Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   HG264_RS11270 Genome accession   NZ_CP051625
Coordinates   2444811..2446979 (-) Length   722 a.a.
NCBI ID   WP_169409106.1    Uniprot ID   A0A7Z2V6U0
Organism   Pseudomonas sp. gcc21     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2439811..2451979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HG264_RS11255 (HG264_11250) - 2441595..2443073 (-) 1479 WP_169407740.1 AAA family ATPase -
  HG264_RS11260 (HG264_11255) aroB 2443070..2444161 (-) 1092 WP_169407741.1 3-dehydroquinate synthase -
  HG264_RS11265 (HG264_11260) aroK 2444305..2444811 (-) 507 WP_169407742.1 shikimate kinase AroK -
  HG264_RS11270 (HG264_11265) pilQ 2444811..2446979 (-) 2169 WP_169409106.1 type IV pilus secretin PilQ Machinery gene
  HG264_RS11275 (HG264_11270) - 2447037..2447561 (-) 525 WP_169409107.1 pilus assembly protein PilP -
  HG264_RS11280 (HG264_11275) - 2447567..2448187 (-) 621 WP_169407743.1 type 4a pilus biogenesis protein PilO -
  HG264_RS11285 (HG264_11280) - 2448184..2448762 (-) 579 WP_169407744.1 PilN domain-containing protein -
  HG264_RS11290 (HG264_11285) pilM 2448762..2449826 (-) 1065 WP_169407745.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 722 a.a.        Molecular weight: 77732.02 Da        Isoelectric Point: 4.6168

>NTDB_id=439942 HG264_RS11270 WP_169409106.1 2444811..2446979(-) (pilQ) [Pseudomonas sp. gcc21]
MPLTRKLSLAAALALLNPLAIAADLQTLDVASLPGERVELKLSFDEPVAAPKGYTIEQPARIALDLPGVKNKLGERSRDL
GLGNARSVTVVEAGDRTRLIINLTDLAPYATRVEGNNLFVMVGADQAPGYNAPAKAAAPSASTTASWRAADEPAVAAKSP
AAASGKAIRGLDFQRGEQGEGNVIVDLSDPSVRVDVQEQGGRVRLTFPGTRLPDELRVRLDVKDFATPVNFVNASTSGND
TTINIEPNGFYEHLVYQTDNRLIVSFKPLSKTEVEQRRAADFAYTGEKLSLNFQDIEVRSVLQLIADFTDMNLVASDTVS
GSITLRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQRQIAELAPLRRELIQVNYANAADIANL
FASVTAGEGEQRGSITVDDRTNSIIALETQDKLDELRRIVTQLDIPVRQVMIEARIVEANVGFDKALGVRWGGNLRMGNR
FDLFGKDGQTGVVEDPNARGQLGDEDYIPSRNTGGFIGIPERPFNTPFVDMGVLGATSGVGIGFITNNAVLDLQLSAMES
TGNGEIISQPKVVTSDKETAKILKGSEIPYQEASSSGATTTAFAEAVLSLEVTPQITPDNQVIMEVIVTKDEPDFTNEVN
GVPTIRKNEVNAKILVADGETIVIGGVFSSETSKGQQKVPFLGDLPILGRAFRRDITSESKTELLVFLTPRIINSGAISL
AK

