Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   HB764_RS14065 Genome accession   NZ_CP050478
Coordinates   2987338..2988354 (-) Length   338 a.a.
NCBI ID   WP_255957170.1    Uniprot ID   -
Organism   Vibrio campbellii strain LJC011     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2982338..2993354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HB764_RS14040 (HB764_13930) aroK 2983198..2983716 (-) 519 WP_010443904.1 shikimate kinase AroK -
  HB764_RS14045 (HB764_13935) pilQ 2983905..2985653 (-) 1749 WP_255958200.1 type IV pilus secretin PilQ Machinery gene
  HB764_RS14050 (HB764_13940) pilP 2985688..2986203 (-) 516 WP_010643498.1 pilus assembly protein PilP Machinery gene
  HB764_RS14055 (HB764_13945) pilO 2986193..2986786 (-) 594 WP_255957169.1 type 4a pilus biogenesis protein PilO Machinery gene
  HB764_RS14060 (HB764_13950) pilN 2986779..2987354 (-) 576 WP_005432710.1 PilN domain-containing protein Machinery gene
  HB764_RS14065 (HB764_13955) pilM 2987338..2988354 (-) 1017 WP_255957170.1 type IV pilus biogenesis protein PilM Machinery gene
  HB764_RS14070 (HB764_13960) - 2988533..2991100 (+) 2568 WP_255957171.1 penicillin-binding protein 1A -
  HB764_RS14075 (HB764_13965) oxyR 2991179..2992087 (-) 909 WP_005432705.1 DNA-binding transcriptional regulator OxyR -
  HB764_RS14080 (HB764_13970) - 2992262..2992990 (+) 729 WP_255957173.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37812.24 Da        Isoelectric Point: 6.7006

>NTDB_id=433224 HB764_RS14065 WP_255957170.1 2987338..2988354(-) (pilM) [Vibrio campbellii strain LJC011]
MDKLLVTGIDIGHNSLKAVVLKPNGNSYALLGYKEIILKEGIVAENNTINHQEIVKTLKEMKKELPFRARKVAISVPDNA
VISKKLQIEQNLEESEKEFAVVQAFSHQSPFPVEELSLDFIKVDTDEQMRGNDSYQVFATRKDAVETRVEALQKSGLKPV
LVDVHSQSLGHIWKLVAKRFPEKNKYCLLDIGSLNSSFTMFTEQGELFHKEFPCGSRTTGSLFQQDPLADDAQVATEQFN
RQIVERVKRQMQLYTSINGSQDIKGVWLSGEGAATPMLAEELSSQLALECELLNPLGLFEMKVSKRKRRAADWQHFTAAA
GLAVRGIQWLGGDNAVSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=433224 HB764_RS14065 WP_255957170.1 2987338..2988354(-) (pilM) [Vibrio campbellii strain LJC011]
ATGGATAAGCTATTAGTTACGGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTGGTACTTAAACCCAATGGCAACAG
TTACGCACTACTGGGTTACAAAGAAATAATACTTAAGGAAGGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACCCTTAAAGAGATGAAAAAAGAGCTACCTTTTCGTGCTCGAAAGGTCGCAATTTCAGTTCCAGATAATGCT
GTGATCAGTAAAAAGCTGCAGATAGAGCAGAACTTAGAAGAGAGTGAAAAGGAGTTTGCTGTTGTGCAAGCCTTCTCACA
TCAGTCTCCATTCCCCGTGGAAGAGCTGAGTCTTGACTTCATCAAGGTTGATACGGATGAGCAGATGCGAGGCAATGACA
GTTATCAGGTCTTCGCGACTCGTAAAGACGCGGTAGAAACCCGAGTTGAGGCATTGCAGAAATCAGGCTTGAAACCAGTA
CTGGTGGACGTGCACTCACAAAGCCTCGGTCATATCTGGAAATTGGTTGCGAAACGTTTTCCTGAAAAGAACAAGTATTG
TTTACTGGACATTGGTTCTTTGAACAGTTCTTTCACGATGTTCACCGAACAAGGTGAATTGTTTCATAAAGAATTTCCCT
GCGGCTCTCGCACTACGGGCAGTTTGTTTCAACAAGATCCATTAGCAGACGATGCACAAGTGGCAACGGAGCAATTTAAT
CGACAAATCGTTGAGCGTGTTAAACGTCAGATGCAGCTCTATACCTCAATTAATGGAAGCCAGGATATCAAAGGCGTTTG
GCTTTCGGGAGAGGGCGCTGCAACACCTATGTTGGCCGAGGAGCTGTCTAGCCAGTTGGCACTAGAGTGTGAACTGCTTA
ATCCACTTGGTTTGTTTGAAATGAAGGTCTCTAAACGCAAACGTCGTGCCGCGGATTGGCAACACTTCACTGCTGCAGCC
GGACTGGCTGTACGTGGTATTCAGTGGCTAGGAGGTGACAATGCTGTATCACATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

99.112

100

0.991
  pilM Vibrio cholerae strain A1552

47.953

100

0.485