTCGACCACAAT
CACAATGCAATGAACTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGACGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAG
GAAGAGGATAACGGCATTGAAGGGCTAGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACCGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCATCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATTC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGCTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATCTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTACTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTACTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTGTCACTTTA
CACTGCGTTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGTG
TCAATCAGGTACAACAAAACAACAAGCGCGGTATGACGTTTGCCGCAGCGTTACGTTCGTTTCTGCGCCAAGACCCGGAT
GTGATCATGGTCGGCGAAATCCGGGATCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACACTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAACAAGTGCAAGCGCCCGGTTCAATTGCCGAAA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCACAGCGCATCGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.319

98.267

0.563
  pilB Acinetobacter baumannii D1279779

56.79

98.267

0.558
  pilB Legionella pneumophila strain ERS1305867

51.408

98.44

0.506
  pilF Neisseria gonorrhoeae MS11

50.615

98.614

0.499
  pilB Vibrio cholerae strain A1552

50

94.281

0.471
  pilB Vibrio campbellii strain DS40M4

46.914

98.267

0.461
  pilB Vibrio parahaemolyticus RIMD 2210633

46.749

98.614

0.461
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.806

92.894

0.36