Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G8A07_RS06205 Genome accession   NZ_CP049919
Coordinates   1325609..1327336 (-) Length   575 a.a.
NCBI ID   WP_195796204.1    Uniprot ID   A0A7S9C531
Organism   Roseateles sp. DAIF2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1320609..1332336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G8A07_RS06175 (G8A07_06175) - 1321149..1321766 (+) 618 WP_195796200.1 HAD family hydrolase -
  G8A07_RS06180 (G8A07_06180) - 1321755..1321967 (-) 213 WP_195796201.1 DNA gyrase inhibitor YacG -
  G8A07_RS06185 (G8A07_06185) zapD 1321973..1322728 (-) 756 WP_195797621.1 cell division protein ZapD -
  G8A07_RS06190 (G8A07_06190) coaE 1322811..1323404 (-) 594 WP_249937244.1 dephospho-CoA kinase -
  G8A07_RS06195 (G8A07_06195) - 1323407..1324333 (-) 927 WP_195796202.1 A24 family peptidase -
  G8A07_RS06200 (G8A07_06200) pilC 1324352..1325572 (-) 1221 WP_195796203.1 type II secretion system F family protein Machinery gene
  G8A07_RS06205 (G8A07_06205) pilB 1325609..1327336 (-) 1728 WP_195796204.1 type IV-A pilus assembly ATPase PilB Machinery gene
  G8A07_RS06210 (G8A07_06210) - 1327570..1329957 (+) 2388 WP_195796205.1 tetratricopeptide repeat protein -
  G8A07_RS06220 (G8A07_06220) ispB 1330176..1331162 (-) 987 WP_371816435.1 octaprenyl diphosphate synthase -
  G8A07_RS06225 (G8A07_06225) rplU 1331360..1331671 (+) 312 WP_195797624.1 50S ribosomal protein L21 -
  G8A07_RS06230 (G8A07_06230) rpmA 1331712..1331969 (+) 258 WP_195796206.1 50S ribosomal protein L27 -

Sequence

Protein


Download         Length: 575 a.a.        Molecular weight: 62798.07 Da        Isoelectric Point: 5.5675

>NTDB_id=428683 G8A07_RS06205 WP_195796204.1 1325609..1327336(-) (pilB) [Roseateles sp. DAIF2]
MNVDTTLVEPPHSALSGVARVLVHAGKLQAKTAEDLVRQAREKKTTFVAAVMAANAVSATELAHTLSAALALPLLDLNAM
DAQRLPTNIIDSKLALQYQVIALGRRGNRLFIGGADPTDQEVVERIKFATQLAPEWVIVEYDKLVKQLQGSSASAAEALE
SIAGGDFDFDIAEEDTTPEQEAAEIADVEDAPVVRFLQKMLVDAINMRASDLHFEPYEYHYRVRFRVDGELREITQPPIA
IKDKLSSRIKVISRLDIAERRVPQDGRMKLKFGAKAIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEKIEKDRLL
ACIHRPYGMILVTGPTGSGKTVSLYTCLNILNQPGVNISTVEDPAEINLPGINQVNVNDKAGLTFSAALKSFLRQDPDII
MVGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRLLNMGVAPFNIASSVLLITAQRLARRLCEHCKAPADYPHDA
LLRAGYAEEELDGSWKPYRAVGCSSCNNGYRGRVGLYQVMPVTEAIQRIILSEGTAMDIAEQARSEGVRDLRQSGLVKVR
AGVTTLEEVLAATNE

