Detailed information NTG0432089

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: HBK86_RS16505
• Genome accession: NZ_CP050385
• Coordinates: 3383477..3384535 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain VB82
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3378477..3389535

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HBK86_RS16480 (HBK86_16480)	aroK	3378752..3379321 (-)	570	WP_000643712.1	shikimate kinase AroK	-
HBK86_RS16485 (HBK86_16485)	pilQ	3379333..3381498 (-)	2166	WP_001017029.1	type IV pilus secretin PilQ family protein	Machinery gene
HBK86_RS16490 (HBK86_16490)	pilP	3381561..3382088 (-)	528	WP_000695061.1	pilus assembly protein PilP	Machinery gene
HBK86_RS16495 (HBK86_16495)	pilO	3382099..3382839 (-)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
HBK86_RS16500 (HBK86_16500)	pilN	3382836..3383477 (-)	642	WP_000201221.1	PilN domain-containing protein	Machinery gene
HBK86_RS16505 (HBK86_16505)	pilM	3383477..3384535 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
HBK86_RS16510 (HBK86_16510)	ponA	3384697..3387252 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
HBK86_RS16515 (HBK86_16515)	-	3387270..3388094 (-)	825	WP_086245490.1	putative RNA methyltransferase	-
HBK86_RS16520 (HBK86_16520)	-	3388097..3388429 (-)	333	WP_000169599.1	hypothetical protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=432089 HBK86_RS16505 WP_002014066.1 3383477..3384535(-) (pilM) [Acinetobacter baumannii strain VB82]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=432089 HBK86_RS16505 WP_002014066.1 3383477..3384535(-) (pilM) [Acinetobacter baumannii strain VB82]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGACTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCAGAGCGAAAAAAGATCGTTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGCTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTATCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423