Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PSF113_RS32455 Genome accession   NC_016830
Coordinates   506024..508111 (+) Length   695 a.a.
NCBI ID   WP_014336166.1    Uniprot ID   -
Organism   Pseudomonas ogarae     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 501024..513111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSF113_RS32435 (PSF113_0423) comM 503236..504300 (+) 1065 WP_014336162.1 pilus assembly protein PilM Machinery gene
  PSF113_RS32440 (PSF113_0424) - 504300..504866 (+) 567 WP_014336163.1 PilN domain-containing protein -
  PSF113_RS32445 (PSF113_0425) pilO 504863..505486 (+) 624 WP_014336164.1 type 4a pilus biogenesis protein PilO -
  PSF113_RS32450 (PSF113_0426) - 505483..506010 (+) 528 WP_014336165.1 pilus assembly protein PilP -
  PSF113_RS32455 (PSF113_0427) pilQ 506024..508111 (+) 2088 WP_014336166.1 type IV pilus secretin PilQ Machinery gene
  PSF113_RS32460 (PSF113_0428) aroK 508116..508634 (+) 519 WP_003196983.1 shikimate kinase AroK -
  PSF113_RS32465 (PSF113_0429) aroB 508783..509883 (+) 1101 WP_014336168.1 3-dehydroquinate synthase -
  PSF113_RS32470 (PSF113_0430) - 509894..511480 (+) 1587 WP_014336169.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75008.03 Da        Isoelectric Point: 5.2895

>NTDB_id=43736 PSF113_RS32455 WP_014336166.1 506024..508111(+) (pilQ) [Pseudomonas ogarae]
MNRFFSALGISLWIALLSPMVHGASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLASKRLD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRVEGNNLFVVVGKGSAPRSAAPKSSAQAQRPAVASAAPARTSAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWSSSG
VTTGDSTTIGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSDSKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=43736 PSF113_RS32455 WP_014336166.1 506024..508111(+) (pilQ) [Pseudomonas ogarae]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCATGGGGCCAGTCTGAA
GACGCTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCACCTTCGACGGGCCGCCGCCGACACCCCGGG
GCTACACCACCGAACAACCGGCGCGGATTGCACTGGACCTGCCGGGCGTGACCAATCAGTTGGCCAGCAAGCGCCTCGAC
CTGGGCGGGGGCAACGCGCGCAGCGCCACCGTGGTCGAGGCGGGGGATCGCACCCGGTTGATCATCGGCCTTACCCAACT
GGCGCCGTATGACACCCGGGTCGAAGGGAATAACCTGTTCGTGGTGGTTGGCAAGGGCTCCGCCCCAAGGAGTGCTGCCC
CGAAGAGCTCCGCCCAGGCCCAGCGCCCGGCGGTTGCCAGCGCTGCGCCGGCGAGGACCAGTGCGCCGGTGGGCAAGGCG
ATCCGTGGGGTGGACTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGATCTGTCTGATCCGTCCATCGCCCC
GGATATCCAGGAGCGCGAAGGCAAGATCATCCTAAATTTTGCCAGGACCCAGTTGCCAGAGCCGCTGCGGGTGCGCCTGG
ATGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGTATCGAACCC
AGTGGGACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGACCT
GCAGAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAACTCTCACTCAACTTCCAGGACATCGAGGTGCGTTCGG
TCCTGCAACTGATTGCCGATTTTACCAACCTGAACCTTGTGGCCAGCGATACGGTGCAGGGTGGCATTACCCTGCGGTTG
CAGAATGTGCCTTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTTGGCAACGTGCT
GTTGGTGGCTCCGGCCGATGAAATTGCCGCCCGTGAGCGCCAGGAACTGGAGTCGCAGAAACAGATTGCCGACCTGGCGC
CGCTGCGGCGTGAGCTGTTGCAGGTCAATTATGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCC
GAGGCAAAAGCCGATGAGCGCGGCACGATCACGGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACCCAGGACCG
TCTCGATGAACTGCGCCGGATCGTGGCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAGG
CTAACGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGTTCGATTCAAAACAAAGGCAACTGGAGTTCCTCGGGG
GTCACCACGGGCGACTCCACCACCATCGGCACGCCGGGCAGCACCAGTACCAACCAGCCGTTCGTCGACCTGGGCGCCTC
GGGCAATACCTCGGGCATCGGCATTGCCTTCATTACCGACAACGTATTGTTGGACCTTGAGCTGACGGCCATGGAGAAAA
CCGGCAACGGGGAAATCGTCTCCCAGCCCAAGGTGGTGACGTCTGACAAAGAGACGGCAAAGATCCTCAAGGGTACGGAA
ATTCCTTATCAGGAAGCCAGTTCCAGCGGTGCTACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGACTCC
GCAGATCACCCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGAATG
ATGTGCCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGTGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGATAGTAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.093

100

0.758
  comQ Acinetobacter baylyi ADP1

41.151

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.389

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.389

100

0.412
  pilQ Legionella pneumophila strain ERS1305867

37.85

97.698

0.37
  pilQ Legionella pneumophila strain Lp02

37.85

97.698

0.37

Multiple sequence alignment