Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   IMQ31_RS18265 Genome accession   NZ_CP062824
Coordinates   3859079..3860572 (+) Length   497 a.a.
NCBI ID   WP_024230002.1    Uniprot ID   -
Organism   Escherichia coli O167:H26 strain Res13-Lact-PER13-34     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3854079..3865572
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  IMQ31_RS18245 (IMQ31_18255) pilD 3854727..3855536 (+) 810 WP_195714851.1 prepilin peptidase PppA Machinery gene
  IMQ31_RS18250 (IMQ31_18260) gspS2 3855602..3856012 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  IMQ31_RS18255 (IMQ31_18265) gspC 3856030..3856989 (+) 960 WP_032152731.1 type II secretion system protein GspC -
  IMQ31_RS18260 (IMQ31_18270) gspD 3857019..3859079 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  IMQ31_RS18265 (IMQ31_18275) pilB/pilB1 3859079..3860572 (+) 1494 WP_024230002.1 type II secretion system ATPase GspE Machinery gene
  IMQ31_RS18270 (IMQ31_18280) gspF 3860572..3861795 (+) 1224 WP_195714852.1 type II secretion system inner membrane protein GspF -
  IMQ31_RS18275 (IMQ31_18285) gspG 3861812..3862267 (+) 456 WP_001087296.1 type II secretion system major pseudopilin GspG -
  IMQ31_RS18280 (IMQ31_18290) gspH 3862271..3862834 (+) 564 WP_001115147.1 type II secretion system minor pseudopilin GspH -
  IMQ31_RS18285 (IMQ31_18295) gspI 3862831..3863202 (+) 372 WP_001578977.1 type II secretion system minor pseudopilin GspI -
  IMQ31_RS18290 (IMQ31_18300) gspJ 3863199..3863804 (+) 606 WP_001578976.1 type II secretion system minor pseudopilin GspJ -
  IMQ31_RS18295 (IMQ31_18305) gspK 3863801..3864778 (+) 978 WP_000633199.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55173.30 Da        Isoelectric Point: 6.0074

>NTDB_id=434425 IMQ31_RS18265 WP_024230002.1 3859079..3860572(+) (pilB/pilB1) [Escherichia coli O167:H26 strain Res13-Lact-PER13-34]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSQQLT
VQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDAHAVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=434425 IMQ31_RS18265 WP_024230002.1 3859079..3860572(+) (pilB/pilB1) [Escherichia coli O167:H26 strain Res13-Lact-PER13-34]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCACGTTCATCCGCTATCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATCGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTAGACGGAACGCTACATGAAATGCTGCGT
CCCGGGCGCAAACTAGCCTCGCTGCTGGTGTCGCGTATCAAGGTGATGGCGCGGCTGGACATTGCCGAAAAGCGCGTGCC
GCAGGATGGACGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTGCGTGTCTCCACCATGCCTTCTGCCTGGGGCG
AGCGCGTGGTGCTGCGACTGCTGGACAAAAACCAGGCCCGCCTGACGCTGGAACGTCTGGGGTTGAGTCAGCAACTGACC
GTGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATCTTTCTGGTGACGGGGCCGACGGGTTCTGGCAAAAGCACCAC
GCTGTACGCCGGATTGCAGGAGCTGAACAACCATTCGCGTAACATTCTCACGGTTGAAGACCCCATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTCAACACCCGCGTCGGCATGACCTTCGCCCGTGGCCTGCGCGCGATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCCGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACAGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCATGCCGTCACTCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACTATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.372

100

0.429
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.059

85.513

0.402
  pilB Vibrio parahaemolyticus RIMD 2210633

48.363

79.879

0.386
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.617

80.282

0.374
  pilB Vibrio cholerae strain A1552

46.212

79.678

0.368
  pilF Thermus thermophilus HB27

45

80.483

0.362
  pilB Acinetobacter baylyi ADP1

45.897

78.471

0.36