Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   HB764_RS14055 Genome accession   NZ_CP050478
Coordinates   2986193..2986786 (-) Length   197 a.a.
NCBI ID   WP_255957169.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2981193..2991786
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB764_RS14035 (HB764_13925) aroB 2982066..2983166 (-) 1101 WP_255957168.1 3-dehydroquinate synthase -
  HB764_RS14040 (HB764_13930) aroK 2983198..2983716 (-) 519 WP_010443904.1 shikimate kinase AroK -
  HB764_RS14045 (HB764_13935) pilQ 2983905..2985653 (-) 1749 WP_255958200.1 type IV pilus secretin PilQ Machinery gene
  HB764_RS14050 (HB764_13940) pilP 2985688..2986203 (-) 516 WP_010643498.1 pilus assembly protein PilP Machinery gene
  HB764_RS14055 (HB764_13945) pilO 2986193..2986786 (-) 594 WP_255957169.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB764_RS14060 (HB764_13950) pilN 2986779..2987354 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  HB764_RS14065 (HB764_13955) pilM 2987338..2988354 (-) 1017 WP_255957170.1 type IV pilus biogenesis protein PilM Machinery gene
  HB764_RS14070 (HB764_13960) - 2988533..2991100 (+) 2568 WP_255957171.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 197 a.a.        Molecular weight: 22981.48 Da        Isoelectric Point: 4.9464

>NTDB_id=433222 HB764_RS14055 WP_255957169.1 2986193..2986786(-) (pilO) [Vibrio campbellii strain LJC011]
MVNWQDLEMDEIAEWPLVPQLVAILLMVVLIQGFGYWFYLKPEEARLNTLIHEEQTLKVALRIKANKVAALPQLQSQLDE
LTTRYELLLQQLPAQKELATMLASVNELGIDNKLTFTRIDWGDKESEHFLYRLPLNIELTGDFHDIGRFSQAIAALPRII
TFQDVTWQRVSQESETLHFRVRANTYQFKPEEKKDAK

Nucleotide


Download         Length: 594 bp        

>NTDB_id=433222 HB764_RS14055 WP_255957169.1 2986193..2986786(-) (pilO) [Vibrio campbellii strain LJC011]
ATGGTTAATTGGCAAGACCTAGAAATGGATGAAATCGCTGAATGGCCGCTGGTACCTCAGTTGGTGGCGATTCTTCTTAT
GGTGGTGTTGATCCAAGGTTTTGGTTACTGGTTTTATTTGAAGCCAGAGGAAGCTCGCCTTAATACCTTGATCCATGAAG
AGCAGACTTTAAAAGTCGCGCTGAGGATCAAAGCCAATAAAGTAGCTGCACTGCCGCAGCTTCAGTCACAACTTGATGAA
CTGACCACCCGCTATGAACTGCTTTTGCAACAACTGCCGGCACAGAAAGAATTGGCGACCATGCTAGCGTCGGTTAATGA
ACTCGGCATCGATAACAAGCTGACATTTACGCGTATTGATTGGGGAGATAAAGAGTCAGAGCACTTCCTTTACCGTTTGC
CGCTCAATATTGAGCTAACGGGTGACTTCCATGACATCGGTCGTTTTTCACAGGCCATCGCCGCCTTACCACGCATCATC
ACATTTCAAGATGTAACTTGGCAGCGAGTCAGTCAAGAGAGTGAAACCTTGCACTTCCGTGTAAGAGCAAACACTTACCA
ATTCAAACCAGAGGAAAAGAAAGATGCGAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(48-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio campbellii strain DS40M4

99.474

96.447

0.959
  pilO Vibrio cholerae strain A1552

71.728

96.954

0.695