Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   HBL79_RS01200 Genome accession   NZ_CP050316
Coordinates   204875..205306 (+) Length   143 a.a.
NCBI ID   WP_035687024.1    Uniprot ID   A0A0F5ESJ8
Organism   Avibacterium paragallinarum strain ESV-135     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 199875..210306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HBL79_RS01170 (HBL79_01170) - 200031..200882 (-) 852 WP_017807045.1 thymidylate synthase -
  HBL79_RS01175 (HBL79_01175) lgt 200894..201694 (-) 801 WP_035687035.1 prolipoprotein diacylglyceryl transferase -
  HBL79_RS01180 (HBL79_01180) - 201704..202495 (-) 792 WP_035687031.1 sulfite exporter TauE/SafE family protein -
  HBL79_RS01185 (HBL79_01185) rppH 202498..203091 (-) 594 WP_035687029.1 RNA pyrophosphohydrolase -
  HBL79_RS01190 (HBL79_01190) - 203663..204082 (-) 420 WP_124994872.1 hypothetical protein -
  HBL79_RS01195 (HBL79_01195) ampD 204181..204735 (-) 555 WP_035687059.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  HBL79_RS01200 (HBL79_01200) pilA 204875..205306 (+) 432 WP_035687024.1 pilin Machinery gene
  HBL79_RS01205 (HBL79_01205) pilB 205319..206743 (+) 1425 WP_035687022.1 GspE/PulE family protein Machinery gene
  HBL79_RS01210 (HBL79_01210) pyrH 206993..207709 (+) 717 WP_035687020.1 UMP kinase -
  HBL79_RS01215 (HBL79_01215) frr 207734..208291 (+) 558 WP_017807054.1 ribosome recycling factor -
  HBL79_RS01220 (HBL79_01220) ispC 208471..209667 (+) 1197 WP_035687018.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Sequence

Protein


Download         Length: 143 a.a.        Molecular weight: 15205.70 Da        Isoelectric Point: 9.8115

>NTDB_id=431400 HBL79_RS01200 WP_035687024.1 204875..205306(+) (pilA) [Avibacterium paragallinarum strain ESV-135]
MQNPTHLKIKKGFTLIELMIVIAIVAILATIAIPAYQNYTKKAAISELIQAASPYRSEVELCIYNTGATTKCNAGSNGIQ
KKLTNKGKVASIDVQKGTITVKGKDNLSDITYSLKASGDASKGVSWTVNCGKSVEFFPAGFCG

Nucleotide


Download         Length: 432 bp        

>NTDB_id=431400 HBL79_RS01200 WP_035687024.1 204875..205306(+) (pilA) [Avibacterium paragallinarum strain ESV-135]
ATGCAAAACCCAACCCATTTAAAAATCAAAAAAGGCTTTACTTTGATTGAACTGATGATCGTGATTGCGATTGTGGCGAT
TTTAGCGACCATTGCGATTCCTGCCTATCAAAATTACACCAAGAAAGCGGCAATATCTGAGTTAATTCAAGCCGCAAGCC
CCTATCGTTCAGAGGTGGAATTATGTATTTATAATACGGGGGCTACCACGAAATGTAATGCGGGTTCAAATGGAATTCAG
AAAAAATTAACGAATAAAGGGAAAGTGGCTTCTATTGATGTACAAAAAGGCACGATTACGGTTAAAGGAAAAGATAATCT
TTCTGATATTACTTATTCATTAAAAGCCAGTGGAGATGCCTCAAAAGGTGTATCTTGGACGGTAAATTGTGGAAAATCTG
TAGAGTTTTTCCCAGCGGGATTTTGCGGATAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(42-128)

Prokaryotic N-terminal methylation motif (N_methyl)

(9-32)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5ESJ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Haemophilus influenzae Rd KW20

59.31

100

0.601
  pilA Haemophilus influenzae 86-028NP

57.931

100

0.587
  pilA Glaesserella parasuis strain SC1401

46.809

98.601

0.462
  pilA Vibrio campbellii strain DS40M4

34.615

100

0.378
  pilA Ralstonia pseudosolanacearum GMI1000

37.324

99.301

0.371