Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   IMY14_RS13740 Genome accession   NZ_CP063150
Coordinates   2632661..2633071 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain s-16     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2627661..2638071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMY14_RS13710 - 2627748..2627888 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  IMY14_RS13715 yqeF 2628194..2628925 (-) 732 WP_047182903.1 SGNH/GDSL hydrolase family protein -
  IMY14_RS13720 cwlH 2629177..2629929 (-) 753 WP_047182904.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  IMY14_RS13725 yqeD 2630116..2630742 (+) 627 WP_047182905.1 TVP38/TMEM64 family protein -
  IMY14_RS13730 gnd 2630761..2631654 (-) 894 WP_047182906.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  IMY14_RS13735 yqeB 2631906..2632628 (+) 723 WP_047182907.1 hypothetical protein -
  IMY14_RS13740 nucA/comI 2632661..2633071 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  IMY14_RS13745 - 2633267..2633614 (+) 348 Protein_2691 sigma-70 family RNA polymerase sigma factor -
  IMY14_RS13750 spoIVCA 2633645..2635105 (-) 1461 WP_253206441.1 site-specific DNA recombinase SpoIVCA -
  IMY14_RS21995 - 2635063..2635241 (-) 179 Protein_2693 hypothetical protein -
  IMY14_RS13755 mauJ 2635543..2637069 (+) 1527 WP_174616725.1 methylamine utilization protein MauJ -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=437127 IMY14_RS13740 WP_009967785.1 2632661..2633071(-) (nucA/comI) [Bacillus subtilis strain s-16]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=437127 IMY14_RS13740 WP_009967785.1 2632661..2633071(-) (nucA/comI) [Bacillus subtilis strain s-16]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCTCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGAGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGTGAGGAAGGCGGCGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTATCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAG

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529