Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   G7083_RS07570 Genome accession   NZ_CP049913
Coordinates   1557688..1558704 (+) Length   338 a.a.
NCBI ID   WP_166014232.1    Uniprot ID   A0A6G8D3Q3
Organism   Vibrio sp. HDW18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1552688..1563704
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7083_RS07555 (G7083_07555) - 1553230..1553961 (-) 732 WP_166014229.1 glutathione peroxidase -
  G7083_RS07560 (G7083_07560) oxyR 1554101..1555006 (+) 906 WP_166014230.1 DNA-binding transcriptional regulator OxyR -
  G7083_RS07565 (G7083_07565) - 1555059..1557536 (-) 2478 WP_166014231.1 penicillin-binding protein 1A -
  G7083_RS07570 (G7083_07570) pilM 1557688..1558704 (+) 1017 WP_166014232.1 type IV pilus assembly protein PilM Machinery gene
  G7083_RS07575 (G7083_07575) pilN 1558694..1559278 (+) 585 WP_166014233.1 PilN domain-containing protein Machinery gene
  G7083_RS07580 (G7083_07580) pilO 1559271..1559861 (+) 591 WP_166014234.1 type 4a pilus biogenesis protein PilO Machinery gene
  G7083_RS07585 (G7083_07585) pilP 1559851..1560369 (+) 519 WP_166014235.1 pilus assembly protein PilP Machinery gene
  G7083_RS07590 (G7083_07590) pilQ 1560395..1562107 (+) 1713 WP_166014236.1 type IV pilus secretin PilQ family protein Machinery gene
  G7083_RS07595 (G7083_07595) aroK 1562294..1562818 (+) 525 WP_166014237.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37574.04 Da        Isoelectric Point: 5.9196

>NTDB_id=428604 G7083_RS07570 WP_166014232.1 1557688..1558704(+) (pilM) [Vibrio sp. HDW18]
MGKSLVTGIDIGHHSIKAVVLKPMGDTYALVGYEELLVTADIFTDNYTLDYQKIVKKLKQLKKGLPLFSRKVALAIPDNA
VISKSLPIDSDLEPREQEFAIYQAFAHQSPFPVEDLSFDFVEIASSAVARSSTTTFQVYATRTEVVDSRWQVSKKAGFEP
ILIDVQVHSLLKLWQHASRAQRRADWMLIDIGYSQSTLCLDWPDKMPFYKDIPLGTQRLDVEPRSEFAVNPSELQDPTQR
FIDELVDKTARQIQLFTSVHGAQNLSGLWLSGGGALMAGLTEALQQRVGLPCEVLDPLSLFTIEVARRQRPVRDGARFST
AAGLALCGLAWLEGEHAG

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=428604 G7083_RS07570 WP_166014232.1 1557688..1558704(+) (pilM) [Vibrio sp. HDW18]
ATGGGTAAATCATTAGTTACGGGTATTGATATCGGCCATCACAGCATTAAAGCTGTAGTGCTCAAACCTATGGGCGACAC
CTACGCGCTGGTGGGGTATGAGGAGTTGCTCGTTACCGCGGATATTTTCACCGATAACTACACGCTCGATTATCAGAAAA
TTGTAAAGAAACTCAAACAACTAAAAAAGGGGTTACCGCTATTTAGTCGTAAAGTCGCGCTGGCAATTCCGGATAATGCG
GTGATCAGCAAATCATTACCCATTGATAGTGACCTTGAACCGCGTGAGCAGGAGTTTGCTATCTATCAGGCTTTCGCCCA
TCAATCCCCTTTTCCGGTCGAAGATCTGAGTTTCGATTTTGTTGAGATCGCGAGTAGTGCTGTTGCGCGCAGCAGCACGA
CAACGTTCCAAGTTTATGCCACCAGAACTGAAGTGGTCGACAGTCGTTGGCAAGTCAGTAAGAAAGCGGGTTTTGAGCCG
ATCTTGATCGATGTACAAGTGCATAGCTTGCTGAAGTTATGGCAACACGCGAGTCGTGCTCAGCGGCGTGCGGATTGGAT
GTTGATTGATATTGGTTATAGCCAAAGCACCCTGTGTCTCGATTGGCCAGACAAAATGCCGTTTTACAAAGATATTCCGC
TCGGTACTCAACGACTCGACGTCGAGCCAAGGAGTGAATTTGCCGTCAACCCATCAGAGTTGCAAGATCCAACACAGCGC
TTTATTGATGAGTTGGTCGATAAAACGGCCCGTCAAATTCAGTTATTTACCTCGGTACATGGGGCGCAGAATCTGAGTGG
CCTATGGCTTTCTGGTGGTGGCGCACTGATGGCGGGGTTGACTGAGGCCCTGCAGCAGCGTGTAGGGCTACCCTGTGAGG
TATTGGATCCATTATCACTGTTCACCATCGAGGTAGCGAGGCGTCAGCGACCAGTGAGGGATGGGGCACGGTTTAGCACC
GCGGCAGGTTTGGCCTTGTGTGGTTTAGCGTGGCTGGAGGGTGAGCATGCTGGATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G8D3Q3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

76.036

100

0.76
  pilM Vibrio campbellii strain DS40M4

44.118

100

0.444