Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   HHK01_RS13375 Genome accession   NZ_CP052135
Coordinates   2698780..2699928 (-) Length   382 a.a.
NCBI ID   WP_228027577.1    Uniprot ID   -
Organism   Bacillus fonticola strain CS13     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2693780..2704928
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHK01_RS13345 - 2695059..2695541 (-) 483 WP_170008236.1 flagellar protein FlgN -
  HHK01_RS13350 flgM 2695557..2695832 (-) 276 WP_170008237.1 flagellar biosynthesis anti-sigma factor FlgM -
  HHK01_RS13355 - 2696019..2696618 (+) 600 WP_170008238.1 hypothetical protein -
  HHK01_RS13360 - 2697036..2697407 (+) 372 WP_170008239.1 VOC family protein -
  HHK01_RS13365 - 2697454..2697882 (-) 429 WP_170008240.1 TIGR03826 family flagellar region protein -
  HHK01_RS13370 - 2698004..2698477 (-) 474 WP_170008241.1 ComF family protein -
  HHK01_RS13375 comFA 2698780..2699928 (-) 1149 WP_228027577.1 DEAD/DEAH box helicase Machinery gene
  HHK01_RS13380 - 2700470..2701330 (-) 861 WP_170008802.1 DegV family protein -
  HHK01_RS13385 degU 2701618..2702307 (-) 690 WP_170008243.1 response regulator transcription factor Regulator
  HHK01_RS13390 degS 2702344..2703483 (-) 1140 WP_170008244.1 sensor histidine kinase Regulator
  HHK01_RS13395 - 2703869..2704513 (+) 645 WP_170008245.1 YigZ family protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 42867.58 Da        Isoelectric Point: 10.5483

>NTDB_id=441456 HHK01_RS13375 WP_228027577.1 2698780..2699928(-) (comFA) [Bacillus fonticola strain CS13]
MGRVAVCTSLYEWTGPPSRWKDSSALHWGGELSPAQRRASDRVIEAVSNDQSLLVWAVCGSGKTEVLFRGIERALQQGKR
VCIAAPRTDVILELAPRLQEVFPYIPVATLYGGAPNRFATSSLTLCTTHQLLRFSHAFDVIVVDEVDAFPYSVDATLQFA
VRKALKTNTSPIVYLTATPPRYFQKACRQGVQPYVRIPARYHRNPLPEPTFVRCFSWRKAVEKGRLPTKALQWMNARLEQ
GRRVLLFFPNIHLMQKVEKGLLKHLPEATSVHAEDPARKEKVQALRVGDRRVLLTTTILERGVTIPFVDVAVIGGEHRQF
TEAALVQIAGRAGRKKDDTMGQVCFFHNGVSLSMLRARHHIRSMNREALANGDLDFRGGPRP

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=441456 HHK01_RS13375 WP_228027577.1 2698780..2699928(-) (comFA) [Bacillus fonticola strain CS13]
ATGGGGCGTGTCGCAGTATGTACGAGCCTTTATGAATGGACAGGTCCACCCAGTCGGTGGAAAGATTCTTCAGCTTTGCA
CTGGGGGGGTGAGTTGTCACCTGCACAAAGGCGTGCCAGTGACCGGGTGATAGAGGCCGTTTCAAACGATCAGTCCCTAT
TGGTTTGGGCGGTTTGCGGATCTGGAAAAACAGAAGTGCTATTTCGCGGGATTGAGCGTGCATTACAGCAAGGCAAAAGG
GTGTGCATCGCCGCACCGCGAACGGATGTGATTTTAGAGCTTGCTCCTCGGTTGCAGGAAGTATTTCCGTATATACCCGT
AGCCACTTTATACGGAGGTGCGCCAAATCGTTTTGCAACAAGTTCCCTCACCTTATGTACTACCCATCAATTGCTTCGTT
TCTCCCACGCGTTTGATGTAATCGTTGTCGATGAAGTCGACGCGTTTCCGTACTCTGTTGACGCCACGCTGCAATTTGCC
GTTCGAAAGGCGTTAAAAACCAACACATCCCCGATTGTCTATTTGACCGCTACCCCACCGCGTTATTTCCAAAAAGCATG
CCGCCAAGGTGTCCAGCCCTACGTTCGCATTCCTGCACGATATCACCGTAACCCGCTTCCCGAACCGACATTTGTCCGTT
GCTTTTCATGGCGGAAGGCGGTTGAAAAAGGGCGCTTGCCTACAAAAGCACTTCAGTGGATGAACGCACGGCTTGAACAA
GGTCGTCGTGTACTACTCTTCTTTCCAAACATTCATCTCATGCAAAAAGTTGAAAAAGGCCTCTTGAAACACCTTCCAGA
AGCAACAAGCGTTCATGCCGAAGATCCCGCGCGGAAGGAGAAGGTACAAGCGTTACGCGTAGGAGATCGCCGGGTGTTAC
TGACGACAACCATTTTAGAGCGTGGCGTCACGATTCCGTTTGTCGATGTGGCGGTTATTGGCGGTGAGCATCGCCAATTT
ACAGAAGCTGCACTAGTACAAATTGCTGGGCGAGCCGGACGAAAAAAGGACGATACGATGGGACAGGTTTGTTTCTTTCA
TAATGGAGTTTCGCTAAGTATGCTGCGGGCACGACATCACATTCGAAGTATGAATCGTGAAGCATTAGCTAATGGAGATC
TTGACTTTAGAGGGGGACCGCGCCCATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(31-181)

Helicase conserved C-terminal domain (Helicase_C)

(238-335)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

51.762

96.597

0.5
  comFA/cflA Streptococcus mitis SK321

38.978

97.382

0.38
  comFA Latilactobacillus sakei subsp. sakei 23K

41.983

89.791

0.377
  comFA/cflA Streptococcus pneumoniae D39

38.172

97.382

0.372
  comFA/cflA Streptococcus pneumoniae Rx1

38.172

97.382

0.372
  comFA/cflA Streptococcus pneumoniae R6

38.172

97.382

0.372
  comFA/cflA Streptococcus mitis NCTC 12261

38.108

96.859

0.369
  comFA/cflA Streptococcus pneumoniae TIGR4

37.903

97.382

0.369
  comFA Lactococcus lactis subsp. cremoris KW2

37.94

96.597

0.366