Detailed information NTG0436543

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: HF909_RS15055
• Genome accession: NZ_CP051169
• Coordinates: 3115731..3116252 (-)
• Length: 173 a.a.
• NCBI ID: WP_193026452.1
• Uniprot ID: -
• Organism: Ralstonia pseudosolanacearum strain RUN2474
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3105677..3116252	3115731..3116252	within	0

Gene organization within MGE regions

Location: 3105677..3116252

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HF909_RS26820	-	3105848..3106138 (-)	291	Protein_2945	helix-turn-helix domain-containing protein	-
HF909_RS15005 (HF909_15100)	-	3106359..3107288 (+)	930	WP_020749589.1	branched-chain amino acid transaminase	-
HF909_RS15010 (HF909_15105)	-	3107313..3107510 (+)	198	WP_011000524.1	zinc-finger domain-containing protein	-
HF909_RS15015 (HF909_15110)	waaF	3107560..3108585 (+)	1026	WP_020749591.1	lipopolysaccharide heptosyltransferase II	-
HF909_RS15020 (HF909_15115)	-	3108625..3109065 (+)	441	WP_003278969.1	nuclear transport factor 2 family protein	-
HF909_RS15025 (HF909_15120)	-	3109105..3110220 (+)	1116	WP_193026447.1	YheT family hydrolase	-
HF909_RS15030 (HF909_15125)	-	3110213..3110800 (+)	588	WP_193026448.1	DUF2946 family protein	-
HF909_RS15035 (HF909_15130)	-	3110815..3112503 (-)	1689	WP_193026449.1	tetratricopeptide repeat protein	-
HF909_RS15040 (HF909_15135)	moaC	3112597..3113073 (+)	477	WP_193026450.1	cyclic pyranopterin monophosphate synthase MoaC	-
HF909_RS15045 (HF909_15140)	-	3113145..3114878 (-)	1734	WP_193028552.1	PglL family O-oligosaccharyltransferase	-
HF909_RS15050 (HF909_15145)	pilA	3114952..3115458 (-)	507	WP_193026451.1	pilin	Machinery gene
HF909_RS15055 (HF909_15150)	pilA	3115731..3116252 (-)	522	WP_193026452.1	pilin	Machinery gene

Sequence

Protein

• Length: 173 a.a.
• Molecular weight: 17888.55 Da
• Isoelectric Point: 9.8138

>NTDB_id=436543 HF909_RS15055 WP_193026452.1 3115731..3116252(-) (pilA) [Ralstonia pseudosolanacearum strain RUN2474]
MASKHSRNRWRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVVEGLSLAAQAKALVVENAANAQSSLSLGSASL
PASRNVSALNIDSSTGEIAVVYASAVTGAEANTLVLTPYSGSSSSLANLQAGSAPPTMVLWVCTAAGKSMPVNTVTQRTT
ATLPPKYAPAECR

Nucleotide

• Length: 522 bp

>NTDB_id=436543 HF909_RS15055 WP_193026452.1 3115731..3116252(-) (pilA) [Ralstonia pseudosolanacearum strain RUN2474]
ATGGCATCGAAGCACAGCAGGAACAGGTGGCGCGGCCAGCGCGGCTTTACATTGATTGAATTGATGATCGTGGTGGCGAT
CATCGGGATATTGGCGGCGATTGCGATTCCGGCCTATCAGGACTACACGATCCGCGCGCGCGTGGTTGAGGGGCTGTCGC
TGGCAGCGCAGGCCAAGGCGCTGGTGGTTGAGAATGCTGCCAACGCGCAGAGTAGCCTGTCACTCGGCTCTGCATCGTTG
CCGGCCAGCAGGAACGTCAGTGCGCTGAATATTGATTCGTCCACGGGCGAAATCGCGGTGGTCTATGCATCTGCTGTTAC
GGGAGCGGAGGCGAATACGTTGGTATTGACCCCGTACTCCGGAAGCTCGTCATCACTTGCCAATCTGCAGGCGGGTTCAG
CGCCGCCGACAATGGTGCTGTGGGTGTGTACAGCCGCCGGCAAGTCGATGCCAGTCAATACTGTCACCCAGAGAACGACG
GCTACGCTGCCACCGAAGTACGCGCCCGCAGAGTGCCGTTGA

Domains

Predicted by InterproScan.

• Pilin (bacterial filament) (Pilin) (45-173)

• Prokaryotic N-terminal methylation motif (N_methyl) (10-35)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Ralstonia pseudosolanacearum GMI1000	53.977	100	0.549
pilA2	Legionella pneumophila str. Paris	46.061	95.376	0.439
pilA2	Legionella pneumophila strain ERS1305867	45.455	95.376	0.434
comP	Acinetobacter baylyi ADP1	43.478	93.064	0.405
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	35.602	100	0.393