Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   HHM65_RS00785 Genome accession   NZ_CP051624
Coordinates   127946..128977 (+) Length   343 a.a.
NCBI ID   WP_015057201.1    Uniprot ID   -
Organism   Streptococcus dysgalactiae subsp. equisimilis strain TPCH-A74     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 122946..133977
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HHM65_RS00775 (HHM65_00780) - 126623..126988 (+) 366 WP_003055257.1 DUF1033 family protein -
  HHM65_RS00780 (HHM65_00785) comGA/cglA 127069..128010 (+) 942 WP_170078124.1 competence type IV pilus ATPase ComGA Machinery gene
  HHM65_RS00785 (HHM65_00790) comYB 127946..128977 (+) 1032 WP_015057201.1 competence type IV pilus assembly protein ComGB Machinery gene
  HHM65_RS00790 (HHM65_00795) comYC 128979..129305 (+) 327 WP_003055259.1 competence type IV pilus major pilin ComGC Machinery gene
  HHM65_RS00795 (HHM65_00800) comGD 129280..129708 (+) 429 WP_041781473.1 competence type IV pilus minor pilin ComGD -
  HHM65_RS00800 (HHM65_00805) comGE 129716..129961 (+) 246 WP_003058569.1 competence type IV pilus minor pilin ComGE -
  HHM65_RS00805 (HHM65_00810) comGF 129942..130382 (+) 441 WP_170078125.1 competence type IV pilus minor pilin ComGF -
  HHM65_RS00810 (HHM65_00815) comGG 130405..130734 (+) 330 WP_003058539.1 competence type IV pilus minor pilin ComGG -
  HHM65_RS00815 (HHM65_00820) comYH 130797..131750 (+) 954 WP_003060701.1 class I SAM-dependent methyltransferase Machinery gene
  HHM65_RS00820 (HHM65_00825) - 131809..133005 (+) 1197 WP_015057203.1 acetate kinase -
  HHM65_RS00825 (HHM65_00830) - 133311..133517 (+) 207 WP_003058508.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 343 a.a.        Molecular weight: 38953.34 Da        Isoelectric Point: 9.2055

>NTDB_id=439869 HHM65_RS00785 WP_015057201.1 127946..128977(+) (comYB) [Streptococcus dysgalactiae subsp. equisimilis strain TPCH-A74]
MTFLQRDISVRSKQGLKKLSSKEQYKLIQLLANLLLTGFNLAEIIAFLTKSHLIPEPYVLRMEEALLKGRGLADMLAGIG
FSDAILTQISLADGHGNIETTLVNIQGYLNQMAKLKRKTIEVVTYPIILLVFLFVMILGLRHYVMPQLEAQNQLTIFLTH
FPAIFFGIILFIGVLLGGIWLHWRSQSRLKLFTHFSAYPVLGKLLQQYLTAYYAREWGTLIGQGLELPVILDIMAMEKSL
FMQELAEDIRVSLLAGQAFHDKVATYPFFKKELSLMITYGEIKSKLGSELEIYAQESWTQFFSQLHQATQFIQPIIFLVI
ALTIVMIYAAILLPIYQNMGGVL

Nucleotide


Download         Length: 1032 bp        

>NTDB_id=439869 HHM65_RS00785 WP_015057201.1 127946..128977(+) (comYB) [Streptococcus dysgalactiae subsp. equisimilis strain TPCH-A74]
ATGACCTTCTTGCAGCGGGACATCTCAGTCCGGAGCAAGCAAGGTTTGAAAAAATTATCCAGTAAGGAACAATACAAACT
AATTCAGCTATTAGCTAACCTTTTGTTAACAGGATTTAACTTGGCTGAGATAATAGCTTTCTTAACAAAAAGTCATTTGA
TTCCCGAGCCCTATGTTCTCCGCATGGAAGAAGCACTCTTAAAAGGTCGAGGGCTGGCAGATATGTTAGCTGGGATAGGT
TTTTCGGATGCTATTTTAACTCAAATTAGTTTAGCAGATGGTCACGGAAACATTGAGACGACGCTTGTTAATATTCAGGG
CTATCTCAACCAAATGGCTAAACTTAAACGAAAAACCATTGAAGTCGTCACCTATCCGATTATTCTCCTGGTATTTCTTT
TTGTGATGATATTAGGGTTAAGGCATTATGTTATGCCTCAATTAGAAGCTCAAAATCAGCTTACTATTTTTCTGACTCAT
TTTCCAGCTATTTTCTTTGGTATTATCCTTTTTATAGGTGTGCTGTTAGGGGGAATTTGGCTGCATTGGCGATCGCAAAG
TCGCCTCAAGCTTTTTACTCACTTCAGTGCTTATCCTGTTTTAGGGAAACTCTTACAACAGTATCTGACGGCTTATTATG
CTAGAGAATGGGGAACATTGATTGGACAGGGACTTGAATTACCAGTCATTCTGGATATTATGGCCATGGAGAAATCTTTA
TTCATGCAAGAATTGGCTGAAGATATTCGAGTTAGTTTATTGGCAGGACAAGCTTTTCATGATAAGGTAGCTACCTATCC
TTTCTTTAAAAAAGAGTTAAGTTTGATGATTACTTATGGAGAGATTAAATCCAAGTTAGGAAGTGAACTAGAGATTTATG
CTCAAGAGAGTTGGACTCAGTTTTTCAGCCAACTTCATCAAGCAACTCAGTTTATTCAACCGATTATCTTTTTAGTGATT
GCTCTGACGATTGTCATGATTTATGCAGCAATTTTATTACCAATTTATCAAAATATGGGAGGCGTTTTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(28-146)

Type II secretion system (T2SS), protein F (T2SSF)

(214-334)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.392

98.834

0.528
  comYB Streptococcus mutans UA159

53.392

98.834

0.528
  comYB Streptococcus gordonii str. Challis substr. CH1

48.673

98.834

0.481
  comGB/cglB Streptococcus mitis NCTC 12261

45.97

97.668

0.449
  comGB/cglB Streptococcus mitis SK321

45.345

97.085

0.44
  comGB/cglB Streptococcus pneumoniae Rx1

43.844

97.085

0.426
  comGB/cglB Streptococcus pneumoniae D39

43.844

97.085

0.426
  comGB/cglB Streptococcus pneumoniae R6

43.844

97.085

0.426
  comGB/cglB Streptococcus pneumoniae TIGR4

43.844

97.085

0.426
  comGB Lactococcus lactis subsp. cremoris KW2

42.773

98.834

0.423