Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   IL987_RS13620 Genome accession   NZ_CP063214
Coordinates   2771251..2772489 (-) Length   412 a.a.
NCBI ID   WP_000816005.1    Uniprot ID   B7UK96
Organism   Escherichia coli strain ERP001     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2766251..2777489
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IL987_RS13600 dam 2766863..2767699 (-) 837 WP_000742141.1 adenine-specific DNA-methyltransferase -
  IL987_RS13605 damX 2767806..2769092 (-) 1287 WP_000343172.1 cell division protein DamX -
  IL987_RS13610 aroB 2769184..2770272 (-) 1089 WP_000439850.1 3-dehydroquinate synthase -
  IL987_RS13615 aroK 2770329..2770850 (-) 522 WP_000818618.1 shikimate kinase AroK -
  IL987_RS13620 comE 2771251..2772489 (-) 1239 WP_000816005.1 DNA uptake porin HofQ Machinery gene
  IL987_RS13625 hofP 2772401..2772805 (-) 405 WP_001264138.1 DNA utilization protein HofP -
  IL987_RS13630 hofO 2772795..2773235 (-) 441 WP_001055747.1 DNA utilization protein HofO -
  IL987_RS13635 hofN 2773219..2773758 (-) 540 WP_001069330.1 DNA utilization protein HofN -
  IL987_RS13640 hofM 2773758..2774537 (-) 780 WP_001296474.1 DNA utilization protein HofM -
  IL987_RS13645 mrcA 2774657..2777209 (+) 2553 WP_193579143.1 peptidoglycan glycosyltransferase/peptidoglycan DD-transpeptidase MrcA -

Sequence

Protein


Download         Length: 412 a.a.        Molecular weight: 44811.29 Da        Isoelectric Point: 5.9488

>NTDB_id=437553 IL987_RS13620 WP_000816005.1 2771251..2772489(-) (comE) [Escherichia coli strain ERP001]
MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQEKLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGL
ITRQEGNILSVHSVAWQNDNIARQEAEQTRAQANLPLENRNITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
KTALSTLEQWVSQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQQAGGVGQVTTLGSDLSVATATTHIGFNIGRIN
GRLLDLELSALEQKQQLDIIASPRLLASHLQPASIKQGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKL
HISQNVPGQVLQQADGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDGKEDERREL
VVFITPRLVSSE

Nucleotide


Download         Length: 1239 bp        

>NTDB_id=437553 IL987_RS13620 WP_000816005.1 2771251..2772489(-) (comE) [Escherichia coli strain ERP001]
ATGAAGCAATGGATAGCCGCACTACTGTTGATGCTTATACCCGGCGTACAGGCGGCAAAGCCGCAAAAAGTGACGCTGAT
GGTGGATGACGTTCCGGTAGCTCAGGTGTTGCAGGCGCTGGCTGAACAGGAGAAGTTGAACCTGGTGGTTTCGCCAGACG
TCAGCGGTACGGTGTCGTTACATTTAACTGACGTTCCCTGGAAGCAGGCACTACAAACTGTAGTGAAAAGCGCCGGATTG
ATAACGCGCCAGGAGGGCAACATTCTCTCGGTGCATTCCGTTGCCTGGCAGAATGACAATATCGCCCGCCAGGAGGCGGA
GCAGACGCGGGCGCAGGCAAATCTGCCGCTGGAAAATCGCAATATTACTCTGCAATACGCCGACGCCGGAGAGCTGGCGA
AAGCGGGGGAGAAGCTACTGAGTGCCAAAGGGAGTATGACCGTCGATAAACGCACCAATCGCCTTTTGCTGCGAGATAAC
AAAACGGCGTTAAGCACGCTTGAACAGTGGGTATCGCAAATGGATCTGCCGGTCGGGCAGGTTGAGCTCTCGGCGCATAT
TGTCACCATTAATGAAAAAAGTTTGCGTGAGTTAGGTGTGAAATGGACGCTGGCCGATGCGCAACAAGCTGGTGGCGTTG
GGCAAGTCACCACGCTTGGCAGCGACCTCTCCGTAGCGACGGCGACAACGCATATCGGTTTTAACATTGGACGCATCAAC
GGACGTTTACTGGATCTTGAGCTTTCCGCGCTCGAACAAAAACAGCAGCTGGATATTATCGCCAGTCCGCGTCTGCTGGC
CTCACATCTTCAGCCTGCCAGCATTAAACAGGGGAGCGAAATTCCATATCAGGTTTCCAGCGGGGAAAGTGGCGCGACGT
CGGTGGAATTTAAAGAGGCCGTCCTGGGGATGGAAGTCACGCCCACGGTGTTACAAAAAGGTCGTATCCGGCTGAAATTA
CACATCAGCCAGAACGTTCCGGGGCAGGTGCTACAGCAGGCAGATGGCGAAGTGCTGGCGATTGATAAGCAGGAGATCGA
AACGCAGGTCGAGGTCAAAAGCGGAGAAACGTTGGCGCTGGGCGGCATTTTTACCCGTAAAAATAAATCGGGTCAGGATA
GCGTACCGTTGCTTGGCGACATTCCCTGGTTCGGGCAATTATTTCGTCATGACGGAAAAGAAGATGAACGACGCGAGTTA
GTGGTGTTTATCACGCCACGACTGGTTTCCAGTGAGTAA

Domains

Predicted by InterProScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(120-179)

Bacterial type II and III secretion system protein (Secretin)

(250-409)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB B7UK96

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae 86-028NP

37.3

100

0.396
  pilQ Vibrio campbellii strain DS40M4

38.48

100

0.393
  comE Haemophilus influenzae Rd KW20

37.156

100

0.393
  pilQ Vibrio cholerae strain A1552

37.772

100

0.379
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

37.772

100

0.379
  pilQ Pseudomonas aeruginosa PAK

34.633

100

0.367
  comE Glaesserella parasuis strain SC1401

35.714

100

0.364