Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   G7069_RS01260 Genome accession   NZ_CP049864
Coordinates   258649..260391 (+) Length   580 a.a.
NCBI ID   WP_205758729.1    Uniprot ID   -
Organism   Lysobacter sp. HDW10     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 247109..258580 258649..260391 flank 69


Gene organization within MGE regions


Location: 247109..260391
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  G7069_RS01210 (G7069_01210) - 247109..248950 (+) 1842 WP_166293455.1 hypothetical protein -
  G7069_RS01215 (G7069_01215) - 248947..250077 (+) 1131 WP_166293457.1 glycosyltransferase family 4 protein -
  G7069_RS01220 (G7069_01220) - 250074..250997 (+) 924 WP_166293459.1 NAD-dependent epimerase/dehydratase family protein -
  G7069_RS01225 (G7069_01225) - 250970..252004 (+) 1035 WP_166293462.1 glycosyltransferase family 2 protein -
  G7069_RS01230 (G7069_01230) - 252126..253076 (-) 951 WP_166293464.1 glycosyltransferase -
  G7069_RS01235 (G7069_01235) - 253073..254554 (-) 1482 WP_240912600.1 lipopolysaccharide biosynthesis protein -
  G7069_RS01240 (G7069_01240) - 254597..255391 (-) 795 WP_166293468.1 class I SAM-dependent methyltransferase -
  G7069_RS01245 (G7069_01245) - 255388..256296 (-) 909 WP_166293470.1 hypothetical protein -
  G7069_RS01250 (G7069_01250) - 256552..257718 (-) 1167 WP_166293472.1 DegT/DnrJ/EryC1/StrS family aminotransferase -
  G7069_RS01255 (G7069_01255) - 257723..258580 (-) 858 WP_166293474.1 glycosyltransferase family 2 protein -
  G7069_RS01260 (G7069_01260) pilB 258649..260391 (+) 1743 WP_205758729.1 type IV-A pilus assembly ATPase PilB Machinery gene

Sequence

Protein


Download         Length: 580 a.a.        Molecular weight: 62817.59 Da        Isoelectric Point: 5.0545

>NTDB_id=428236 G7069_RS01260 WP_205758729.1 258649..260391(+) (pilB) [Lysobacter sp. HDW10]
MAVNSVAATNLTGITGITRRLAIDGLIDPDVAREAQTAASAQRKPIARYLVENNLATSPQVLGAISAEFGIPLFDPSAMD
PNQSALKLVSEDLINKHNIIPLFKRGGKLFVGTADPANFAATEDLRFTTNLSVEMILVDEDQIKRTVEAWQTASGGNFDA
MMGGDGGDYDNLDVSAGDDEDNGGDKGIEAGGSDDAPVVKFVNKVLIDAIRRGASDIHFEPFENDYRVRLRVDGLLKNVA
TIPNKMHSRIAARLKVMAQLDIAEKRIPQDGRIKLNISKTKQMDFRVSTLPTLFGEKVVLRLLDGSAAKLGIDKLGYEDD
QRKLFEDAIQKPYGMVLVTGPTGSGKTVSLYTALNILNDETRNISTVEDPVEIRVAGINQVQQNNKRGMTFAAALRSFLR
QDPDVIMVGEIRDLETAEIAVKAAQTGHMVLSTLHTNDAPQTISRLMNMGIAPFNITSSVTLVIAQRLARRLHDCKQPVT
LPEAALIAEGFTEAEVAEGITLYEAKGCPDCTDGYKGRTGIYQVMPMTEEIQAIVLQGGNAMQIAEAAERSGVNDLRKSA
LLKAKNGVTSLNEINRVTKD

