Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IRJ24_RS13655 Genome accession   NZ_CP064093
Coordinates   2617046..2617456 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain N1282-4at     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2612046..2622456
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IRJ24_RS13620 arsR 2612209..2612526 (+) 318 WP_004399122.1 arsenical resistance operon transcriptional regulator ArsR -
  IRJ24_RS13625 yqcK 2612587..2613027 (+) 441 WP_003229954.1 ArsI/CadI family heavy metal resistance metalloenzyme -
  IRJ24_RS13630 acr3 2613050..2614090 (+) 1041 WP_004398718.1 arsenite efflux transporter Acr3 -
  IRJ24_RS13635 arsC 2614102..2614521 (+) 420 WP_004398596.1 thioredoxin-dependent arsenate reductase -
  IRJ24_RS13640 - 2614876..2615054 (+) 179 Protein_2613 hypothetical protein -
  IRJ24_RS13645 spoIVCA 2615012..2616472 (+) 1461 WP_223257626.1 site-specific DNA recombinase SpoIVCA -
  IRJ24_RS13650 - 2616503..2616850 (-) 348 Protein_2615 sigma-70 family RNA polymerase sigma factor -
  IRJ24_RS13655 nucA/comI 2617046..2617456 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  IRJ24_RS13660 yqeB 2617489..2618211 (-) 723 WP_010886572.1 hypothetical protein -
  IRJ24_RS13665 gnd 2618463..2619356 (+) 894 WP_003229961.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  IRJ24_RS13670 yqeD 2619375..2620001 (-) 627 WP_003229962.1 TVP38/TMEM64 family protein -
  IRJ24_RS13675 cwlH 2620188..2620940 (+) 753 WP_003229963.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  IRJ24_RS13680 yqeF 2621192..2621923 (+) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  IRJ24_RS13685 - 2622229..2622369 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=441750 IRJ24_RS13655 WP_009967785.1 2617046..2617456(+) (nucA/comI) [Bacillus subtilis strain N1282-4at]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=441750 IRJ24_RS13655 WP_009967785.1 2617046..2617456(+) (nucA/comI) [Bacillus subtilis strain N1282-4at]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGTACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529