Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   G7083_RS07580 Genome accession   NZ_CP049913
Coordinates   1559271..1559861 (+) Length   196 a.a.
NCBI ID   WP_166014234.1    Uniprot ID   A0A6G8D410
Organism   Vibrio sp. HDW18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1554271..1564861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7083_RS07565 (G7083_07565) - 1555059..1557536 (-) 2478 WP_166014231.1 penicillin-binding protein 1A -
  G7083_RS07570 (G7083_07570) pilM 1557688..1558704 (+) 1017 WP_166014232.1 type IV pilus assembly protein PilM Machinery gene
  G7083_RS07575 (G7083_07575) pilN 1558694..1559278 (+) 585 WP_166014233.1 PilN domain-containing protein Machinery gene
  G7083_RS07580 (G7083_07580) pilO 1559271..1559861 (+) 591 WP_166014234.1 type 4a pilus biogenesis protein PilO Machinery gene
  G7083_RS07585 (G7083_07585) pilP 1559851..1560369 (+) 519 WP_166014235.1 pilus assembly protein PilP Machinery gene
  G7083_RS07590 (G7083_07590) pilQ 1560395..1562107 (+) 1713 WP_166014236.1 type IV pilus secretin PilQ family protein Machinery gene
  G7083_RS07595 (G7083_07595) aroK 1562294..1562818 (+) 525 WP_166014237.1 shikimate kinase AroK -
  G7083_RS07600 (G7083_07600) aroB 1562849..1563934 (+) 1086 WP_166014238.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 23033.38 Da        Isoelectric Point: 4.6424

>NTDB_id=428606 G7083_RS07580 WP_166014234.1 1559271..1559861(+) (pilO) [Vibrio sp. HDW18]
MTNWQELELDEITEWPLLPQLLVILLLMLLVQGAGYWFYLMPQQDQINALKQEEQTVKATLRIKANKVAALPQIQAQLDE
LKQRYDFLLRQLPVQKELASMLASVNQLGLDNSLTFTRIDWGERQNQAFLYRLPLNIELTGNYHDIGDFSQAIAQLPRII
HFDDIDWQRVSQESSTLHFRVRAYTYQFKPEVEDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=428606 G7083_RS07580 WP_166014234.1 1559271..1559861(+) (pilO) [Vibrio sp. HDW18]
ATGACTAATTGGCAAGAATTGGAACTTGATGAGATCACCGAATGGCCATTATTGCCTCAGTTATTGGTGATTTTATTGTT
GATGTTGCTAGTTCAAGGGGCGGGTTACTGGTTTTATCTGATGCCACAACAAGATCAAATCAACGCTTTGAAACAAGAAG
AGCAAACGGTTAAAGCCACTTTGAGGATCAAAGCCAACAAAGTGGCCGCTTTGCCACAGATTCAGGCGCAACTCGATGAA
TTAAAACAGCGCTATGACTTTTTATTACGTCAATTGCCGGTACAAAAAGAGCTGGCCAGTATGCTGGCGTCAGTCAATCA
ATTAGGCCTAGATAACTCACTGACCTTCACCAGAATTGATTGGGGTGAACGCCAAAATCAAGCCTTTCTGTATCGTTTAC
CACTCAATATTGAGCTGACCGGTAACTATCACGATATTGGTGATTTCTCTCAGGCGATTGCCCAACTGCCACGGATTATT
CATTTTGATGACATTGATTGGCAGCGAGTCAGTCAAGAAAGCAGCACACTGCATTTTCGAGTCAGAGCCTACACCTATCA
GTTTAAGCCGGAGGTAGAGGATGAAAAATAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8D410

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

87.755

100

0.878
  pilO Vibrio campbellii strain DS40M4

72.632

96.939

0.704