Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PSF113_RS32435 Genome accession   NC_016830
Coordinates   503236..504300 (+) Length   354 a.a.
NCBI ID   WP_014336162.1    Uniprot ID   -
Organism   Pseudomonas ogarae     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 498236..509300
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSF113_RS32420 (PSF113_0420) rpmE 498467..498691 (+) 225 WP_014336159.1 50S ribosomal protein L31 -
  PSF113_RS32425 (PSF113_0421) - 499014..500282 (+) 1269 WP_014336160.1 malic enzyme-like NAD(P)-binding protein -
  PSF113_RS32430 (PSF113_0422) - 500584..503028 (-) 2445 WP_373419637.1 penicillin-binding protein 1A -
  PSF113_RS32435 (PSF113_0423) comM 503236..504300 (+) 1065 WP_014336162.1 pilus assembly protein PilM Machinery gene
  PSF113_RS32440 (PSF113_0424) - 504300..504866 (+) 567 WP_014336163.1 PilN domain-containing protein -
  PSF113_RS32445 (PSF113_0425) pilO 504863..505486 (+) 624 WP_014336164.1 type 4a pilus biogenesis protein PilO -
  PSF113_RS32450 (PSF113_0426) - 505483..506010 (+) 528 WP_014336165.1 pilus assembly protein PilP -
  PSF113_RS32455 (PSF113_0427) pilQ 506024..508111 (+) 2088 WP_014336166.1 type IV pilus secretin PilQ Machinery gene
  PSF113_RS32460 (PSF113_0428) aroK 508116..508634 (+) 519 WP_003196983.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38139.70 Da        Isoelectric Point: 4.9112

>NTDB_id=43735 PSF113_RS32435 WP_014336162.1 503236..504300(+) (comM) [Pseudomonas ogarae]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKARTPLRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVVGVSPRSTERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=43735 PSF113_RS32435 WP_014336162.1 503236..504300(+) (comM) [Pseudomonas ogarae]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTCGAGTT
GAGCCGCCAGGGTGAACGCTACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCAGCCAACGCCGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGCCAGGCGTTGTCGCGTCTGTTGGCCAAGGCCAGGACGCCCTTGCGTAGCGTGGCGGTG
GCCGTGGCAGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGCTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATATATTCCCTATCCCCTGGATGAGGTGGCCATTGATTTCGAAGTGGTGGGTGTTTCGCCAC
GCAGTACCGAGCGCGTCGAAGTGCTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGCGCGTTCGGCCTGCTCGCCACGCAACTGGC
TGCCTCCCAGGAACGGCTGACCGTGGCAGTTGTCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCATAACGGGC
GCATCATCTATACCCGCGAACAGTTGTTCGGCGGCCGCCAGCTGACCGAGGAAATTCAGCGCCGCTATGGCCTGACACCC
GAGCAGGCGGGCCTGGCAAAGAAGCAGGGTGGTCTGCCCGACGATTATCTCAGCGAGGTGCTGCAACCTTTTCGCGAGGC
CCTGGTACAGCAGGTGTCGCGGTCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGCGCGGTGGACCACATCCTGCTGG
CTGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGTGCCCTGGCCAGTGATGCGCCAGCACTGATGATTGCTTGTGG
GCTGGCCCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.52

100

0.545
  pilM Legionella pneumophila strain ERS1305867

47.74

100

0.477

Multiple sequence alignment