Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G7079_RS12185 Genome accession   NZ_CP049872
Coordinates   2566590..2568314 (+) Length   574 a.a.
NCBI ID   WP_166057564.1    Uniprot ID   A0A6G7ZXG8
Organism   Thermomonas sp. HDW16     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2548809..2566460 2566590..2568314 flank 130


Gene organization within MGE regions


Location: 2548809..2568314
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7079_RS13480 (G7079_12100) - 2548809..2549138 (+) 330 WP_166057545.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  G7079_RS12100 (G7079_12105) - 2549273..2551144 (+) 1872 WP_166057546.1 hypothetical protein -
  G7079_RS12105 (G7079_12110) gmd 2551205..2552314 (+) 1110 WP_166057547.1 GDP-mannose 4,6-dehydratase -
  G7079_RS12110 (G7079_12115) - 2552320..2553258 (+) 939 WP_166057548.1 GDP-L-fucose synthase -
  G7079_RS12115 (G7079_12120) - 2553285..2554724 (+) 1440 WP_166057549.1 lipopolysaccharide biosynthesis protein -
  G7079_RS12120 (G7079_12125) - 2554714..2555616 (+) 903 WP_166057550.1 class I SAM-dependent methyltransferase -
  G7079_RS12125 (G7079_12130) - 2555702..2556214 (+) 513 WP_166057551.1 class I SAM-dependent methyltransferase -
  G7079_RS12130 (G7079_12135) - 2556211..2557200 (+) 990 WP_166057552.1 glycosyltransferase family 2 protein -
  G7079_RS12135 (G7079_12140) - 2557200..2558120 (+) 921 WP_166057553.1 class I SAM-dependent methyltransferase -
  G7079_RS12140 (G7079_12145) - 2558117..2558557 (+) 441 WP_166057555.1 hypothetical protein -
  G7079_RS12145 (G7079_12150) - 2558554..2559675 (+) 1122 WP_166057556.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  G7079_RS12150 (G7079_12155) - 2559672..2560430 (+) 759 WP_166057557.1 class I SAM-dependent methyltransferase -
  G7079_RS12155 (G7079_12160) - 2560427..2561632 (+) 1206 WP_166057558.1 glycosyltransferase family 4 protein -
  G7079_RS12160 (G7079_12165) - 2561649..2562413 (+) 765 WP_166057559.1 glycosyltransferase family 2 protein -
  G7079_RS12165 (G7079_12170) - 2562567..2563604 (-) 1038 WP_166057560.1 glycosyltransferase family 4 protein -
  G7079_RS12170 (G7079_12175) - 2563743..2564456 (-) 714 WP_166057561.1 class I SAM-dependent methyltransferase -
  G7079_RS12175 (G7079_12180) - 2564456..2565538 (-) 1083 WP_166057562.1 glycosyltransferase family 4 protein -
  G7079_RS12180 (G7079_12185) - 2565591..2566460 (-) 870 WP_166057563.1 glycosyltransferase family 2 protein -
  G7079_RS12185 (G7079_12190) pilB 2566590..2568314 (+) 1725 WP_166057564.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 62553.98 Da        Isoelectric Point: 6.3081

>NTDB_id=428310 G7079_RS12185 WP_166057564.1 2566590..2568314(+) (pilB) [Thermomonas sp. HDW16]
MSATASANLMGITGIARRLVLDGAMDEAAARKAMDAATKERIPLATYIADKKLANPAALAAAYSMEFGIPLVDSLALDPN
QSAIRLVKEDLVRKHQALPLFKRGGRLFVGVADPTNHAALEEIKFHTNLAVEPILIDAERLKRSIDGWLEAAEDLADGMG
DADGLEGLEVSGGDEDFSGDSGVDAGGDDTPVVKFINKMLVDAIRKGASDIHFEPYETEYRVRFRIDGILKTIKRVPVKL
HQRISARLKVMAQLDIAEKRVPQDGRIKLNLSKSKQIDFRVSTLPTLFGEKVVLRILDGSAARLGIEKLGYEPDQQKLFV
DAVKRPYGMVLVTGPTGSGKTVSLYTALNILNEDERNISTVEDPVEIRVPGINQVQMNVKRGMTFAAALRSFLRQDPDVL
MVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGVAPYNITSSVSLVIAQRLLRRLHDCKRVVHLPEHAL
LAEGFTADEVHAGINVYEAVGCEDCTEGYKGRAGVYQVMPMTDEIQQIVLAGGNVQQITEAALASGVRDLRRSALDKVKQ
GVTSLIEINRVTKD

