Detailed information NTG0436706

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HF888_RS15305
• Genome accession: NZ_CP051183
• Coordinates: 3281597..3282661 (-)
• Length: 354 a.a.
• NCBI ID: WP_007018236.1
• Uniprot ID: Q1N2I0
• Organism: Bermanella marisrubri strain RED65
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3276597..3287661

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HF888_RS15280 (HF888_15280)	aroK	3277238..3277765 (-)	528	WP_007018231.1	shikimate kinase AroK	-
HF888_RS15285 (HF888_15285)	pilQ	3277775..3279865 (-)	2091	WP_007018232.1	type IV pilus secretin PilQ family protein	Machinery gene
HF888_RS15290 (HF888_15290)	-	3279866..3280402 (-)	537	WP_007018233.1	pilus assembly protein PilP	-
HF888_RS15295 (HF888_15295)	pilO	3280399..3281046 (-)	648	WP_007018234.1	type 4a pilus biogenesis protein PilO	-
HF888_RS15300 (HF888_15300)	-	3281046..3281597 (-)	552	WP_007018235.1	PilN domain-containing protein	-
HF888_RS15305 (HF888_15305)	pilM	3281597..3282661 (-)	1065	WP_007018236.1	pilus assembly protein PilM	Machinery gene
HF888_RS15310 (HF888_15310)	-	3282817..3285441 (+)	2625	WP_133308492.1	penicillin-binding protein 1A	-
HF888_RS15315 (HF888_15315)	-	3285433..3286506 (-)	1074	WP_007018238.1	hypothetical protein	-
HF888_RS15320 (HF888_15320)	dtd	3286591..3287025 (-)	435	WP_007018239.1	D-aminoacyl-tRNA deacylase	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38704.81 Da
• Isoelectric Point: 4.1757

>NTDB_id=436706 HF888_RS15305 WP_007018236.1 3281597..3282661(-) (pilM) [Bermanella marisrubri strain RED65]
MLANLFKKKNKAVLGVDISSTRVKLIELTKNNDRMQVEAYASEILPENAVVEQSISDEEVVGGVIKKALTRSRSTCKRGA
VAVAGSAVITKTIQMAADMNDDERENQIKVEADQYIPYPLEEVAMDFEVQGPVEGNEQQVDVLLAACRKETVELREDSIE
IGGLESAVVDVEAYCIERAYQLLEPQLEGDNIDTVAIIDIGATMTTLNVLHQGSIIYNREQMFGGQQLTEEIQRRYGISA
EEATRAKLEGGLPDDYETEILLPFKESVVQQVSRSLQFFYSSSQYNDVDYVILAGGTSSLDGLAQLVQEKIGTPALIANP
FANMTVGPKVNAQMLSNDAPGLLVACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=436706 HF888_RS15305 WP_007018236.1 3281597..3282661(-) (pilM) [Bermanella marisrubri strain RED65]
GTGCTAGCCAATTTATTTAAGAAAAAAAATAAGGCAGTTCTTGGTGTGGATATCAGCTCTACACGCGTCAAATTGATTGA
ACTGACAAAAAATAATGATCGCATGCAAGTCGAGGCCTATGCGTCGGAAATCTTGCCAGAAAATGCAGTAGTAGAACAAA
GTATTAGTGACGAAGAGGTTGTTGGCGGGGTCATCAAAAAAGCATTGACCCGATCGCGCTCAACCTGCAAGCGCGGCGCT
GTTGCTGTTGCCGGATCGGCTGTTATTACCAAGACCATCCAGATGGCCGCGGATATGAACGACGATGAGCGCGAAAACCA
AATCAAAGTTGAAGCCGATCAATATATCCCTTACCCACTTGAAGAAGTGGCCATGGATTTTGAGGTTCAAGGTCCTGTTG
AAGGTAATGAACAACAAGTGGATGTTTTGTTGGCTGCTTGCCGAAAAGAAACAGTTGAGCTTCGTGAAGATTCGATCGAA
ATCGGTGGTCTTGAATCCGCAGTAGTTGACGTAGAGGCCTACTGTATCGAACGTGCCTATCAATTATTGGAGCCGCAGCT
TGAAGGTGACAACATTGATACAGTCGCTATCATCGATATTGGTGCGACTATGACGACACTCAACGTGTTGCACCAAGGCA
GTATTATCTACAATCGAGAACAAATGTTTGGTGGTCAGCAGTTAACTGAAGAAATTCAGCGGCGCTACGGTATCTCTGCG
GAAGAAGCCACGCGAGCTAAGTTAGAAGGTGGCTTGCCTGATGACTATGAAACGGAAATTTTACTGCCTTTTAAAGAGTC
GGTTGTTCAGCAGGTCAGTCGCTCATTGCAGTTCTTTTATAGTTCTAGTCAGTACAATGATGTGGATTACGTGATTCTAG
CGGGTGGAACGAGCTCCCTTGATGGCTTGGCCCAGTTGGTTCAAGAAAAGATAGGCACACCTGCATTAATAGCCAACCCC
TTTGCCAATATGACGGTAGGCCCCAAAGTGAATGCTCAAATGTTATCCAATGATGCTCCTGGTTTGTTAGTGGCGTGTGG
TTTAGCCCTGAGGAGCTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (15-353)

3D structure

• AlphaFold DB: Q1N2I0

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.276	99.153	0.528
comM	Acinetobacter nosocomialis M2	53.276	99.153	0.528
comM	Acinetobacter baylyi ADP1	48.442	99.718	0.483
pilM	Legionella pneumophila strain ERS1305867	43.785	100	0.438