Nucleotide


Download         Length: 1743 bp        

>NTDB_id=428236 G7069_RS01260 WP_205758729.1 258649..260391(+) (pilB) [Lysobacter sp. HDW10]
ATGGCAGTGAATAGTGTCGCCGCAACCAACCTGACGGGCATTACTGGCATCACGCGTCGCTTGGCGATCGATGGTCTCAT
TGACCCCGACGTCGCGCGCGAGGCGCAGACGGCCGCCAGCGCACAGCGCAAGCCGATTGCGCGCTATTTGGTTGAGAACA
ATCTAGCGACCTCTCCCCAGGTATTGGGCGCCATCTCAGCCGAGTTTGGGATCCCGCTCTTCGATCCGAGTGCAATGGAT
CCAAACCAGTCAGCCCTAAAGCTGGTTTCAGAAGATCTGATTAATAAGCACAACATCATTCCGTTGTTTAAGAGGGGCGG
CAAGCTCTTTGTAGGCACGGCCGATCCGGCAAACTTCGCCGCGACAGAAGACCTCCGGTTCACTACCAATCTTAGTGTTG
AGATGATTCTGGTCGACGAAGACCAGATCAAGCGCACCGTCGAGGCCTGGCAAACCGCATCGGGCGGCAATTTCGACGCC
ATGATGGGTGGCGATGGCGGAGACTACGACAACCTTGATGTCTCCGCTGGTGATGACGAAGATAACGGTGGCGACAAAGG
CATCGAAGCCGGTGGCAGCGACGATGCGCCCGTCGTCAAATTCGTCAATAAGGTATTGATCGACGCCATTCGCCGCGGTG
CATCAGACATCCACTTTGAACCCTTCGAGAACGACTACCGAGTGCGCCTGCGTGTCGACGGCCTGCTGAAGAATGTCGCA
ACGATCCCCAACAAGATGCATTCTCGGATCGCGGCGCGTCTGAAGGTGATGGCCCAACTCGATATCGCCGAAAAGCGTAT
TCCGCAAGACGGCCGGATCAAGCTCAATATCTCGAAGACCAAGCAGATGGACTTCCGGGTCAGTACCTTGCCCACCCTGT
TTGGTGAAAAGGTCGTGCTTCGCCTTTTGGATGGCAGCGCCGCCAAACTGGGCATTGACAAGCTGGGCTACGAAGACGAC
CAACGCAAACTCTTCGAAGACGCAATCCAAAAACCATACGGAATGGTACTGGTTACGGGCCCCACCGGCTCGGGTAAGAC
AGTCTCGCTCTACACCGCACTGAATATCTTGAACGACGAAACCCGAAACATCTCAACCGTGGAAGACCCGGTTGAAATTC
GTGTGGCGGGCATCAACCAAGTCCAACAGAACAACAAACGCGGCATGACCTTCGCCGCAGCGCTCCGCAGCTTCTTGCGC
CAAGATCCTGACGTCATCATGGTCGGTGAAATCCGTGACTTGGAAACCGCGGAAATCGCCGTCAAAGCCGCTCAAACCGG
TCACATGGTGCTCTCGACGCTTCACACCAACGACGCCCCGCAAACCATCTCGCGTTTGATGAACATGGGCATCGCGCCCT
TTAACATCACCTCGTCCGTGACCTTGGTGATCGCTCAGCGCCTCGCGCGGCGCTTGCATGACTGCAAGCAGCCTGTCACT
CTGCCGGAAGCGGCACTGATTGCCGAGGGGTTTACAGAGGCAGAGGTTGCGGAAGGCATCACTTTGTATGAAGCCAAGGG
TTGCCCGGATTGCACCGATGGTTACAAGGGGCGTACAGGTATTTATCAGGTCATGCCTATGACCGAAGAGATTCAAGCCA
TCGTGTTGCAGGGGGGCAACGCGATGCAAATTGCCGAGGCAGCGGAACGGAGTGGCGTAAACGATCTACGCAAATCCGCG
CTGTTGAAAGCGAAAAACGGGGTGACAAGCCTCAATGAAATCAACCGCGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-149)

Type II/IV secretion system protein (T2SSE)

(204-471)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.371

98.621

0.536
  pilB Acinetobacter baylyi ADP1

53.287

99.655

0.531
  pilB Legionella pneumophila strain ERS1305867

51.13

99.138

0.507
  pilB Vibrio cholerae strain A1552

48.084

98.966

0.476
  pilB Vibrio campbellii strain DS40M4

46.14

98.276

0.453
  pilF Neisseria gonorrhoeae MS11

48.864

91.034

0.445
  pilB Vibrio parahaemolyticus RIMD 2210633

47.243

93.793

0.443
  pilF Thermus thermophilus HB27

38.256

96.897

0.371