Nucleotide


Download         Length: 2169 bp        

>NTDB_id=439942 HG264_RS11270 WP_169409106.1 2444811..2446979(-) (pilQ) [Pseudomonas sp. gcc21]
ATGCCTCTAACCAGGAAGTTATCGCTTGCAGCAGCCCTGGCGCTGTTGAACCCGTTGGCCATCGCGGCCGACCTTCAGAC
ACTGGACGTCGCCTCGCTGCCGGGTGAACGCGTCGAACTCAAACTCAGTTTTGACGAACCCGTTGCCGCACCGAAAGGCT
ATACCATTGAGCAGCCTGCCCGTATCGCTCTGGATTTGCCTGGCGTGAAGAACAAGCTGGGCGAGCGCAGCCGCGATCTC
GGCCTCGGAAATGCGCGTAGCGTGACCGTGGTTGAAGCCGGTGACCGGACTCGCCTGATCATCAATCTTACCGATCTTGC
GCCTTACGCCACACGGGTCGAAGGCAACAATCTGTTTGTCATGGTCGGCGCCGATCAGGCGCCGGGCTATAACGCTCCGG
CGAAAGCTGCGGCGCCCAGCGCCTCGACAACCGCCAGTTGGCGTGCTGCCGACGAACCGGCCGTGGCAGCAAAATCACCA
GCGGCCGCTTCGGGCAAAGCCATTCGTGGGCTGGATTTCCAGCGCGGTGAACAGGGCGAGGGTAACGTTATCGTCGACTT
GTCCGATCCGTCCGTACGAGTCGACGTGCAGGAACAGGGCGGCCGTGTACGGTTGACCTTCCCTGGCACGCGTCTGCCTG
ATGAGCTGCGCGTACGGTTGGACGTCAAGGATTTCGCAACGCCGGTCAATTTCGTCAACGCCAGCACTAGCGGTAACGAT
ACAACCATCAATATCGAGCCGAACGGATTCTACGAGCATCTGGTATACCAGACCGATAACCGTCTGATCGTCAGCTTCAA
ACCATTGAGCAAGACCGAGGTTGAACAGCGCCGTGCTGCAGATTTCGCTTACACAGGTGAAAAGCTGTCGCTTAATTTCC
AGGACATCGAAGTGCGTTCGGTGCTGCAGTTGATTGCAGATTTCACTGACATGAACCTGGTCGCCAGTGACACCGTCTCA
GGCAGCATCACCTTGCGATTGCAGAATGTGCCTTGGGACCAGGCGCTTGATCTGGTGCTGAAAACCAAGGGGCTGGACAA
GCGTAAAGTAGGTAATGTCCTACTGGTTGCGCCCGCCGACGAGATCGCTGCACGCGAACGCCAGGAGCTGGAATCCCAGC
GGCAGATCGCCGAGCTGGCTCCGCTGCGTCGCGAGCTGATCCAGGTTAACTACGCCAACGCAGCAGACATTGCTAACCTG
TTCGCGTCGGTAACCGCAGGCGAGGGCGAACAGCGCGGCTCGATTACAGTCGATGACCGCACCAATAGCATCATTGCTCT
GGAAACCCAGGACAAGCTGGATGAGCTACGCCGCATCGTTACCCAGCTGGATATTCCGGTTCGCCAGGTCATGATCGAGG
CGCGCATCGTTGAAGCCAACGTGGGTTTCGACAAGGCGCTTGGCGTGCGCTGGGGTGGCAATCTGCGGATGGGTAATCGT
TTCGATCTGTTCGGCAAGGACGGCCAGACCGGTGTGGTCGAAGACCCTAACGCGCGTGGCCAGCTGGGTGATGAGGATTA
TATTCCTTCACGCAACACCGGTGGCTTCATCGGCATCCCTGAGCGTCCTTTCAATACACCGTTCGTAGATATGGGTGTAC
TGGGTGCGACCTCAGGTGTCGGCATCGGGTTTATCACCAACAACGCCGTGCTTGATCTGCAACTGTCGGCAATGGAAAGC
ACCGGTAACGGGGAGATCATTTCTCAGCCCAAGGTCGTGACGTCCGATAAGGAAACCGCGAAAATCCTCAAGGGTTCGGA
GATTCCATACCAGGAAGCCAGCTCCAGCGGTGCAACCACAACCGCCTTTGCCGAAGCCGTACTGTCGCTGGAAGTGACTC
CGCAGATCACGCCGGATAACCAGGTGATCATGGAAGTGATTGTGACCAAGGACGAGCCGGACTTTACCAACGAAGTGAAC
GGGGTGCCGACTATTCGCAAGAATGAAGTCAATGCGAAAATTCTGGTAGCCGATGGAGAAACCATCGTTATCGGTGGGGT
GTTCTCCAGTGAAACTTCAAAAGGTCAGCAGAAGGTTCCGTTCCTGGGCGACCTGCCAATCTTGGGCAGGGCGTTCCGTC
GTGATATAACGTCGGAAAGCAAGACCGAGCTGCTGGTGTTCCTCACGCCACGCATCATTAATAGTGGAGCTATTTCCCTT
GCCAAGTAA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-120)

Secretin and TonB N terminus short domain (STN)

(312-358)

Bacterial type II/III secretion system short domain (Secretin_N)

(386-449)

Bacterial type II and III secretion system protein (Secretin)

(557-713)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2V6U0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

69.051

100

0.695
  pilQ Acinetobacter baumannii D1279779

38.564

100

0.402
  pilQ Acinetobacter baumannii strain A118

38.564

100

0.402
  comQ Acinetobacter baylyi ADP1

40

99.03

0.396