Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HG264_RS11290 Genome accession   NZ_CP051625
Coordinates   2448762..2449826 (-) Length   354 a.a.
NCBI ID   WP_169407745.1    Uniprot ID   A0A7Z2ZE75
Organism   Pseudomonas sp. gcc21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2443762..2454826
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HG264_RS11265 (HG264_11260) aroK 2444305..2444811 (-) 507 WP_169407742.1 shikimate kinase AroK -
  HG264_RS11270 (HG264_11265) pilQ 2444811..2446979 (-) 2169 WP_169409106.1 type IV pilus secretin PilQ Machinery gene
  HG264_RS11275 (HG264_11270) - 2447037..2447561 (-) 525 WP_169409107.1 pilus assembly protein PilP -
  HG264_RS11280 (HG264_11275) - 2447567..2448187 (-) 621 WP_169407743.1 type 4a pilus biogenesis protein PilO -
  HG264_RS11285 (HG264_11280) - 2448184..2448762 (-) 579 WP_169407744.1 PilN domain-containing protein -
  HG264_RS11290 (HG264_11285) pilM 2448762..2449826 (-) 1065 WP_169407745.1 pilus assembly protein PilM Machinery gene
  HG264_RS11295 (HG264_11290) - 2450012..2452411 (+) 2400 WP_169407746.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38358.79 Da        Isoelectric Point: 4.3493

>NTDB_id=439943 HG264_RS11290 WP_169407745.1 2448762..2449826(-) (pilM) [Pseudomonas sp. gcc21]
MLGLIKKKSNTLLGIDISSTTVKLLELSRSGNRYKVEAYAVEPLPPSAVVEKNIVELEGVGQALEKVLARSRTSVKQAAV
AVAGSAVITKIIEMDAGFDDDELEEQIKLEADQYIPYPLDEVAIDFEVQGISPRNPERVEVLLAACRRENVDIREAALAL
AGIDVKVVEVEAYAMERACSLVTDQLDSGQHDLTIAVVDIGATMMTLSVLSQGRTIYTREQLFGGKQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYETEVLGPFKDAVVQQVSRSLQFFFAGNQYNDVDYILLAGGTSSIPGLDQLIQQKLGTTTLVANP
FANMTLSNKVNAVALSNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=439943 HG264_RS11290 WP_169407745.1 2448762..2449826(-) (pilM) [Pseudomonas sp. gcc21]
GTGCTAGGGCTCATAAAAAAGAAATCGAATACGCTTCTGGGTATCGATATCAGCTCCACGACTGTGAAGCTGCTAGAACT
CAGTCGCTCCGGGAACCGCTATAAGGTCGAGGCCTACGCGGTCGAACCTCTCCCACCGAGCGCGGTGGTCGAGAAAAACA
TTGTCGAGCTCGAAGGCGTCGGCCAGGCCCTGGAAAAAGTCCTGGCGCGATCCCGTACCTCGGTGAAACAGGCAGCCGTC
GCCGTAGCGGGCTCAGCGGTGATCACCAAGATCATCGAAATGGATGCCGGTTTCGATGATGATGAGCTTGAAGAACAGAT
CAAGCTTGAGGCTGACCAATATATCCCTTATCCCCTGGACGAAGTTGCGATCGATTTCGAAGTCCAGGGAATTTCCCCCC
GAAATCCTGAGCGAGTCGAAGTATTGCTGGCGGCCTGCCGCCGGGAAAATGTCGACATTCGTGAAGCGGCTCTCGCATTG
GCTGGCATCGACGTCAAAGTCGTTGAAGTTGAAGCCTACGCCATGGAGCGCGCCTGTAGCCTGGTTACCGACCAGTTGGA
CAGCGGCCAGCATGATCTGACCATTGCCGTCGTGGATATCGGCGCGACGATGATGACGCTCAGTGTGCTTAGCCAGGGCC
GGACTATCTACACCCGCGAGCAGTTATTCGGGGGCAAGCAGTTGACTGAAGAGATTCAGCGTCGCTACGGTCTTTCGCAG
GAAGAGGCAGGGTTGGCCAAGAAGCAGGGCGGTCTACCGGACGATTATGAAACAGAGGTGCTGGGGCCTTTCAAGGATGC
TGTGGTCCAGCAAGTGTCGCGCTCGCTGCAGTTTTTCTTCGCGGGCAATCAGTACAACGACGTCGACTACATCCTCCTTG
CAGGTGGCACATCGTCGATTCCCGGGCTCGATCAATTGATCCAGCAGAAGCTCGGGACCACGACGCTGGTGGCCAACCCC
TTTGCAAACATGACCCTTTCCAACAAGGTAAATGCGGTTGCGCTCAGTAATGACGCGCCGGCATTGATGATTGCCTGCGG
TCTGGCAATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2ZE75

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

46.045

100

0.46