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=428310 G7079_RS12185 WP_166057564.1 2566590..2568314(+) (pilB) [Thermomonas sp. HDW16]
ATGAGCGCAACTGCATCCGCCAATCTGATGGGAATCACCGGCATCGCCCGGCGCCTGGTGCTGGACGGTGCCATGGACGA
GGCCGCCGCGCGCAAGGCGATGGACGCGGCGACCAAGGAACGCATCCCGTTGGCGACCTACATCGCCGACAAGAAGCTGG
CGAATCCTGCGGCACTGGCAGCGGCCTATTCAATGGAATTCGGCATTCCGTTGGTCGATTCGCTGGCCCTCGACCCCAAC
CAATCCGCAATTCGGCTGGTAAAGGAAGACCTAGTCCGCAAGCATCAGGCACTTCCGTTGTTCAAGCGCGGCGGTCGCCT
TTTCGTGGGCGTGGCGGATCCGACCAATCATGCGGCGCTGGAGGAAATCAAGTTCCACACCAATTTGGCTGTTGAGCCGA
TCCTGATTGATGCCGAACGGCTCAAGCGCAGCATCGATGGATGGCTGGAGGCTGCGGAGGACCTGGCCGATGGGATGGGT
GACGCCGATGGCCTCGAAGGCCTGGAGGTCAGCGGCGGAGACGAAGACTTCTCCGGCGATTCGGGCGTGGATGCGGGTGG
AGACGACACACCCGTCGTCAAGTTCATCAACAAGATGCTGGTGGATGCCATTCGCAAGGGCGCATCGGACATCCACTTCG
AACCGTATGAAACAGAGTATCGGGTTCGCTTCCGCATCGACGGCATCCTGAAGACGATCAAGCGGGTACCGGTCAAGCTG
CATCAACGCATCTCTGCTCGCCTGAAGGTGATGGCGCAGTTGGACATCGCCGAGAAACGGGTGCCGCAGGACGGTCGCAT
CAAACTCAACCTGTCCAAGAGCAAGCAGATCGATTTCCGCGTCAGCACTTTACCCACCTTGTTCGGTGAAAAAGTGGTGC
TGCGCATTCTGGATGGCAGCGCGGCCAGGCTCGGCATCGAGAAACTGGGCTACGAGCCGGACCAGCAGAAGCTGTTCGTG
GACGCGGTCAAGCGCCCCTATGGCATGGTCCTGGTCACCGGCCCCACCGGCTCCGGCAAGACCGTGAGCCTGTACACCGC
GCTCAACATCCTCAACGAGGACGAGCGCAACATCAGCACGGTCGAGGATCCCGTTGAAATCCGCGTTCCCGGCATCAACC
AGGTACAAATGAACGTGAAGCGCGGCATGACCTTTGCCGCCGCCTTGCGCAGTTTCCTGCGCCAGGATCCGGACGTGCTG
ATGGTGGGCGAAATCCGCGACCTGGAAACTGCCGAGATCGCGATCAAGGCGGCACAGACCGGCCACATGGTGTTGTCCAC
CCTGCATACCAACGACGCCCCGCAAACCATCGCTCGCCTGATGAACATGGGTGTGGCCCCGTACAACATCACCAGCTCGG
TCAGCCTGGTCATCGCCCAGCGCCTTTTGCGCCGCCTGCACGATTGCAAGCGCGTGGTGCATCTCCCGGAACATGCGCTG
CTGGCGGAGGGCTTCACCGCCGACGAAGTCCATGCCGGCATCAACGTGTACGAGGCAGTCGGCTGCGAAGACTGCACGGA
AGGTTACAAGGGGCGGGCCGGCGTCTACCAGGTCATGCCGATGACCGACGAGATCCAGCAGATCGTGTTGGCCGGTGGTA
ATGTCCAACAAATCACCGAGGCCGCCCTCGCCAGTGGCGTCCGCGACCTGCGCCGTTCCGCGTTGGACAAGGTCAAGCAG
GGCGTCACCAGCCTGATCGAAATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(51-148)

Type II/IV secretion system protein (T2SSE)

(198-465)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6G7ZXG8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

53.697

98.955

0.531
  pilB Legionella pneumophila strain ERS1305867

52.641

98.955

0.521
  pilB Acinetobacter baylyi ADP1

52.92

98.432

0.521
  pilB Vibrio cholerae strain A1552

48.084

100

0.481
  pilF Neisseria gonorrhoeae MS11

48.987

94.599

0.463
  pilB Vibrio campbellii strain DS40M4

52.772

84.843

0.448
  pilB Vibrio parahaemolyticus RIMD 2210633

52.342

85.54

0.448
  pilB/pilB1 Synechocystis sp. PCC 6803

39.139

93.031

0.364