Nucleotide


Download         Length: 1728 bp        

>NTDB_id=428683 G8A07_RS06205 WP_195796204.1 1325609..1327336(-) (pilB) [Roseateles sp. DAIF2]
ATGAACGTGGATACCACCCTGGTCGAGCCGCCCCATTCCGCCCTGTCCGGCGTCGCCCGCGTGCTGGTGCATGCCGGCAA
GCTGCAGGCCAAGACCGCCGAGGATCTGGTGCGTCAGGCGCGCGAGAAGAAGACCACCTTCGTCGCCGCGGTGATGGCGG
CCAATGCGGTCTCGGCCACCGAGCTGGCGCATACCCTGTCCGCAGCGTTGGCGTTGCCGCTGCTGGACCTCAACGCGATG
GACGCGCAGCGCCTGCCGACCAATATCATCGACAGCAAGCTCGCGCTGCAGTATCAGGTGATCGCGCTGGGCCGGCGCGG
CAACCGCCTCTTCATCGGCGGCGCAGACCCGACCGACCAGGAGGTGGTGGAGCGCATCAAGTTCGCCACCCAGCTGGCGC
CCGAATGGGTCATCGTCGAGTACGACAAGCTGGTCAAGCAGTTGCAGGGCAGCAGCGCCAGCGCGGCCGAGGCGCTGGAA
TCGATCGCCGGCGGCGACTTCGATTTCGACATCGCCGAGGAAGACACCACTCCTGAGCAGGAGGCGGCCGAGATCGCCGA
TGTGGAAGACGCGCCGGTGGTGCGCTTCCTGCAGAAGATGCTGGTGGATGCGATCAATATGCGCGCCTCGGACCTGCACT
TCGAGCCCTACGAGTACCATTACCGGGTGCGCTTCCGCGTCGATGGCGAGCTGCGCGAGATCACGCAGCCACCCATTGCG
ATCAAGGACAAGCTGTCCTCGCGCATCAAGGTGATCTCGCGCCTGGACATCGCCGAGCGCCGCGTGCCACAGGACGGCCG
CATGAAGCTGAAGTTCGGCGCCAAGGCGATCGACTTCCGCGTCAGCACCCTGCCGACCCTGTTCGGCGAGAAGATCGTGA
TCCGGATCCTGGACCCCTCCTCGGCCAAGCTGGGCATCGAGGCGCTGGGCTACGAGAAGATCGAGAAAGACCGCCTGCTC
GCCTGCATCCACCGCCCCTACGGCATGATCCTGGTGACCGGTCCGACCGGCTCGGGCAAGACGGTGTCGCTCTACACCTG
CCTGAACATCCTGAACCAGCCGGGCGTCAACATCTCCACCGTCGAGGACCCGGCAGAAATCAACCTGCCGGGCATCAACC
AGGTCAATGTCAACGACAAGGCCGGCCTGACCTTCTCGGCCGCGCTGAAGTCCTTCCTGCGCCAGGATCCGGACATCATC
ATGGTCGGCGAGATCCGCGACCTGGAGACGGCCGACATCGCGATCAAGGCCGCCCAGACCGGCCATATGGTGATGTCGAC
CCTGCACACCAACGACGCGCCGACCACGCTGACCCGCCTGCTGAACATGGGCGTCGCACCCTTCAACATTGCGTCCAGCG
TGCTGCTGATCACCGCCCAGCGGCTGGCACGGCGCCTGTGCGAACACTGCAAGGCGCCGGCCGACTATCCACATGACGCT
TTGCTGCGCGCCGGCTATGCCGAGGAGGAGCTGGACGGCAGCTGGAAGCCCTACCGTGCCGTCGGCTGCTCAAGTTGCAA
CAACGGGTATCGAGGACGTGTCGGTCTTTACCAAGTGATGCCGGTCACGGAGGCGATTCAACGCATCATCCTGTCCGAGG
GAACGGCCATGGACATCGCCGAGCAGGCGCGCAGCGAAGGCGTGCGCGATCTGCGGCAATCCGGGCTGGTCAAGGTCAGG
GCCGGCGTCACGACACTGGAAGAAGTGCTGGCGGCCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-149)

Type II/IV secretion system protein (T2SSE)

(199-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7S9C531

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.641

97.913

0.525
  pilF Neisseria gonorrhoeae MS11

52.743

98.261

0.518
  pilB Acinetobacter baylyi ADP1

51.134

99.652

0.51
  pilB Legionella pneumophila strain ERS1305867

50.613

99.304

0.503
  pilB Vibrio cholerae strain A1552

46.085

97.739

0.45
  pilB Vibrio parahaemolyticus RIMD 2210633

45.167

98.957

0.447
  pilB Vibrio campbellii strain DS40M4

45.552

97.739

0.445