Detailed information NTG0439490

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: HG420_RS12445
• Genome accession: NZ_CP051518
• Coordinates: 1634842..1635627 (-)
• Length: 261 a.a.
• NCBI ID: WP_228763967.1
• Uniprot ID: A0AAJ6MIS5
• Organism: Lactococcus cremoris strain F
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1629842..1640627

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HG420_RS08210 (HG420_08220)	-	1630495..1631448 (+)	954	WP_015082369.1	PhoH family protein	-
HG420_RS08215 (HG420_08225)	-	1631518..1632012 (+)	495	WP_015082370.1	NUDIX hydrolase	-
HG420_RS08220 (HG420_08230)	ybeY	1632103..1632591 (+)	489	WP_011835335.1	rRNA maturation RNase YbeY	-
HG420_RS08225 (HG420_08235)	-	1632575..1633030 (+)	456	WP_015082371.1	diacylglycerol kinase	-
HG420_RS08230 (HG420_08240)	-	1633047..1633625 (+)	579	WP_015082372.1	nucleotidyltransferase family protein	-
HG420_RS08235 (HG420_08245)	comFC	1633683..1634333 (-)	651	WP_015082373.1	ComF family protein	Machinery gene
HG420_RS12440	comFA	1634333..1634845 (-)	513	WP_228763816.1	helicase-related protein	Machinery gene
HG420_RS12445	comFA	1634842..1635627 (-)	786	WP_228763967.1	DEAD/DEAH box helicase family protein	Machinery gene
HG420_RS08245 (HG420_08255)	-	1635684..1636319 (+)	636	WP_015082374.1	YigZ family protein	-
HG420_RS08250 (HG420_08260)	-	1636361..1636861 (+)	501	WP_168784118.1	VanZ family protein	-
HG420_RS08255 (HG420_08265)	-	1636948..1637946 (+)	999	WP_237025417.1	DUF475 domain-containing protein	-
HG420_RS08260 (HG420_08270)	-	1638055..1638822 (+)	768	WP_011676099.1	5'-nucleotidase	-
HG420_RS08265 (HG420_08275)	-	1639059..1639706 (+)	648	WP_011676100.1	GIY-YIG nuclease family protein	-

Sequence

Protein

• Length: 261 a.a.
• Molecular weight: 29903.55 Da
• Isoelectric Point: 7.8602

>NTDB_id=439490 HG420_RS12445 WP_228763967.1 1634842..1635627(-) (comFA) [Lactococcus cremoris strain F]
MNLILENLLGRLLLEKDISAFYNFTDQVNNENKLIKICAMTSVNANKVRCNRCGTIHIKTNVKLPIGAFFCPTCLELGRV
RSDEYFYHLPQQDFSEKTYLRWTGKLTENQEKISNALCQQITNNQKRLVQAVTGAGKTEMIYQLIEQILSHGGSVGLASP
RIDVCIELHQRLSRDFTCQIPLLYHEGDSYFRSPLVVMTSHQLLRFKEAFDLLIIDEVDAFPFRDNDMLFFALENAKKIN
GNLLYLTATSTDKLDKQIKKA

Nucleotide

• Length: 786 bp

>NTDB_id=439490 HG420_RS12445 WP_228763967.1 1634842..1635627(-) (comFA) [Lactococcus cremoris strain F]
ATGAACTTAATTTTAGAAAATCTCCTTGGGCGTCTTTTACTAGAAAAAGATATTTCAGCTTTTTATAATTTTACTGACCA
AGTTAACAATGAAAACAAATTAATTAAAATTTGTGCAATGACATCTGTCAATGCTAATAAAGTAAGATGTAATCGATGTG
GCACGATTCATATAAAAACCAATGTTAAACTTCCGATTGGTGCTTTTTTCTGTCCAACATGTCTTGAACTTGGTCGTGTC
CGCTCAGATGAATATTTCTATCATCTCCCTCAACAAGACTTTTCAGAAAAAACTTACTTACGTTGGACAGGAAAATTAAC
AGAAAATCAAGAAAAAATTTCTAACGCTCTCTGTCAGCAAATTACTAATAATCAAAAACGACTTGTTCAAGCCGTTACTG
GTGCTGGTAAAACAGAAATGATATATCAGCTTATTGAACAAATTCTTAGCCATGGGGGTTCGGTCGGTTTAGCCAGCCCA
AGAATTGATGTATGCATTGAGCTTCATCAACGTTTATCACGTGATTTCACTTGTCAAATTCCCCTTTTGTATCATGAGGG
AGATTCTTATTTTCGTTCGCCTTTGGTCGTTATGACCAGTCACCAACTTTTACGTTTCAAAGAGGCCTTTGATTTGCTTA
TCATCGATGAAGTCGATGCCTTTCCTTTTAGAGATAATGACATGCTTTTTTTCGCTCTTGAAAATGCTAAAAAAATTAAT
GGAAACTTGCTCTACTTAACCGCTACATCTACTGACAAATTAGACAAACAGATAAAAAAAGCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (105-249)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	96.923	99.617	0.966
comFA/cflA	Streptococcus mitis SK321	49.407	96.935	0.479
comFA/cflA	Streptococcus pneumoniae D39	48.617	96.935	0.471
comFA/cflA	Streptococcus pneumoniae Rx1	48.617	96.935	0.471
comFA/cflA	Streptococcus pneumoniae R6	48.617	96.935	0.471
comFA/cflA	Streptococcus pneumoniae TIGR4	48.617	96.935	0.471
comFA/cflA	Streptococcus mitis NCTC 12261	48.221	96.935	0.467
comFA	Bacillus subtilis subsp. subtilis str. 168	37.453	100	0.383