Detailed information NTG0439098

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: IPV40_RS02190
• Genome accession: NZ_CP063394
• Coordinates: 481991..483139 (+)
• Length: 382 a.a.
• NCBI ID: WP_003100959.1
• Uniprot ID: G3XCX3
• Organism: Pseudomonas aeruginosa strain ST167_d67burn1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 476991..488139

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
IPV40_RS02165 (IPV40_02165)	-	478142..478438 (-)	297	WP_031628111.1	DUF167 domain-containing protein	-
IPV40_RS02170 (IPV40_02170)	-	478435..479028 (-)	594	WP_003084544.1	YggT family protein	-
IPV40_RS02175 (IPV40_02175)	proC	479039..479860 (-)	822	WP_003142635.1	pyrroline-5-carboxylate reductase	-
IPV40_RS02180 (IPV40_02180)	-	479872..480564 (-)	693	WP_003084549.1	YggS family pyridoxal phosphate-dependent enzyme	-
IPV40_RS02185 (IPV40_02185)	pilT	480779..481813 (+)	1035	WP_003084552.1	type IV pilus twitching motility protein PilT	Machinery gene
IPV40_RS02190 (IPV40_02190)	pilU	481991..483139 (+)	1149	WP_003100959.1	type IV pilus ATPase PilU	Machinery gene
IPV40_RS02195 (IPV40_02195)	czcD	483147..484046 (-)	900	WP_003100957.1	CDF family zinc transporter CzcD	-
IPV40_RS02200 (IPV40_02200)	-	484200..484604 (+)	405	WP_003084560.1	NINE protein	-
IPV40_RS02205 (IPV40_02205)	-	484847..486220 (+)	1374	WP_015502220.1	pyridoxal-phosphate dependent enzyme	-
IPV40_RS02210 (IPV40_02210)	-	486217..487401 (+)	1185	WP_023086800.1	cystathionine gamma-synthase	-

Sequence

Protein

• Length: 382 a.a.
• Molecular weight: 42532.81 Da
• Isoelectric Point: 6.7792

>NTDB_id=439098 IPV40_RS02190 WP_003100959.1 481991..483139(+) (pilU) [Pseudomonas aeruginosa strain ST167_d67burn1]
MEFEKLLRLMVEKGGSDLFITAGVPPSMKVNGRVMPVTKTPLSPEQTRETVLGVMNEQQRRDFAENHECNFAISARGIGR
FRVSAFYQRNLVGMVLRRIETNIPTLEELKLPEILKKLALTKRGLVIFVGATGTGKSTSLAAMIGYRNKNSTGHIISIED
PIEYIHQHQGCIVTQREVGLDTDSFEVALKNTLRQAPDVIMIGEVRSRETMDHAVAFAETGHLCLATLHANNANQALERI
IHFFPADRHGQVWMDLSLNLKAIVAQQLVPTPDGKGRRAVIEVLLNTPLAADLIRKGEVHELKPLMKRSTEQGMQTFDQA
LYQLYTQGEITYEDALAHADSANDLRLMIKLGSESDADHLSSLTQGLSLEITDDDPAGRRFR

Nucleotide

• Length: 1149 bp

>NTDB_id=439098 IPV40_RS02190 WP_003100959.1 481991..483139(+) (pilU) [Pseudomonas aeruginosa strain ST167_d67burn1]
ATGGAATTCGAAAAGCTGCTGCGCCTGATGGTGGAAAAGGGCGGCTCCGACCTGTTCATCACCGCCGGCGTACCGCCGTC
GATGAAGGTCAACGGCCGGGTGATGCCGGTGACCAAGACGCCGCTGTCGCCGGAGCAGACCCGCGAGACCGTGCTAGGCG
TGATGAACGAGCAGCAGCGCCGCGATTTCGCCGAGAACCATGAGTGCAACTTCGCCATCAGCGCCCGCGGCATCGGCCGC
TTCCGGGTCAGTGCCTTCTACCAGCGCAACCTGGTGGGCATGGTATTGCGCCGGATCGAGACCAACATCCCGACCCTGGA
AGAGCTGAAGCTCCCGGAAATCCTCAAGAAGCTGGCGCTGACCAAGCGCGGCCTGGTGATCTTCGTCGGCGCCACCGGCA
CCGGCAAGTCCACCTCGCTGGCGGCGATGATCGGCTACCGCAACAAGAACTCCACCGGGCACATCATCTCCATCGAGGAC
CCGATCGAGTACATCCACCAGCACCAGGGCTGCATCGTCACCCAGCGCGAGGTGGGGCTGGACACCGACTCCTTCGAGGT
GGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCTCGCGGGAGACCATGGACCACG
CCGTGGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCGACCCTGCACGCGAACAACGCCAACCAGGCGCTGGAGCGGATC
ATCCACTTCTTCCCGGCCGACCGTCATGGCCAGGTGTGGATGGACCTGTCGCTGAACCTCAAGGCCATCGTCGCCCAGCA
ACTGGTGCCGACCCCCGACGGCAAGGGGCGGCGGGCGGTGATCGAGGTGCTGCTGAACACCCCGCTGGCCGCCGACCTGA
TCCGCAAGGGCGAGGTGCACGAGCTGAAGCCGCTGATGAAGCGCTCCACCGAGCAGGGCATGCAGACCTTCGACCAGGCG
CTGTACCAGCTCTACACCCAGGGCGAGATCACCTACGAGGACGCCCTGGCCCACGCCGACTCGGCCAACGACCTGCGTCT
GATGATCAAGCTCGGTTCGGAAAGCGACGCCGACCACCTGTCGAGCCTGACCCAGGGCCTGAGCCTGGAAATCACCGACG
ACGACCCTGCCGGCCGGCGCTTCCGCTGA

Domains

Predicted by InterProScan.

• Type II/IV secretion system protein (T2SSE) (111-270)

3D structure

• AlphaFold DB: G3XCX3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	88.482	100	0.885
pilU	Acinetobacter baylyi ADP1	65.266	93.455	0.61
pilU	Vibrio cholerae strain A1552	60.286	91.623	0.552
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.068	88.743	0.382